Biophysical Society 61st Annual Meeting | Program Guide

6:30 pm–8:00 pm Molecular Devices LLC Getting the Most Out of Your Experiments with pCLAMP and HumSilencer Technology

10:30 am–12:00 noon Beckman Coulter Life Sciences What Goes Around, Comes Around: Unveiling the Optima AUC For more than 70 years, Analytical Ultracentrifugation (AUC) has played a critical role in laying the foundations for modern molecular biology. AUC allows for the characterization of proteins, oligomers, aggregates, particles, colloids, nanoparticles, extracellular vesicles and other small structures in native conditions and matrix-free conditions. By measuring proteins and other macro-molecules as interacting elements instead of in isolation, AUC more closely approximates true physiological conditions offering unique insight into molecular characterization and processes including molecular weight determination/conformation, stoichiometry, heterogeneity as well as thermodynamic parameters associated with binding. Following a brief overview of the fundamentals of AUC operation and analysis, the talk will include a brief introduction of the new Optima AUC system. Comparative data from the new Optima AUC versus the ProteomeLab series will be presented, while highlighting applications that are now enabled in the new system due to multi-wavelength analysis and faster scan speeds. Analytical Ultracentrifugation is a complementary technique to many oth- ers; the talk will illuminate advantages of AUC over existing techniques for different applications. Lastly, future research fields enabled by the new Optima AUC will be discussed. Speaker Chad Schwartz, AUC Product Manager, Beckman Coulter Life Sciences

The patch-clamp technique remains the best method for evaluating ion channel physiology, and since 1983 Axon Instruments has been the gold standard in patch-clamp equipment. Axon Instruments continues to push the envelope with new innovations with best-in-class systems and software. Join this workshop to learn about our latest breakthrough, HumSilencer technology, built into the Digidata 1550B Data Acquision System. HumSilencer is a new and easy way to eliminate 50 or 60 Hz line synchro- nous noise and associated high frequency harmonics WITHOUT the use of a filter. In addition to HumSilencer, pCLAMP remains the industry standard soft- ware package for electrophysiology because of its power, flexibility and ease of use. Are you getting the most out of your pCLAMP software? Join this workshop to learn more about how you can maximize what you can do with pCLAMP and learn something you may not know. Speaker JeffreyWebber, Product Manager, Electrophysiology, Molecular Devices LLC Room 221: Monday, February 13 Eight out of the top ten drugs worldwide in 2016 are biopharmaceuticals. Microcalorimetry is a powerful tool in the characterization of their struc- ture and stability. Isothermal titration calorimetry (ITC) has long been the gold standard technique for exploring the binding of any drug to its target molecule. Recent publications describing the advantages of enthalpy screening utilizing the advances in ITC hardware and software exempli- fied by the Affinity ITC instruments from TA Instruments have shown that microcalorimetry remains a powerful tool in characterizing biopharma- ceuticals. Isothermal Calorimetry (IMC) has the sensitivity and flexibility to fully characterize formulation stability and aggregation kinetics of even the high concentration (200-300mg/mL) biopharmaceutical formulations. The sensitivity and flexibility of the TAM IV microcalorimetry system has shown the ability to rapidly characterize even the slowest degradation or aggregation reactions taking place in these biopharmaceutical formula- tions without diluting the sample. Attendees will participate in a wide ranging discussion of these powerful techniques including a full detailed description of the new Affinity ITC and TAM IV hardware along with a Q&A session focused on experimental design considerations for success- fully performing enthalpy screening and biopharmaceutical formulation stability testing. Join your colleagues in this in-depth discussion of how ITC and IMC can speed up the candidate selection process and benefit overall lab productivity in any drug discovery or development effort. Speaker Dile Holton, Microcalorimetry Product Manager, TA Instruments 8:30 am–10:00 am TA Instruments Instrumentation and Experimental Design for Utilization of ITC and IMC Techniques for Characterization of Biopharma- ceuticals

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