Biophysical Society 62nd Annual Meeting | Program Guide

12:30 pm–2:00 pm Nanion Technologies GmbH Part One: Ion Channel Analysis – Today’s Contemporary Systems for Safety and Efficacy Screening Nanion provides “smart tools for electrophysiologists.” If you are study- ing ion channels and electrogenic transporters, our chip- and plate- based devices are well suited to advance your research and screening projects. You will find instrumentation for patch clamp, bilayer record- ings, SSM-based electrophysiology, impedance, and extracellular field recording within our portfolio. In our first workshop, we will focus on two plate-based devices for higher throughput assays: The SyncroPatch 384/768PE, an automated patch clamp platform, re- cords from up to 768 cells simultaneously. Application areas range from HTS cardiac safety assessment and efficacy screening to the analysis of ion channel mutations. The SyncroPatch 384/768PE supports volt- age- and current clamp recordings, temperature control, and minimal cell usage. In addition to the use of stably transfected cell lines, more challenging cell assays including stem cell-derived cells, transiently transfected cells, or primary cells can be used successfully. The CardioExcyte 96, a device for label-free analysis of 2D/3D cells/ clusters in a 96 well plate, utilizes two different analysis technolo- gies: Extracellular field potential and impedance. It is a versatile tool for cardiac safety screening given its high resolution which allows the recording of beating iPSC-derived cardiomyocyte networks. The optical lid (CardioExcyte 96 SOL) uses LEDs for pacing cardiomyocytes with light (optogenetics) to study beat rate-dependencies of compounds. Furthermore, long-term impedance measurements of cells over several days makes it an ideal tool for routine toxicity screening (e.g. hepatotox, cardiotox) and cell monitoring. Speakers Andrea Brüggemann, CSO, Nanion Technologies GmbH Niels Fertig, CEO, Nanion Technologies GmbH

2:30 pm–4:00 pm Nanion Technologies GmbH Part Two: Paving the Way for In Depth Pore-, Ion Channel-, and Electrogenic Transporter Analysis In our second workshop we will focus on devices for bilayer record- ings, patch clamp, and electrogenic transporter assays, including live demonstrations. The SURFE 2 R product family enables label-free real time measurement of electrogenic transporter protein activity. Employing SSM (solid sup- ported membrane)-based electrophysiology, the SURFE 2 R instruments compensate for the low turnover rate of these proteins by measure- ment of up to 109 transporters in parallel. This method has proven its value: High quality data on about 100 SLC- and MFS- transporters as well as ATPases and ligand gated ion channels has been published. The flexible single channel instrument, SSURFE 2 R N1, is ideally suited for ba- sic research, whereas the SURFE 2 R 96SE is able to measure 96 sensors in a fully parallel mode enabling larger screening studies on substrates, inhibitors, or modulators. The Port-a-Patch is the world’s smallest patch clamp rig for high quality, giga-ohm seal patch clamp recordings in voltage and current clamp modes. Versatile add-ons, such as internal perfusion, allow unprec- edented experimental freedom, above and beyond the possibilities of conventional patch clamp. The Orbit product family supports parallel lipid bilayer recordings of reconstituted ion channels for four artificial lipid bilayers (Orbit mini) or 16 lipid bilayers (Orbit 16) simultaneously. Using Micro Electrode Cavity Array (MECA, Ionera) recording substrates, the bilayers are automati- cally formed by remotely actuated painting (Ionera- SPREAD), which will be demonstrated during this session. Speakers Andrea Brüggemann, CSO, Nanion Technologies GmbH Niels Fertig, CEO, Nanion Technologies GmbH Maria Barthmes, Product Manager, SURFE 2 R, Nanion Technologies GmbH

Gerhard Baaken, CEO, Ionera Technologies GmbH Ekaterina Zaitseva, CSO, Ionera Technologies GmbH

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