Biophysical Society 62nd Annual Meeting | Program Guide

5:30 PM–7:00 PM Sutter Instrument Scientists Empowering Scientists

1:30 PM–3:00 PM Journal of General Physiology

Journal of General Physiology : Celebrating 100 Years The Journal of General Physiology has published seminal biophysical discoveries since 1918 and continues to disseminate mechanistic and quantitative physiology of the highest quality. Join us in celebrating 100 years of JGP during this special presentation featuring the editors and distinguished guests. A full program will be available at the JGP booth #219 in the Exhibit Hall. Speakers Sharona Gordon, Editor-in-Chief, Journal of General Physiology Richard Aldrich, Associate Editor, Journal of General Physiology José Faraldo-Gómez, Associate Editor, Journal of General Physiology Henk Granzier, Associate Editor, Journal of General Physiology Merritt Maduke, Associate Editor, Journal of General Physiology Eduardo Rios, Associate Editor, Journal of General Physiology Kenton Swartz, Associate Editor, Journal of General Physiology 3:30 PM–5:00 PM KinTek Corporation Using KinTek Explorer Software to Understand Kinetics and Rigorously Fit Data In this presentation, Dr. Johnson will introduce the theory and opera- tion of KinTek Explorer software to show how easy it is to fit data to any user-defined model without resorting to the use of equations. Examples of experiments that can be fit include: transient and single turnover stopped-flow kinetics, steady state kinetics, slow onset inhibi- tion, equilibrium titrations, rapid-quench-flow kinetics, temperature dependence, and voltage-dependent rate constants. In addition, time- resolved absorbance or fluorescence and pH-dependent spectra can be analyzed by singular value decomposition to yield spectra and time- or pH-dependence of each species. Fast dynamic simulation using propri- etary algorithms for numerical integration allows you to explore param- eter space and learn kinetics. By modeling the experiments exactly as performed, all details of the experimental setup are included, eliminat- ing errors in interpretation. Moreover, multiple experiments can be fit simultaneously to a single unifying model. Only KinTek Explorer offers such robust and dynamic data fitting. In addition to describing KinTek Explorer’s basic features, Dr. Johnson will introduce new features and will be available to help you to fit your own data. Learn about what you are missing in your own data fitting. See www.kintekcorp.com for more information. Speaker Kenneth Johnson, Professor of Biochemistry, University of Texas at Austin, President, KinTek Corporation

There have been many technological evolutions in Patch Clamp electro- physiology over the past 4.5 decades that Sutter Instrument has been collaborating with researchers. During this period, Sutter has intro- duced many new product families, including pipette pullers, manipula- tors, light sources, wavelength switchers, specialized microscopes, and most recently, fully integrated patch clamp amplifier systems. At this presentation, we will teach techniques, tips and tricks, and showcase features from three of our product families: pullers, manipulators, and patch clamp systems. Since Sutter Instrument’s inception in 1974, our pipette pullers have been used in a large number of research facilities all over the world. They are considered the unparalleled leader in performance and reli- ability. We will demonstrate how to make the unique micropipettes needed for your application, with a discussion on scoring and cutting, bending, polishing, and beveling. The IPA®, Double IPA® and new dPatch® Ultra-fast, Low-noise Integrated Patch Clamp Amplifiers, and SutterPatch® Software can be used for a variety of common experiments, including characterization of ionic current and recording synaptic events in tissue slices. We will demonstrate how the SutterPatch Software’s online measurements and sophisticated control of experimental workflow can be used to aid real-time decision-making and eventually simplify analysis. Sutter introduced Micromanipulators in 1985. From that time on, the company has continued to develop manipulators with stepper motor drive mechanisms and ergonomic controllers that are adaptable to mandifferent experimental designs and platforms. We will introduce two newer additions to the product family: the four axis QUAD® and the three-axis TRIO®. In addition, we will demonstrate how the Multi- Link™ software can be used for robotic control and integration with other hardware. Registration is available online through the Sutter Instrument Event Registration page https://sutter.eventbrite.com. Speakers Adair Oesterle, Product Manager, Micropipette Pullers, Sutter Instrument Geoff Lambright, Product Manager, Microscopy, Sutter Instrument Telly Galiatsatos, Tech Support and Product Development, Sutter Instrument Jan Dolzer, Product Manager, Patch Clamp Systems, Sutter Instrument

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