Biophysical Society 2020 Annual Meeting

812-plat 9:45 am AQUAPORIN 0A IS REQUIRED FOR WATER HOMEOSTASIS IN THE ZEBRAFISH LENS IN VIVO .  Irene Vorontsova , Alexander Vallmitjana, Yosuke Nakazawa, Belén Torrado, Thomas Schilling, James E. Hall, Enrico Gratton, Leonel S. Malacrida 813-plat 10:00 am Travel Awardee RAPID AND EXTREME LOW-LIGHT SUPERRESOLUTION IMAGING VIA ARTIFICIAL INTELLIGENCE.  Bei Liu , Luhong Jin, Bowei Dong, Ruiyan Song, Fenqiang Zhao, Stephen Hahn, Timothy C. Elston, Yingke Xu, Klaus M. Hahn Platform Co-Chairs Rikard Blunck, Université de Montréal, Canada Kanchan Gupta, NIH, NINDS 814-plat 8:15 am SIMULATING STREAMING POTENTIALS IN POTASSIUM CHANNELS.  Csaba Daday , Wojciech Kopec, Bert L. de Groot 815-plat 8:30 am ASYMMETRIC MUTATIONS IN SELECTIVITY FILTER OF K + CHANNEL PORE GENERATE C-TYPE INACTIVATION. Marietheres Kleuter, Gerhard Thiel, Oliver Rauh 816-plat 8:45 am REFINEMENT OF HIGH-RESOLUTION CRYO-EM STRUCTURE OF HERG: WHAT CAN WE EXPECT?  Hanif Muhammad Khan , Peter D. Tieleman, Sergei Y. Noskov 817-plat 9:00 am IDENTIFICATION OF PUFA INTERACTION SITES ON A CARDIAC POTAS- SIUM CHANNEL.  Samira Yazdi, Johan E. Larsson , Williams E. Miranda, Valentina Corradi, Peter D. Tieleman, Sergei Y. Noskov, Peter H. Larsson, Sara I. Liin 818-plat 9:15 am DYNAMICS OF THE PAS AND CNBHD DOMAIN INTERACTION PROBED WITH A FLUORESCENT NONCANONICAL AMINO ACID (L-ANAP) IN HERG POTASSIUM CHANNELS.  Ashley A. Johnson , Matt C. Trudeau 819-plat 9:30 am STATE-DEPENDENT PHOTOCROSSLINKING AT THE BK CHANNEL INTER- SUBUNIT INTERFACE.  Alberto Jesus Gonzalez Hernandez , Belinda Rive- ro-Perez, David Bartolome-Martin, Diego Alvarez de la Rosa, Andrew J.R. Plested, Teresa Giráldez 820-plat 9:45 am POSITION OF INACTIVATION PARTICLE OF SHAKER KV CHANNELS IN RESTING STATE.  Roshan Pandey , Tanja U. Kalstrup, Rikard Blunck 821-plat 10:00 am EXPLORING STRUCTURAL DYNAMICS OF A MEMBRANE PROTEIN BY COMBINING BIOORTHOGONAL CHEMISTRY AND CYSTEINE MUTAGEN- ESIS.  Kanchan Gupta , Gilman E.S. Toombes, Kenton J. Swartz Platform Protein Dynamics and Allostery I 8:15 am - 10:15 am, Room 31ABC Co-Chairs Rodrigo Maillard, Georgetown University Anubhuti Singh, Technical University of Munich, Germany Voltage-gated K Channels 8:15 am - 10:15 am, Room 30ABC

822-plat 8:15 am UNDERSTANDING ALLOSTERIC INFORMATION TRANSFER ACROSS TIME- AND LENGTH SCALES.  Steffen Wolf , Benedikt Sohmen, Bjorn Hellen- kamp, Johann Thurn, Thorsten Hugel, Gerhard Stock 823-plat 8:30 am SINGLE MOLECULE DYNAMICS OF AN HSP70 CHAPERONE.  Anubhuti Singh , Soumit S. Mandal, Gabriel Žoldák, Matthias Rief 824-plat 8:45 am VISUALIZING DOMAIN MOTIONS IN NF-ΚB TRANSCRIPTIONAL REGULA- TION. Wei Chen , Elizabeth A. Komives 825-plat 9:00 am CATCHING FAST PROTEIN FOLDING IN THE ACT: RESOLVING (UN) FOLDING TRANSITION PATHS USING ADVANCED SINGLE-MOLECULE SPECTROSCOPY.  Nivin Mothi , Mourad Sadqi, Victor Munoz 826-plat 9:15 am DIRECT DETECTION OF INTRAMOLECULAR DYNAMICS OF MEMBRANE PROTEINS USING TIME-RESOLVED X-RAY SINGLE-MOLECULE TRACKING.  Kazuhiro Mio , Shoko Fujimura, Masaki Ishihara, Muneyo Mio, Masahiro Kuramochi, Hiroshi Sekiguchi, Tai Kubo, Yuji C. Sasaki 827-plat 9:30 am A TUG-OF-WAR MECHANISM DRIVES THE ALLOSTERIC ACTIVATION OF PROTEIN KINASE A.  Lihui Bai, Jeneffer P. England, Rodrigo A. Maillard 828-plat 9:45 am Travel Awardee THE EVOLUTIONARY BIOPHYSICS OF A FORCE-CONVEYING PROTEIN COMPLEX REQUIRED FOR VERTEBRATE HEARING.  Collin Nisler , Yoshie Narui, Vincent Lynch, Marcos M. Sotomayor 829-plat 10:00 am CONFORMATIONAL DYNAMICS OF THE T-CELL RECEPTOR CHASSIS CO- ORDINATES CDR3 LOOP POSITIONING DURING MECHANOSENSING OF PMHC LIGANDS. Wonmuk Hwang , Robert J. Mallis, Matthew J. Lang, Ellis L. Reinherz Exhibitor Presentation Beckman Coulter Life Sciences 8:30 am - 10:00 am, Room 33C Get the High-Resolution Separation That You Have Been Searching for with Preparative and Analytical Ultracentrifugation Introduction: Purification of biological products, including biotherapeu- tics, involves the separation of cells from the culture media, followed by extensive processing to isolate the target of interest. Relatively simple separations are often achieved via differential centrifugation (pelleting), though high-resolution separations often utilize density gradient ultra- centrifugation to yield high purity. In this presentation, we will discuss the full gamut of preparative (ultra)centrifugation, which permits the isolation and purification of biological components ranging from small peptides and nanoparticles to large nucleic acids, viruses, and organ- elles. We will then discuss the analytical/characterization aspects of ultracentrifugation, which allow quantitation of size, mass, shape, and density of the biological components that have been purified, along with exploration of their thermodynamic properties and binding interac- tions. Modern examples will be discussed for both preparative and analytical ultracentrifugation. Purification: Modern centrifuges reach incredibly high speeds (with centrifugal acceleration sometimes exceeding 1,000,000 x g ) to aid the high-resolution separation of particles, typically in the micro- or nanometer range, by size and/or density. Today’s gene therapy prod- ucts, such as viral vectors, require high-quality purification to ensure

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