Biophysical Society 59th Annual Meeting Program Guide

8:00 am –6:30 pm , R oom 301/302/303 Career Center 8:00 am –10:00 pm , H all C Poster Viewing 8:15 am –10:15 am , B allroom I Symposium Regulated Protein Bridges Connecting Membranes: STIM Proteins in Cellular Signaling Chair Richard Lewis, Stanford University 52-S ymp 8:15 am SINGLE-MOLECULE STUDIES OF THE ER CALCIUM SENSOR STIM1. Richard Lewis 53-S ymp 8:45 am TUNING THE TAPS: STIM1 AND STIM2 REGULATORY MECHANISMS. Barbara A. Niemeyer 54-S ymp 9:15 am GATING MECHANISMS OF STORE-OPERATED CRAC CHANNELS. Murali Prakriya 55-S ymp 9:45 am THE MECHANICS OF STIM-ORAI COMMUNICATION. Patrick Hogan

Sunday, February 8 7:30 am –8:30 am , R oom 327/328/329 Postdoctoral Breakfast

Supported by the Burroughs Wellcome Fund

This breakfast presents an opportunity for postdoctoral members of the Society to meet and discuss the issues they face in their current career stage. This year, there will be an emphasis on challenges and opportunities in publishing. Members of the Early Careers Committee will be available to answer questions about how the Committee serves postdocs in the biophysical community. Limited to the first 100 attendees. 7:30 am –9:00 am , H all C, R oom A Exhibitor Presentation FEI Company FEI Cryo-TEMWorkflow Solutions: A New Era for 3D Structural Biology A new frontier exists in unraveling interactive biological and biochemical processes and pathways at the macromolecular level. Of critical importance is the three-dimensional visualization of macromolecular structures and molecular machines in their native functional state. Three techniques play a major role in orchestrating this. Nuclear magnetic resonance (NMR) has the capability to study specific protein domains or fragments and their functional role in protein folding and dynamics and in ligand binding whereas X-Ray crystallography (XRD) allows visualizing high-resolution but more static 3D structures of apo and liganded proteins, mainly in a monomeric or dimeric state after crystallization. To unravel more physiologically relevant situations however, it is essential to visualize multimeric complexes in their tertiary and quaternary state and their interaction with other complexes. By performing typical cryo-TEM applications like single particle analysis or tomography, this can be achieved. In this so-called translational methodology, cryo-TEM thus provides complementary information to NMR and XRD that can be crucial for drug discovery, e.g. in terms of a better understanding of the mechanism of action inferred from the EM structure of the physiologically relevant complex. This will eventually contribute to answer real biologically as well as medically relevant questions. Latest developments in the cryo-TEM workflow have brought the 3 major structural biology technologies closer together. Now, finally, a continuum has been reached on all important aspects with regards to resolution and macromolecular scales which allows for the full deployment of the combination of these technologies. Here, we will illustrate the historical context of these technologies with respect to one another and show how latest developments have reached the critical requirements to fully unleash the power of structural biology in not just answering fundamental questions, but actually contribute to curing diseases and improving health. Also, we will discuss the future of structural biology based on the latest developments of the FEI workflow and its components with a special focus on the advances in contrast enhancement (phase plates) and (direct electron) detection. Presenter Chris Arthur, Applications Engineer, FEI Company 7:30 am –5:00 pm , C harles S treet L obby Registration/Exhibitor Registration

8:15 am –10:15 am , B allroom II Symposium Mechanosensors

Chair Marcos Sotomayor, Ohio State University 56-S ymp 8:15 am

THE MINIMAL CADHERIN-CATENIN COMPLEX BINDS TO ACTIN FILAMENTS UNDER FORCE. Craig Buckley, Jiongyi Tan, William Weis, W. James Nelson, Alexander Dunn 57-S ymp 8:45 am MECHANISMS AND MECHANOSENSITIVITY: EXCEPTIONAL CADHERINS FOR HEARING AND BALANCE. Marcos Sotomayor 58-S ymp 9:15 am MECHANICAL FORCES IN B CELL ACTIVATION. Pavel Tolar 59-S ymp 9:45 am NAVIGATING A MAZE - SENSING AND RESPONDING TO MECHANICAL OBSTACLES DURING CELLULAR INVASIVE GROWTH. Carlos Agudelo, Amir Sanati Nezhad, Mahmood Ghanbari, Muthukumaran Packirisamy, Anja Geitmann

8:15 am –10:15 am , B allroom III Platform Molecular Simulation: Structure and Interactions

Co-Chairs Oliver Beckstein, Arizona State University Alexander Shug, Karlsruhe Institute of Technology, Germany

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland