Biophysical Society 62nd Annual Meeting | Program Guide

Room 5: Monday, February 19

11:30 AM–1:00 PM Asylum Research, an Oxford Instruments Company High Resolution and High Speed Imaging Innovations and Advancements for Visualizing Dynamic Processes at the Nanoscale Asylum Research will share the latest results from the Cypher VRS, the world's first and only full-featured video-rate AFM. Until now, this capability was only available on AFMs built solely for video rate imaging with limited capabilities such as sample size. The Cypher VRS enables high quality imaging at over 625 lines per second, corresponding to about 10 frames per second. This speed greatly exceeds other “fast scanning” AFMs, by a factor of at least 5-10X. The Cypher VRS also features the full range of modes and accessories supported with its environmental scanner, including heating and cooling. These capabili- ties make the Cypher VRS ideally suited for visualizing dynamic bio- molecular processes at the nanoscale. Additionally, Andor will present their SRRF-Stream, offering the capability to adapt conventional fluo- rescence microscopes to perform live cell superresolution using a large field of view and in real time. SRRF-Stream processes data at up to 30x faster than the corresponding ImageJ post processing implementation of SRRF (Nano-J SRRF). This furthermore permits image acquisition and SRRF processing to happen in parallel, resulting in a massive overall workflow improvement. SRRF-Stream facilitates use of low excitation intensities (mW-W/cm2), prolonging live cell observations and enabling accurate physiology. It is also compatible with conventional fluoro- phores, e.g. GFP, simple labelling, no photo-switching required. By enabling real-time superresolution with large field of view images, the combination of SRRF-Stream and our iXon EMCCD cameras represents a highly cost-effective way to unlock powerful super-resolution from conventional fluorescence microscopes. SRRF-Stream is ideally suited to iXon Life, highly cost-effective single photon sensitive EMCCD camer- as that are streamlined specifically for fluorescence microscopy usage. Finally, Bitplane will present Imaris, its 3D/4D image visualization and analysis software. Imaris interactively renders data sets 100s of GBs to TB in size and with thousands of time points. In addition, Imaris offers a variety of analysis tools – each of them presented in an easy to use wizard. With Imaris 9 the Surfaces tool analyzes extremely large images to report spatial, morphological, and intensity measurements for the characterization of biological objects of all sizes and shapes. Imaris’ multiple tracking algorithms are easily applied to Surfaces to analyze temporal changes and report motion behavior. In addition, the XT module provides a two-way interface from Imaris to classic program- ming languages: Matlab, Java, or Python and an image export/import to Fiji. These features enable Imaris to provide a flexible and powerful solution for the analysis of 3D/4D images. Speakers Sophia Hohlbauch, Applications Scientist, Asylum Research, an Oxford Instruments Company

9:30 AM–11:00 AM Bruker Corporation The Latest in Mechanobiology Research with AFM Mechanobiology-related research is focused on understanding how cells exert and respond to forces. Examining the effects of forces on cells has a wide-range of applications from understanding disease pathology to the development of tissue engineering devices. Recent advances in atomic force microscopy (AFM) are not only allowing direct observation of cell membrane structures, such as microvilli, on living cells, they are also providing unique opportunities to measure the nanomechanical properties of individual cells, map the spatial distribu- tion of membrane receptors, as well as study the dynamics of various cellular processes and behaviors. In this session we will introduce the newest advancements in AFM technology designed to enable quantitative nanomechanical property research at the cellular and molecular levels. Come see how research- ers can look at, map, and measure mechanical properties like the adhe- sion forces between cells and molecules, and visualize their dynamic behaviors, as well as capture high-resolution images. Before you come, check out these leading researcher interviews, talk- ing about their current work using AFM: • Using AFM to study cell mechanics and function: https://www. news-medical.net/news/20171017/Using-AFM-to-study-cell- mechanics-and-function.aspx • Probing living cells with AFM: https://www.news-medical.net/ news/20171121/Probing-living-cells-with-AFM.aspx • Using AFM to study cancer cells: https://www.news-medical.net/ news/20171114/Using-AFM-to-study-cancer-cells.aspx • Studying the nanomechanical properties of aging and cancerous cells using AFM: https://www.news-medical.net/news/20171031/ Studying-the-nanomechanical-properties-of-aging-and-cancerous- cells-using-AFM.aspx • Measuring biological samples using SNAP: https://www.news- medical.net/news/20171024/Measuring-biological-samples-using- SNAP.aspx Speaker Ian Armstrong, Sales Applications Manager, Bruker Corporation

Colin Coates, Product Manager, Andor Technology Chi-Li Chiu, Technical Support Specialist, Bitplane

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