Biophysical Society 62nd Annual Meeting | Program Guide

ROOM 6: Monday, February 19 8:30 AM–10:00 AM TA Instruments – Waters LLC Characterizing Biopharmaceuticals for Stability and Affinity We will be discussing native and multi-parameter approaches to testing biopharmaceuticals. Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) are powerful tools for in-depth characterization of molecular binding events and structural stability of biopharmaceuticals. DSC and ITC generate comprehensive thermo- dynamic profiles for protein domain structures and the energetics of inter- and intra-molecular binding events. In addition to these stabil- ity and affinity assays, we have a new technique for determination of longer-term stability. Using an isothermal calorimeter, we can quantify shelf-life stability while simultaneously determining the percent ag- gregated material. This test is typically completed in a few days and has been shown to agree with longer-term SEC data. Speakers Colette Quinn, Microcalorimetry Product Manager, TA Instruments – Waters LLC Malin Suurkuusk, Isothermal Calorimetry Product Manager, TA Instruments – Waters LLC

10:30 AM–12:00 PM Dynamic Biosensors GmbH Biophysical Analysis of Molecular Interactions with the switchSENSE Biosensor switchSENSE is an automated biosensor chip technology employing electrically actuated DNA nanolevers for the real-time measurement of binding kinetics (kON, kOFF) and affinities (KD). Interactions between proteins, DNA/RNA, and small molecules can be detected with femto- molar sensitivity. At the same time, protein diameters (DH) are analyzed with Angstrom accuracy and conformational changes and melting transitions (TM) can be measured using minimal amounts of sample. The principles and applicability of static and dynamic measurement mo- dalities will be introduced in this talk. We will discuss unique possibilities for the functionalization of the sensor surface, e.g., the adjustment of ligand densities and the precise assembly of different ligands on bifunc- tional nanolevers. Application examples from fundamental research and drug develop- ment will be presented, including: • Introduction to the analysis of molecular interactions with electro- switchable DNA nanolevers • Quantification of conformational changes in proteins and Stokes radius measurements • Analysis of complex binders: high-affinity and bispecific antibody formats • CRISPR/Cas9 – nucleic acid interactions and enzymatic activity measurements • Controlling the density of ligands on a chip surface by electrical desorption and “invisibility cloaking” • TUTORIAL: Programming of measurement workflows and data analysis Speakers Ulrich Rant, CEO, Dynamic Biosensors GmbH Kenneth Dickerson, Director of Business Development in North America, Dynamic Biosensors GmbH

Joanna Deek, Scientist, Dynamic Biosensors GmbH Felix Kroener, Scientist, Dynamic Biosensors GmbH Daisylea de Souza Paiva, Technical Sales Manager, Dynamic Biosensors GmbH

191