Biophysical Society 60th Annual Meeting

Room 505: Tuesday, March 1

Room 513: Sunday, February 28

11:30 am–1:00 pm Bruker Nano Surfaces BioScope Resolve BioAFM – Unrivalled AFM Biomechanics and Resolution In this presentation we will introduce new capabilities for cell mechanobi- ology and highest resolution cell and molecular imaging available on the BioScope Resolve BioAFM. We will explain how innovations in force con- trol and instrument design have enabled BioScope Resolve to be the first AFM to image microvilli on live cells and to consistently resolve the double helix of DNA, while on the inverted microscope. To enable highest resolu- tion and accurate cell mechanics data, the entire AFMmechanical loop of BioScope Resolve has been designed for stability, specifically on biologi- cal samples, with biological sample carriers, and when mounted on the inverted microscope. Designed for Bruker’s exclusive PeakForce Tapping, BioScope Resolve eliminates the need for fluid cantilever tuning entirely with ScanAsyst and provides quantifiable pN force control for imaging and force mapping. The combination of PeakForce Tapping and FASTForce Volume provides the broadest range of frequencies for mechanical characterization, with a new no-touch calibration method guarantee- ing accurate calibration. As we will show in the presentation, BioScope Resolve also features synchronization of these unique mechanical mea- surements with fluorescence, enabling new kinds of correlative studies. Speaker Marcin Walkiewicz, PhD, Applications Scientist, Bruker – Atomic Force Microscopy Business

10:30 am–12:00 pm Carl Zeiss Microscopy LLC Technology Innovations: ZEISS LSM 880 Confocal with Airyscan and ZEISS Lightsheet Z.1 These microscopes from ZEISS address both ends of the spectrum of sam- ples, live high speed imaging with superresolution and high speed imaging of large live and fixed tissues. Learn how ZEISS LSM 880 with Airyscan maintains the mantra that each photon of emission light is precious, while expanding the triangle of sensitivity, resolution and speed of acquisition. ZEISS LSM 880 with Airyscan allows you to use multicolor samples with any label and get image quality like you have never seen before. With Airyscan you are always able to select the optimal acquisition strategy for your sample: Simply decide whether you want to gain 1.7x higher resolu- tion in all three dimensions – resulting in a 5x smaller confocal volume. Or push the sensitivity beyond the limits of all conventional confocal microscopes; or use the increase in signal-to-noise ratio to speed up your image acquisition. Traditionally, deeply imaging into intact tissue typically requires multi- photon excitation to penetrate deeper than near the surface of a tis- sue. Using a “clearing” method to remove the light obstructing opaque molecules from a tissue has been another technique for deep imaging. Techniques such as SCALE, CLARITY, ClearT, SeeDB, CUBIC and others have allowed researchers to image deeper than a millimeter into cleared animal model brains and organs. ZEISS Lightsheet Z.1 features high speed image acquisition and greatly reduced photo damage making imaging of live developmental samples and fixed and cleared tissues easier than ever before. Come learn about using the innovative ZEISS Lightsheet Z.1 microscope for imaging of fixed and cleared tissues. Speaker Joseph Huff, Product Marking Manager, Laser Scanning and Superresolution Microscopy, Carl Zeiss Microscopy LLC Scott Olenych, Product Marketing Manager, Imaging Products, Carl Zeiss Microscopy LLC

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