Biophysical Society 62nd Annual Meeting | Program Guide

Symposium Membrane Bending: Mechanisms and Consequenc es 4:00 pm–6:00 pm, North, Lower Lobby, Room 24

house. We will also show how this rich, high-replicate image set is used as input for deep neural networks which generate unified, integrated cell models and label free imaging. Finally, we’ll demonstrate how to navigate our large, high replicate 3D image data sets, revealing the subcellular local- ization of key tagged structures. All of our procedures, tools, and data are shared on our webpage, the Allen Cell Explorer (www.allencell.org), which will be highlighted during the presentation. Speakers Allen Institute for Cell Science team

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Co-Chairs Jeanne Stachowiak, University of Texas, Austin Anne Ulrich, Karlsruhe Institute of Technology, Germany 175-Symp 4:00 pm STOCHASTIC MECHANISMS IN MEMBRANE TRAFFIC.  Jeanne Stachowiak 176-Symp 4:30 pm FLIPPING HELICES: MEMBRANE INSERTION OF amPHIPHILIC HELICES AND EXTRUSION OF TRANSMEMBRANE SEGMENTS.  Torsten Walther, Lena Steger, Erik Strandberg, Ariadna Grau Campistany, Parvesh Wadh- wani, Benjamin Zimpfer, Jochen Bürck, Dirk Windisch, Katharina Becker, Stephan Grage, Johannes Reichert, Sergiy Afonin, Anne S. Ulrich 177-Symp 5:00 pm FRICTION-DRIVEN SCISSION OF MEMBRANE TUBES.  Andrew Callan-Jones 178-Symp 5:30 pm MOLECULAR MECHANISMS OF MEMBRANE REMODELING. Ralf Langen Symposium Channel Mechanisms: Sensing and Gating 4:00 pm–6:00 pm, North, Lower Lobby, Room 25 Co-Chairs MOLECULAR REARRANGEMENTS UNDERLYING FUNCTION OF LARGE CONDUCTANCE CALCIUM- AND VOLTAGE-REGULATED POTASSIUM CHANNELS.  Teresa Giraldez 180-Symp 4:30 pm STRUCTURE AND MECHANISMS OF SELECTIVITY GATING, INHIBITION AND ACTIVATION IN AN ION CHANNEL.  Robert Stroud , Alexander F. Kintzer 181-Symp 5:00 pm INSIGHTS INTO GATING OF GIRK (KIR3) CHANNELS THROUGH G PROTEIN- INDEPENDENT PATHWAYS.  Paul A. Slesinger 182-Symp 5:30 pm CAN K + BE CONDUCTED THROUGH A NARROW PORE? INVESTIGATING THE ROLE OF CONFORMATIONAL CHANGE IN GATING KIR CHANNELS.  Jacqueline M. Gulbis , David M. Miller, Katrina Black, Adam P. Hill, Derek Laver Platform Protein Structure, Prediction, and Design 4:00 pm–6:00 pm, South, Level Two, Room 207/208 Co-Chairs Shruthi Viswanath, University of California, San Francisco Thrasyvoulos Karydis, MIT 183-Plat 4:00 pm ELECTRIC FIELD OPTIMIZATION IN ENZYMES.  Valerie Vaissier 184-Plat 4:15 pm DESIGNED ENZYMES: CREATING A MORE EFFICIENT NITRIC OXIDE DIOXY- GENASE. Mia C. Brown , Kelly Greenland, Lei Zhang, Ronald L. Koder Teresa Giraldez, University of La Laguna, Spain Robert Stroud, University of California, San Diego 179-Symp 4:00 pm

Early Careers Committee Meeting 3:30 pm–5:00 pm, South, Level Three, Room 306 Exhibitor Presentation Wyatt Technology Corporation 3:30 pm–5:00 pm, Exhibit Hall, Room 5

Light Scattering Tools for Biophysical Characterization Explore Wyatt Technology’s powerful suite of light scattering tools for biophysical characterization of protein and other biopolymer samples. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) experiments help quantifying many critical attributes of samples, such as their molar mass, radius, and degree of conjugation. At the same time, these techniques allow characterization of sample preparation quality by giving information about the aggregate content, thermal stability, and details of self- and hetero-association. All these parameters may not be amenable to standard characterization methodology but are readily and consistently elucidated with light scattering. Due to their ease of use, potential for automation, and high throughput capabilities, light scattering techniques can be incorporated into many workflows, such as a quality control tool prior to surface plasmon reso- nance (SPR), biolayer interferometry (BLI), isothermal titration calorim- etry (ITC) experiments. Light scattering can also be used to select samples for further characterization in large scale instrumentation, like small angle X-ray scattering (SAXS) or small angle neutron scattering experiments (SANS), and thus help in utilizing expensive large scale instrumentation more efficiently. The high throughput light scattering instrumentation can further be used to screen crystallization trials. This seminar will review static and dynamic light scattering theory and instrumentation, and then discuss a set of complementary techniques, all based on light scattering, that are useful in addressing many sample characterization aspects. Speaker Andre Mueller, Application Scientist, Wyatt Technology Corporation Career Development Center Workshop Translating Your Credentials: Writing Effective Resumes and Cover Letters and Your LinkedIn Profile 4:00 pm–5:00 pm, South, Lower Level, Room 2 If you are applying to jobs outside academia, employers (even in biotech/ pharma) will typically ask for a resume, rather than a CV; and want to know much more about your collaboration and communication skills than the content of your dissertation or postdoc research. Learn how to craft written application materials and curate your online presence in a way that showcases the skills and capabilities that employers most covet.

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