Biophysical Society 60th Annual Meeting

Exhibitor Presentations Rooms 505, 513, Los Angeles Convention Center

Room 505: Sunday, February 28

Room 505: Monday, February 29

9:30 am–11:00 am Sophion together with Biolin Scientific Pioneering Ion Channels - Expanding the Boundaries of Automated Patch Clamp Recent advances in automated patch clamp for voltage and ligand gated ion channels with emphasis on NMDA, cardiac safety and induced pluripotent stem cells Speakers Timm Danker, NMI, Germany Automated Patch Clamp on Crdiac Ion Channels and Multiwell MEA Recordings on Human iPSC-derived Cardiomyocytes: a Complementary Approach for Predictable Proarhytmia Assessments Caterina Virginio, Aptuit NMDA Receptors: Meaningful Biophysical and Pharmacological Studies to Redefine Ligands Properties Denise Franz, University of Rostock, Germany Electrophysiological Characterization of Human Induced Ppluripotent Stem Cell-derived Dopaminergic Neurons on the QPatch 11:30 am–1:00 pm Asylum Research, an Oxford Instruments Company Soft, Sticky, and Viscous: Practical Considerations for Measuring Cell Mechanics with AFM The atomic force microscope (AFM) has found broad use in the investiga- tion of cell mechanics, with numerous studies of cell stiffness and modu- lus dating back over a decade. Because AFM can quantitatively measure the mechanical properties of individual live cells, novel insights to cell function and to cell-substrate interactions have been realized. This is per- tinent for cell biology, as it has been demonstrated that the geometrical and mechanical properties of the extracellular microenvironment are important in such processes as cancer, cardiovascular disease, muscular dystrophy, and even the control of cell life and death. Indeed, the abil- ity to control and quantify these external geometrical and mechanical parameters now arises as a key issue in the field and AFM seems poised to play a prominent role in building that understanding. The use of AFM in this field presents unique challenges and opportuni- ties. Some of the most important considerations are because many of the AFM techniques used here have largely been borrowed from those first developed for materials science. This is simultaneously a success of inter- disciplinary research and an opportunity to further tailor measurements to cells and biological materials, which have some fundamentally differ- ent characteristics compared to polymers. Most dramatically, cells are far “softer” than polymers, usually at least 100× lower in modulus than even soft rubbers and easily 10,000× lower in modulus than some common plastics. Further, cells are usually quite “sticky,” leading to large adhesion to the AFM tip that can complicate measurements. Finally, cells are often strongly viscoelastic, exhibiting not just elastic deformation described by the elastic modulus but also a viscous response that depends on

5:30 pm–7:00 pm HEKA Elektronic + Multi Channel Systems

PATCHMASTER and PatchServer: Solutions for Patch Clamp

Presentation 1: Combined Patch Clamp and Imaging with PATCHMASTER and SmartLUX SmartLUX is the new imaging extension for PATCHMASTER software synchronizing image acquisition and patch clamp data recordings. Image based data such as fluorescence intensities form ROIs that are stored as traces together with current and voltage traces in the PATCHMASTER data file. A link between data points of the trace and the images enables convenient automatic display of the corresponding images when replay- ing the patch clamp data. Presenation 2: Multi-Patch Experiments with EPC 10 Quadro and PATCHMASTER PATCHMASTER software allows the user to control up to 8 patch clamp amplifiers (2 x EPC 10 USB Quardo) in parallel, making it an ideal platform for either conventional multi-patch experiments or automated patch clamping. The Multi-Cell extension of PATCHMASTER allows easy setup and execution of acquisition sequences and analysis methods for operat- ing all amplifiers in parallel. Conventional patch clamping with multiple electrodes can be facilitated by automating processes using the Protocol Editor. Presentation 3: PatchServer: A Pipette-Based Automatic Patch Clamp System PatchServer is Multi Channel Systems’ new automated patch-clamp sys- tem that adds on to a manual patch-clamp setup. It is able to establish single-channel and whole-cell recording configurations using standard glass electrodes. The automation includes sealing on suspended cells, establishing recording configurations, and moving to application bays for solution exchange – all under visual control. PatchServer comes in a one channel version for performing single experiments, as well as a four channel version for recording from four cells in parallel using the EPC 10 Quadro from HEKA. A piezo-driven ultra-fast solution exchanger (UFA tool) is available as an option and can be easily integrated. Speakers Christian Heinemann, Head of Engineering at HEKA Elektronik Juergen Rettinger, Product Manager – Ion Channel Product Line at Multi Channel Systems

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