Biophysical Society 62nd Annual Meeting | Program Guide

3:30 PM–5:00 PM Wyatt Technology Corporation

5:30 PM–7:00 PM LUMICKS BV

Light Scattering Tools for Biophysical Characterization Explore Wyatt Technology’s powerful suite of light scattering tools for biophysical characterization of protein and other biopolymer samples. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) experiments help quantifying many critical attributes of samples, such as their molar mass, radius, and degree of conjugation. At the same time, these techniques allow characterization of sample preparation quality by giving information about the aggregate content, thermal sta- bility, and details of self- and hetero-association. All these parameters may not be amenable to standard characterization methodology but are readily and consistently elucidated with light scattering. Due to their ease of use, potential for automation and high throughput capabilities, light scattering techniques can be incorporated into many workflows, such as a quality control tool prior to surface plasmon reso- nance (SPR), biolayer interferometry (BLI), and isothermal titration calo- rimetry (ITC) experiments. Light scattering can also be used to select samples for further characterization in large scale instrumentation, like small angle X-ray scattering (SAXS) or small angle neutron scattering experiments (SANS), and thus help in utilizing expensive large scale instrumentation more efficiently. The high throughput light scattering instrumentation can further be used to screen crystallization trials This seminar will review static and dynamic light scattering theory and instrumentation, and then discuss a set of complementary techniques, all based on light scattering, that are useful in addressing many sample characterization aspects. Speaker Andre Mueller, Application Scientist, Wyatt Technology Corporation

Novel Developments and Applications of Single-Molecule Tools with Ultra-High Resolution, Stability, and Through- put LUMICKS brings to market revolutionary single-molecule technologies that enable – for the first time –visualization of molecular interac- tions and acoustic manipulation of biomolecules. We aim at creat- ing an environment for researchers to perform high quality, high throughput single-molecule, and cell experiments, in the most acces- sible manner by providing novel single-molecule instruments. During this seminar, we will discuss the latest developments and applications of our single-molecule technologies and how they can enhance the understanding in the fields of DNA/RNA-protein interac- tions and kinetics, molecular motors, protein folding, genome organi- zation, membrane dynamics, and much more. The C-Trap™ is the world’s first instrument to combine high-reso- lution optical tweezers, confocal microscopy or STED nanoscopy, and an advanced microfluidics systems in a truly integrated and cor- related solution. This allows scientists to simultaneously manipulate and visualize molecular interactions in real-time. Acoustic Force Spectroscopy (AFS™) is LUMICKS’ highly parallel single-molecule manipulation method, capable of applying forces on thousands of biomolecules in parallel with high precision. Our technologies are designed for easy and automated user interface, with high-through- put capabilities and world-wide technical support. Speakers Rosalie P.C. Driessen, Applications Scientist, LUMICKS BV Ali Raja, Sales Manager, LUMICKS BV Avin Ramaiya, Technology and Application Development Scientist, LUMICKS BV Jordi Cabanas-Danés, Application Scientist, LUMICKS BV Arne Gennerich, Associate Professor, Albert Einstein College of Medicine Willem Peutz, Sales Director, LUMICKS BV

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