Biophysical Society 61st Annual Meeting | Program Guide

Room 218: Tuesday, February 14 11:30 am–1:00 pm Bruker Corporation BioScope Resolve - The Highest Resolution Imaging of Any BioAFM The BioScope Resolve™ BioAFM provides the highest resolution imag- ing, most complete biomechanics capabilities, and fastest scanning of any bioAFM available. Specifically designed for integration onto inverted optical light and confocal microscopes, it enables investigation of a wide range of biological samples, from cells and tissues to molecular and pro- tein structures. Our exhibitor presentation will talk about three aspects of the Bioscope Resolve BioAFM system that are advancing biological research around the world. 1. HighResolutionImaging-Theuniquemechanicalstabilityandperfor- manceoftheBioScopeResolveAFMonanopticalmicroscopeenables researchers to routinely resolve the major and minor grooves along the backbone of an individual DNA strand. With ScanAsyst®, high- resolution cell imaging has never been easier. This game-changing capability has allowed soft, flexible cell surface structures, such as microvilli, to be resolved for the first time on living cells by AFM. 2. Complete Cell Mechanics Data – Accurate, Repeatable. PeakForce QNM®, has become the preferred mode for nanomechanical mapping. With new algorithms, it delivers highest resolution property maps at pN forces on live cells. Together with “No Touch” tip calibration, PeakForce QNM delivers the fastest quan- titative mechanical characterization of live cells, providing a com- plete force curve for every pixel at actuation rates from 125 Hz to 2 kHz. With our new FASTForce Volume covering rates from sub-Hz to 300 Hz, and PeakForce Tapping extending to 2 kHz, we provide the widest range of ramp rates for single points and imaging while maintaining pN force control. Our new RampScript™ allows the user to build, control and record com- plex nanomechanical measurements for use in protein pulling, ligand-receptor interaction, cell relaxation and viscoelastic probing. 3. Investigating Cell Dynamics – Without Compromise. Fast Tapping on living cells - Bioscope Resolve is the only BioAFM that enables fast scanning while simultaneously providing the large piezo scan- ner range necessary for capturing the dynamic behavior of living cells in real time (XYZ = 100µm x 100µm x 15µm). The Fast Tapping capabilities of Resolve are truly a step forward in conducting high- resolution AFM studies at timescales relevant to cellular processes. Together with the integration of advanced optical microscopy and environmental control, live cell studies have never been easier. BioScope Resolve was designed by biologists who needed a flexible BioAFM system that could meet a wide range of biological application needs. From its Advanced Perfusing Incubator for long-term live-cell studies to its full sample-carrier support system, every feature of the BioScope Resolve is designed for maximum experiment flexibility. Speaker Ian Armstrong, Applications Scientist, ARM Unit, Bruker Corporation

5:30 pm–7:00 pm Sutter Instrument Scientists Empowering Scientists

Patch clamp electrophysiology, which started as a highly specialized technique, is now considered a primary tool for biological research. To meet the needs of the research community, Sutter Instrument has introduced a new line of flexible and intuitive patch clamp amplifiers and data acquisition software. The IPA® Amplifier and SutterPatch® Software will allow the researcher to quickly set up and perform routine tasks, but still have the flexibility to meet the demands of the experienced electrophysiologist. At this presentation, we will demonstrate how the IPA Integrated Patch Amplifier, the new Double IPA® Amplifier and SutterPatch Software can be used for a variety of common experiments, including characterization of ionic current and recording synaptic events in tissue slices. We will also show how the IPA family of amplifiers and SutterPatch software can be used in much more complex acquisition and analysis scenarios. Data collection in SutterPatch Software was designed to track environ- mental variables along with the context of each sample within an experi- ment, so that all data are recorded together for future reference. We focused on creating an intuitive navigational interface to make large data sets more manageable. Controls that are already familiar to you, from electrophysiology software or applications in other fields, make finding a specific section of an experiment very easy. Where appropriate, we used innovative new approaches to make the interface as simple and powerful as possible. Paradigms and Routines control the experiment in a way that enables a high degree of automation, helping to eliminate operator bias. Real-time decision making and sophisticated control of the experimental flow are made easy with a comprehensive set of sample parameters. Even com- plex stimulus waveforms can easily be configured using the Waveform Editor or a template created from a recorded or synthesized signal. Who should attend? • Electrophysiologists who use amplifiers, micropipettes and micro- manipulators for patch clamp, sharp electrode or extracellular recordings. • Researchers who perform patch clamp recordings in tissue slices, dissociated cells, cell lines or in vivo preparations. • Anybody who is interested in the latest feature-rich hardware and software for electrophysiology applications. Speakers Jan Dolzer, Product Manager, Patch Clamp Systems, Sutter Instrument Telly Galiatsatos, Tech Support and Product Development, Sutter Instrument

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