Biophysical Society 62nd Annual Meeting | Program Guide

Public Affairs Committee Meeting 12:15 pm–2:15 pm, South, Level Three, Room 306 International Travel Awardee Luncheon 12:30 pm–1:30 pm, South, Level Three, Room 307/308 A number of international students, postdocs, and scientists will be recognized during this luncheon for their outstanding achievements in biophysics research. This event is hosted by the International Relations Committee. Exhibitor Presentation Alvéole 12:30 pm–2:00 pm, Exhibit Hall, Room 6 Maskless Quantitative Multi-Protein Photopatterning to Orchestrate Cellular Microenvironment Cell biology is faced with significant challenges when attempting to create complex microenvironments to unravel intricate mechanisms in- volved in cell adhesion, cell polarity, cell migration etc. These challenges can be overcome by molecular printing which involves the controlled deposition of molecules on a substrate at the micrometer scale. These approaches have developed tremendously in the past few years and mi- cropatterned substrates are now routinely used for biological research. To yield biologically relevant data, printed biomolecules should mimic the complexity of the in vivo microenvironment. Micrometer-scale gradients of multiple proteins are thus highly desirable. Here we present PRIMO custommicropatterning system for cell control which allows to control the chemistry and topography of the cellular microenvironment and study their impacts on cell development. This maskless quantitative multi-protein photopatterning solution is based on the light-induced molecular adsorption of proteins (LIMAP) technology. The PRIMO system combines a UV illumination module and a specific photoactivatable reagent (PLPP). The combined action of UV- light and PLPP locally degrades antifouling polymer brushes allowing for the adsorption of proteins in a well-defined area. PRIMO relies on a wide-field DMD-based projection system coupled to an epifluorescence microscope to project custom-defined patterns of UV light onto all standard cell culture surface. As a result, micrometer scale patterns are generated within seconds. The remaining background allows for the sequential patterning of multiple proteins. Controlled protein gradients of custom-defined shape can also be patterned. In addition, PRIMO technology allows for microfabrication by photopoly- merization of UV-sensitive materials and also protein patterning onto pre-existing 3D surfaces. This new micropatterning technology empowers biomedical research in neurobiology, immunology, stem cell biology, oncology, and tissue engineering. The applications in cell biology, such as studying how the asymetry of the focal adhesion can regulate the cytoskeleton, will be illustrated by some user testimonials presenting their research works conducted with PRIMO. Visit www.alveolelab.com for more information. Speaker Pierre-Olivier Strale, Senior Scientist, Alvéole

LED illumination and image detectors designed for low magnification the Celldiscoverer 7 achieves new levels of imaging throughput. Complicated tasks of microscope configuration and optimization are completely auto- mated and designed to make the most of any sample type. Automated control of cellular environment allows imaging stability to be maintained over long time course experiments. The system can be expanded with a robotic plate loading system to allow high throughput imaging from plate and slide based samples. The ZEISS LSM 880 confocal with Airyscan and Fast technology offers a unique detector design that counters the typical loss of sample light ex- perienced when using a confocal pinhole. The Airyscan detector provides superresolution down to 120 nm in x,y and 350 nm in z with higher SNR allowing acquisitions with lower laser illumination. The Fast mode for Airyscan provides the ability to image four times faster while maintaining improved resolution and SNR over conventional confocal imaging. The result is gentle superresolution imaging and the needed speed to follow live cells and quantify fast live cell events. Join this workshop and learn how the ZEISS Celldiscoverer 7 and the LSM 880 Airyscan can help your imaging experiments in completely new ways. Speakers Scott Olenych, North american Product Marketing Group Manager, Light Microscopy, Carl Zeiss Microscopy LLC Renée Dalrymple, Product Marketing Manager, Imaging Products, Carl Zeiss Microscopy LLC Colleges in the Community Day 11:30 am–5:00 pm, North, Lower Lobby, Room 20/21 This free day for San Francisco Bay Area college students at the BPS 62nd Annual Meeting kicks off with an Undergraduate Student Pizza “Break- fast” where participants will have an opportunity to network with their peers and members of the Biophysical Society’s Education Committee in a fun and relaxed environment. The Breakfast will include a panel discus- sion on academic and career paths in biophysics, with opportunities for questions and answers from the audience–come prepared to find out about the course of study that aspiring biophysicists undertake, what it means to be a biophysicist, and how biophysicists make important discov- eries. Students will also receive information and advice on how to get the most out of attending the Annual Meeting. Attendees will be permitted to attend any of the meetings open sessions and activities for the full day, including the Graduate & Postdoc Institution Fair where they can meet with representatives of, and learn about, programs from all over the country. Local undergraduate students, and their PI’s, residing within a 50-mile radius of the San Francisco who are not presenting an abstract or listed on an abstract being presented at this meeting may register for this event and gain FREE access to all Annual Meeting sessions on Sunday, February 18, 2018. Pre-registration is required. There will be no onsite registration. Career Development Center Workshop Demystifying the Academic Job Search I: Understanding the Search Process from the Perspective of Search Committees and Decoding Job Announcements 12:00 pm–1:00 pm, South, Lower Level, Room 2 What goes on inside search committees; the “black box” of the academic job search process? How are they constituted, what are their processes, and what do they look for when assessing applicants? Answers to these and other questions presented by Andrew Green, a veteran of the aca- demic job search and numerous search committees.

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