Biophysical Society 62nd Annual Meeting | Program Guide

1078-Plat Education Travel Awardee ALTERNATIVE SRP RNA FOLDED STATES ACCESSIBLE CO-TRANSCRIPTION- ALLY CAN MODULATE SRP PROTEIN-TARGETING ACTIVITY.  Shingo Fukuda, Shannon Yan , Mingxuan Sun, Carlos J. Bustamante 1079-Plat 4:45 pm SIMULATIONS OF OPTICAL TWEEZERS EXPERIMENTS REVEAL DETAILS OF RNA STRUCTURE UNFOLDING. Wojciech K. Kasprzak , Taejin Kim, My-Tra Le, Feng Gao, Megan Y. L. Young, Xuefeng Yuan, Joonil Seog, Anne E. Simon, Bruce A. Shapiro 1080-Plat 5:00 pm RELATIONSHIP BETWEEN FOLDING AND CATALYSIS IN THE GLMS RIBO- ZYME RIBOSWITCH.  Andrew Savinov , Steven M. Block 1081-Plat 5:15 pm INVESTIGATING THE FUNCTION OF CONFORMATIONAL HETEROGENEITY IN TELOMERASE RNA USING MULTI-DIMENSIONAL CHEMICAL MAPPING AND SINGLE-MOLECULE SPECTROSCOPY.  Christina Palka , Rhiju Das, Yehuda Tzfati, Michael Stone 1082-Plat 5:30 pm A NANOIMAGING APPROACH FOR IDENTIFICATION OF THE SECONDARY STRUCTURAL DOMAINS IN LONG SSRNA MOLECULES.  Jamie L. Gilmore , Aiko Yoshida, Hideki Aizaki, Masahiro Nakano, Takaji Wakita, Shige Yoshimura, Kunio Takeyasu, Takeshi Noda 1083-Plat 5:45 pm CELLULAR IMAGING OF SMALL RNAS USING FLUORESCENT RNA-MANGO APTAMERS.  Adam Cawte , Sunny Jeng, Alexis Autour, Michaël Ryckelynck, Peter Unrau, David Rueda Platform 4:30 pm DETECTION OF METHYLATION ON DSDNA AT SINGLE-MOLECULE LEVEL USING SOLID-STATE NANOPORES.  Julian Bello, younghoon Kim, Shouvik Banerjee, Kirby Smithe, David Estrada, SuA Myong, Ann Nardulli, Eric Pop, Rashid Bashir, Jiwook Shim 1085-Plat 4:15 pm THE NANOPORE MASS SPECTROMETER. Mathilde Lepoitevin , William Maulbetsch, Benjamin Wiener, Derek Stein 1086-Plat 4:30 pm ACTIVE TRANSPORT BY A MEMBRANE EMBEDDED BIOMOTOR NANO- PORE.  Ke Sun, Yuejia Chen, Changjian Zhao, Xialin Zhang, Xiaojun Zeng, Xin Jiang, Jia Geng 1087-Plat 4:45 pm LABEL-FREE DETECTION OF SINGLE-MOLECULE MELTING KINETICS WITH LASER HEATED NANOPORE.  Hirohito Yamazaki , Rui Hu, Robert Henley, Justin Halman, Kirill Afonin, Dapeng Yu, Qing Zhao, Meni Wanunu 1088-Plat 5:00 pm NANOPARTICLE-GUIDED BIOMOLECULE DELIVERY FOR TRANSGENE EX- PRESSION AND GENE SILENCING IN MATURE PLANTS.  Gozde S. Demirer , Roger Chang, Huan Zhang, Linda Chio, Markita P. Landry 1089-Plat 5:15 pm GOLD NANOWIRE FABRICATION WITH SURFACE-ATTACHED LIPID NANO- TUBE TEMPLATES.  Kristina Jajcevic , Kaori Sugihara Micro- and Nanotechnology 4:00 pm–6:00 pm, Esplanade, Room 156 Co-Chairs Jiwook Shim, Rowan University Utku Sönmez, Carnegie Mellon University 1084-Plat 4:00 pm

1090-Plat 5:30 pm CHEMOTAXIS OF IMMUNE CELLS IN MICROFLUIDIC FLOW-FREE CON- CENTRATION GRADIENT GENERATOR.  Utku M. Sonmez , Philip R. LeDuc, Pawel Kalinski, Lance A. Davidson 1091-Plat 5:45 pm HIGH SPEED MOTORS DRIVEN BY A MOLECULAR TENSION GRADIENT.  Aaron T. Blanchard , Khalid Salaita Exhibitor Presentation Bruker Corporation 4:30 pm–6:00 pm, Exhibit Hall, Room 6 Harnessing the Power of Superresolution Single Molecule Localization Microscopy with the Vutura 352: Labeling and Imaging Strategies Single molecule localization microscopy (SMLM) has made a significant impact in the field of biology by enabling a 10-fold enhancement in reso- lution. A key factor in achieving this enhanced resolution is to optimally label and image the specimen. Numerous labeling strategies exist to tag structures in cells, bacteria, virus, tissue sections, C. elegans and Drosoph- ila , to make the best use of SMLM. Examples include DNA- and Oligo- Paint, antibody/nanobody labeling with organic dyes, Halo and SNAP-tag dyes, and photo-switchable fluorescent proteins. Choosing a sub-optimal labeling method for a given biological sample will result in loss of achiev- able resolution. Once a specimen has been optimally labeled and imaged, the acquired localization data can then be readily quantified via statistical analysis to test experimental hypotheses. Join this session to learn about labeling strategies and techniques used to get the best SML results. Speaker Manasa Gudheti, Sales Applications Scientist, Bruker Corporation Exhibitor Presentation Sutter Instrument 5:30 pm–7:00 pm, Exhibit Hall, Room 5 Scientists Empowering Scientists There have been many technological evolutions in Patch Clamp electro- physiology over the past 4.5 decades that Sutter Instrument has been collaborating with researchers.  During this period, Sutter has introduced many new product families, including pipette pullers, manipulators, light sources, wavelength switchers, specialized microscopes, and most recent- ly, fully integrated patch clamp amplifier systems. At this presentation, we will teach techniques, tips and tricks, and showcase features from three of our product families: pullers, manipulators, and patch clamp systems. Since Sutter Instrument’s inception in 1974, our pipette pullers have been used in a large number of research facilities all over the world.They are considered the unparalleled leader in performance and reliability. We will demonstrate how to make the unique micropipettes needed for your application, with a discussion on scoring and cutting, bending, polishing, and beveling. The IPA ® , Double IPA ®  and new dPatch ®  Ultra-fast, Low-noise Integrated Patch Clamp amplifiers, and SutterPatch ®  Software can be used for a variety of common experiments, including characterization of ionic cur- rent and recording synaptic events in tissue slices. We will demonstrate how the SutterPatch Software’s online measurements and sophisticated control of experimental workflow can be used to aid real-time decision- making and eventually simplify analysis. Sutter introduced Micromanipulators in 1985. From that time on, the company has continued to develop manipulators with stepper motor drive mechanisms and ergonomic controllers that are adaptable to many different experimental designs and platforms. We will introduce two new-

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