Biophysical Society 2020 Annual Meeting

the consistent production of safe, efficacious therapeutics of the highest quality to further advance this rapidly growing field and deliver solu- tions to patients in need. Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty cap- sids), along with other materials (such as plasmid DNA) in gene therapy production workflows. Though a well-established and mature method, DGUC is sometimes viewed as dated, challenging to design and conduct, or only suited for small-scale research applications. In this workshop, we’ll address these perceptions and discuss the premise of DGUC as a modern, high-resolution purification technique for AAVs and plasmid DNA. We’ll also provide guidance on how to get started with DGUC and optimize this technique for gene therapy workflows.  Characterization: Analytical ultracentrifugation (AUC) is one of the most versatile biophysical tools used today for the characterization of biologi- cal samples ranging from small drug molecules to intact viruses, vesicles and microparticles. AUC works with biological samples in the native state and does not depend on a reporter species or custom-coated substrates. AUC separates biomolecules based upon both molecular mass and anisotropy and can also be used to quantify interactions between differ- ent species. In this talk, we will start with the principles of AUC and take a tour through the technology behind modern AUC, including detection methods. We then look at advancements of the latest gen Optima AUC. Next, we go through experiment design – including the use of simulation tools. Following, we will address the different types of AUC experiments (equilibrium and velocity), compare and contrast their merits with sam- ple data, and touch upon the principles of data processing. Finally, we will explore a variety of applications with a focus on the unique advan- tages that AUC brings to the study of various biotherapeutics, polymers, nanoparticles, and others – and how AUC compares to and complements other analytical techniques. Speakers Ross VerHeul, Senior Applications Scientist, Beckman Coulter Life Sciences Akash Bhattacharya, Senior Applications Engineer, Beckman Coulter Life Sciences CPOW Committee Meeting From Single Molecules to Tissues – A New AFM Toolkit for Nanoscopic Investigation of Mechanics, Structures, and Dynamic Processes in Life Science The ability of atomic force microscopy (AFM) to obtain three-dimensional topography images of biological molecules and complexes with nanome- ter resolution and under near-physiological conditions remains un- matched by other imaging techniques. JPK BioAFM has developed a new NanoWizard® 4 XP AFMwhich not only enables the high-speed study of the time-resolved dynamics associated with cellular processes, it’s latest scanner technologies and compact design also allow full integration of AFM into advanced commercially available light microscopy techniques. This seminar will focus on how the advances in Bruker’s latest BioAFM can be applied to study a wide-range of biological samples, from indi- vidual biomolecules to mammalian cells and tissues in real-time, in-situ experiments. We will present examples of how we are able to resolve the nanoscale structure of individual biomolecules at high-speed scan rates (150 Hz), follow the dynamic reorganization of the membrane-associated cytoskeleton of living cells at high-temporal and high-spatial resolution, and automatically map the topography of cell cultures across the 8:30 am - 10:30 am, Room 30D Exhibitor Presentation Bruker Corporation 9:30 am - 11:00 am, Room 33A

entire area of the microscope stage. We will also discuss the full suite of BioAFMmodes and accessories for studying the nanomechanical proper- ties of cells and tissues, including direct correlation of multiparametric, quantitative AFM and super-resolution (STED) datasets. Speaker Andrea Slade, BioAFM Product Manager, Bruker Corporation Career Development Center Workshop Demystifying the Academic Job Search II: Preparing your Written Application Materials: CV, Cover Letter, and Research Statement 10:00 am - 11:00 am, Room 26A Over 90% of the cuts in a typical academic job search are made on the basis of your written application materials. Given the large number of candidates in a typical applicant pool, your documents must convey the most important information about you in the most clear and efficient manner. Learn about how your materials should differ based on the type of institution and/or program, and how to create “glance-able” docu- ments to speak most effectively on your behalf. Exhibits 10:00 am - 5:00 pm, Exhibit Hall Coffee Break 10:15 am - 11:00 am, Exhibit Hall Calling all new BPS members! Come and mingle with BPS Staff, Soci- ety Council, and programmembers as you learn about the Society’s activities. Current members are welcome to come and meet with new members. Exhibitor Presentation Bruker Corporation 10:30 am - 12:00 pm, Room 33C Using NMR (Nuclear Magnetic Resonance) and EPR (Electron Paramagnetic Resonance) in Biophysics Magnet Resonance offers many insights into how biological systems func- tion. The two techniques shed light on the identity of species, dynamics, and structures of proteins, peptides, nucleotides, and lipids. The speakers will present an overview of these techniques and applications for people who may be new to the field and wish to incorporate them in their stud- ies. NMR has long been a valuable tool for the determination of structures, the study of dynamic processes and the investigation of interactions in biological molecules. To conduct these studies on larger molecules higher magnetic fields are required. Bruker BioSpin has successfully installed a 1.1 GHz NMR system in a customer laboratory and the delivery of the first 1.2 GHz system is imminent. To complement the higher magnetic fields Bruker Biospin has also introduced several new probes for liquid and solid state NMR. NMR has recently been used successfully for the characterization of large proteins such as monoclonal antibodies. The statistical analysis of NMR spectra allows the detection of changes in the high order structure of these molecules. Meet the Editors, The Biophysicist 10:15 am - 11:00 am, Society Booth/Lobby G New Member Welcome Coffee 10:15 am - 11:15 am, Room 28CDE

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