Biophysical Society 62nd Annual Meeting | Program Guide

1030-Plat 11:15 am A STUDY OF TRANSCRIPTIONAL ACTIVATION BY THE TRANSCRIPTION FACTOR GAL4 IN SACCHAROMYCES CEREVISIAE BY 3D ORBITAL TRACKING AND IN VIVO RNA LABELLING.  Anh Huynh , Micah Buckmiller, Daniel R. Larson, Tineke Lenstra, Matthew L. Ferguson 1031-Plat 11:30 am DESIGNING SINGLE-CELL EXPERIMENTS WITH DISCRETE STOCHASTIC MODELS.  Zachary Fox , Brian Munsky 1032-Plat 11:45 am MORPHOLOGY OF EMBRYONIC EPIDERMIS: AN EMPIRICAL MULTISCALE BIOPHYSICS APPROACH.  Jesse L. Silverberg , Peng Yin 1033-Plat 12:00 pm A PHYSICAL MECHANISM FOR MICRO-VASCULAR ADAPTABILITY.  Shyr-Shea Chang , Kyung In Baek, Chih-Chiang Chang, Andrew Pietersen, Tzung K. Hsiai, Marcus Roper 1034-Plat 12:15 pm TISSUE-SPECIFIC INTERACTIONS AND FUNCTIONAL VERSATILITY OF DRUG TARGETS CHARACTERIZE ADVERSE EFFECTS OF THE DRUGS.  Jihye Hwang 1035-Plat 12:30 pm DISSECTION OF MULTIPLICITY OF THE GPCR MEDIATED SIGNALING.  Jennifer Chen , Yue Pan, Lynn S. Penn, Jun Xi Career Development Center Workshop Networking for Nerds: How to Create Your Dream Career 11:30 am–12:30 pm, South, Lower Level, Room 2 Wanna land your dream job? Get ready to network! Most jobs and other game-changing career opportunities are not advertised, and even if they are, there is usually a short-list of candidates already in mind. So how do you find out about and access the 90% of jobs and other opportunities that are “hidden”? In this workshop, we will focus on proven networking strategies and tactics to identify new opportunities, locate decision-mak- ers within organizations, solidify your reputation and brand in the minds of those who hire, and gain access to hidden jobs and game-changing opportunities. Discover how networking and self-promotion can enable you to land or even create your dream job from scratch! Exhibitor Presentation Asylum Research, an Oxford Instruments Company 11:30 am–1:00 pm, Exhibit Hall, Room 5 Asylum Research will share the latest results from the Cypher VRS, the world’s first and only full-featured video-rate AFM. Until now, this capabil- ity was only available on AFMs built solely for video rate imaging with lim- ited capabilities such as sample size. The Cypher VRS enables high quality imaging at over 625 lines per second, corresponding to about 10 frames per second. This speed greatly exceeds other “fast scanning” AFMs, by a factor of at least 5-10X. The Cypher VRS also features the full range of modes and accessories supported with its environmental scanner, includ- ing heating and cooling. These capabilities make the Cypher VRS ideally suited for visualizing dynamic biomolecular processes at the nanoscale. Additionally, Andor will present their SRRF-Stream, offering the capabil- ity to adapt conventional fluorescence microscopes to perform live cell superresolution using a large field of view and in real time. SRRF-Stream processes data at up to 30x faster than the corresponding ImageJ post processing implementation of SRRF (Nano-J SRRF). This furthermore High Resolution and High Speed Imaging Innovations and Advancements for Visualizing Dynamics at the Nanoscale

permits image acquisition and SRRF processing to happen in parallel, resulting in a massive overall workflow improvement. SRRF-Stream facili- tates use of low excitation intensities (mW-W/cm2), prolonging live cell observations and enabling accurate physiology. It is also compatible with conventional fluorophores, e.g. GFP, simple labelling, no photo-switching required. By enabling real-time superresolution with large field of view images, the combination of SRRF-Stream and our iXon EMCCD cameras represents a highly cost-effective way to unlock powerful superresolu- tion from conventional fluorescence microscopes. SRRF-Stream is ideally suited to iXon Life, highly cost-effective single photon sensitive EMCCD cameras that are streamlined specifically for fluorescence microscopy us- age. Finally, Bitplane will present Imaris, its 3D/4D image visualization and analysis software. Imaris interactively renders data sets 100s of GBs to TB in size and with thousands of time points. In addition, Imaris offers a va- riety of analysis tools – each of them presented in an easy to use wizard. With Imaris 9 the Surfaces tool analyzes extremely large images to report spatial, morphological, and intensity measurements for the characteriza- tion of biological objects of all sizes and shapes. Imaris’ multiple tracking algorithms are easily applied to Surfaces to analyze temporal changes and report motion behavior. In addition, the XT module provides a two-way interface from Imaris to classic programming languages: Matlab, Java, or Python and an image export/import to Fiji. These features enable Imaris to provide a flexible and powerful solution for the analysis of 3D/4D im- ages. Speakers Sophia Hohlbauch, Applications Scientist, Asylum Research, an Oxford Instruments Company Nanion Technologies GmbH 12:30 pm–2:00 pm, Exhibit Hall, Room 6 Part One: Ion Channel Analysis – Today’s Contemporary Systems for Safety and Efficacy Screening Nanion provides “smart tools for electrophysiologists.” If you are studying ion channels and electrogenic transporters, our chip- and plate-based de- vices are well suited to advance your research and screening projects. You will find instrumentation for patch clamp, bilayer recordings, SSM-based electrophysiology, impedance, and extracellular field recording within our portfolio. In our first workshop, we focus on two plate-based devices for higher throughput assays: The SyncroPatch 384/768PE, an automated patch clamp platform, records from up to 768 cells simultaneously. Application areas range from HTS cardiac safety assessment and efficacy screening to the analysis of ion channel mutations. The SyncroPatch 384/768PE supports voltage- and current clamp recordings, temperature control, and minimal cell us- age. In addition to the use of stably transfected cell lines, more challeng- ing cell assays including stem cell-derived cells, transiently transfected cells or primary cells can be used successfully. The CardioExcyte 96, a device for label-free analysis of 2D/3D cells/ clusters in a 96 well plate, utilizes two different analysis technologies: Ex- tracellular field potential and impedance. It is a versatile tool for cardiac safety screening given its high resolution which allows the recording of beating iPSC-derived cardiomyocyte networks. The optical lid (Cardio- Excyte 96 SOL) uses LEDs for pacing cardiomyocytes with light (optoge- netics) to study beat rate-dependencies of compounds. Furthermore, long-term impedance measurements of cells over several days makes it an ideal tool for routine toxicity screening (e.g. hepatotox, cardiotox) and cell monitoring. Colin Coates, Product Manager, Andor Technology Chi-Li Chiu, Technical Support Specialist, Bitplane Exhibitor Presentation

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