Biophysical Society 62nd Annual Meeting | Program Guide

ROOM 6: Tuesday, February 20

4:30 pm–6:00 pm Bruker Corporation

Harnessing the Power of Superresolution Single Molecule Localization Microscopy with the Vutara 352: Labeling and Imaging Strategies Single molecule localization microscopy (SMLM) has made a significant impact in the field of biology by enabling a 10-fold enhancement in resolution. A key factor in achieving this enhanced resolution is to opti- mally label and image the specimen. Numerous labeling strategies exist to tag structures in cells, bacteria, virus, tissue sections, C. elegans and Drosophila, to make the best use of SMLM. Examples include DNA- and Oligo-Paint, antibody/nanobody labeling with organic dyes, Halo and SNAP-tag dyes, and photo-switchable fluorescent proteins. Choosing a sub-optimal labeling method for a given biological sample will result in loss of achievable resolution. Once a specimen has been optimally labeled and imaged, the acquired localization data can then be readily quantified via statistical analysis to test experimental hypotheses. Join this session to learn about labeling strategies and techniques used to get the best SML results. Speaker Manasa Gudheti, Sales Applications Scientist, Bruker Corporation

10:30 am–12:00 pm Sophion Bioscience A/S

Ion Channel Profiling and Electrophysiological Characteriza- tion Using Automated Patch Clamp (QPatch), Cell Line Gen- eration, and iPSC Derived Cardiomyocytes: Tools for Finding Antibodies and Peptides for Ion Channel Targets Successful ion channel drug discovery requires the integration of mul- tiple technologies and workflows. Sophion Bioscience is a leader in auto- mated patch clamp technology, providing medium to high throughput, automated patch clamp to the pharmaceutical industry and universi- ties. The QPatch is a fully automated patch clamp system, executing simultaneous 8, 16 or 48 parallel patch clamp recordings in conjunction with computer controlled liquid handling, and on-board cell handling. Sophion partners with other biotech companies to create robust, ion channel, and electrophysiological workflows for drug development for ion channel targets. During this workshop, three industry speakers will provide insight into the drug discovery process. Dr. Damian Bell will present how Iontas uses Maxcyte’s scalable electroporation platform and QPatch to advance its antibody programs for ion channel targets. Dr. Daniel Sauter from Sophion Bioscience will present data from the development of protocols for using QPatch with pluripotent stem-cell derived cardiomyocytes from Ncardia (Cor4U). Finally, Dr. Alan Wicken- den from Janssen Research and Development will present on Johnson and Johnson’s development of selective peptide, Nav1.7 inhibitor as a novel analgesic. Speakers Damian Bell, Head of Electrophysiology, Iontas Ltd Daniel Sauter, Application Scientist, Sophion Bioscience A/S Alan Wickenden, Scientific Director and Fellow, Molecular and Cellular Pharmacology, Janssen Research and Development LLC

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