Biophysical Society 60th Annual Meeting

Pay Less. Perform Better. Quality Guaranteed. Cal-520™, the Best Green Ca 2+ Indicator

CalciumDye Cal-520 ™ AM

AAT Bioquest

Sigma †

Invitrogen † not available not available

$295/mg ($148/mg ‡ ) $245/mg ($123/mg ‡ )

not available not available not available

Fluo-8® AM Fluo-4 AM Fluo-3 AM

$412/mg ($206/0.5 mg)

$145/mg ($73/mg ‡ )

$452/mg

$254/mg

† Prices are based on the websites of Sigma-Aldrich and Invitrogen as of December 2015 respectively, which may vary for different customers. ‡ Special 50% introductory discount applied (Discount Code: DC50, valid until 12/31/2016).

ATP/μM ( with probenecid)

ATP/μM ( without probenecid)

ATP-stimulated calcium responses of endogenous P 2 respectively, with or without probenecid under the same conditions.

Y receptors in CHO-K1 cells incubated with Cal-520™ AM or Fluo-4 AM

Sensitive

Enabling difficult Ca 2+ assays with the highest signal/background ratio.

Robust

Compatible with probenecid-sensitive cell lines & challenging targets.

Convenient

Essentially identical spectra to those of Fluo-4 and Fluo-8® .

Product Ordering Information

Cat # 21131 20512 20532 20550 21080

Product Name Cal-520™ AM Cal-590™ AM Cal-630™ AM

Size 1 mg 1 mg 1 mg 1 mg 1 mg

Ex (nm)

Em (nm)

K

(nM)

d

492 573 608 494 494

514 588 626 516 517

320 561 792 345 389

Fluo-4 AM Fluo-8® AM

Please visit us at booth #804 For more information about Cal-520™ calcium indicators Call 1-800-990-8053 or visit our website www.aatbio.com

AAT Bioquest ® Advancing Assay & Test Technologies