Biophysical Society 63rd Annual Meeting | Program Guide

Room 303: Sunday, March 3 9:30 AM – 11:00 AM Mizar Imaging Tilt – High-Resolution Light Sheet Imaging

2:30 PM – 4:00 PM Alvéole Bioengineering Relevant Cellular Microenvironments with Primo® In vivo, the cellular microenvironment has a crucial impact on the regula- tion of cell behavior and functions, such as cellular differentiation, prolif- eration and migration. One of the challenges confronting cell biologists is to mimic this microenvironment in vitro in order to more efficiently study living cells and model diseases. To this end, we present the PRIMO device developed by ALVEOLE. This contactless and maskless UV projec- tion system based on the LIMAP technology(1) allows to control the bio- chemical and mechanical properties of in vitro microenvironments. We will first show that PRIMO is a suitable tool to print biomolecules on sub- strates (including glass, plastic, soft/stiff substrates, textured surfaces, etc.) with an exquisite control over protein densities (micropatterning). Then, we will also present how the projected UV light can be used in order to structure photosensitive resists (such as SU8) and create molds onto which elastomeric solutions can be polymerized (microfabrication). Finally, one of our users will share his research conducted with PRIMO. He used this technology in order to structure and functionalize hydrogels (microstructuration combined with micropatterning) paving the way for 3D cell culture onto controlled, reproducible soft substrates(2). Visit www.alveolelab.com for more information. Speakers Aurélien Pasturel, University of Bordeaux, CNRS, Alvéole Pierre-Olivier Strale, Senior Scientist, Alvéole 4:30 PM – 6:00 PM Molecular Devices Supercharge Your Patch-Clamp Data Acquisition and Analy- sis with the New Axon pCLAMP 11 Software The patch-clamp technique remains the best method for examining ion channel physiology and membrane biophysics. Axon Instruments and pCLAMP software continue to push the envelope with new innovations with best-in-class systems and software. In this user meeting we learn about new features of pCLAMP 11 software and methods to optimize your workflow and simplify experiments. Speaker Jeffrey Tang, Senior Global Axon Electrophysiological Application Scientist, Molecular Devices

Mizar Imaging is proud to introduce the Tilt, the first high-resolution light sheet imaging system that is a simple add-on to most inverted micro- scopes. When installed on your microscope, the Tilt does not interfere with any existing modalities so you can easily add the Tilt to an inverted microscope, including a TIRF or Spinning Disc confocal microscope sys- tem, to add the ability to do long term live cell imaging with the lowest possible photobleaching and phototoxicity. The Tilt is well-suited to image both larger organisms, such as C. elegans, Drosophila, Danio rerio and other similar model organisms as well as imaging high-resolution intracellular dynamics inside single cells. This remarkable diversity is realized because the Tilt can work with any objec- tive on your microscope – from 20x through 150x. There is no limit to what you can do with the Tilt. The key benefit of light sheet imaging is significantly reducing the pho- tobleaching and phototoxicity of your sample. The Tilt is no exception. When imaging with the Tilt, cells can be kept alive for hours and even days. This is aided by an optional incubation chamber for the Tilt, which allows for precise control of temperature (heating and cooling available), CO2 and humidity. The Tilt light-sheet imaging system is the ideal solution for long-term live-cell imaging of a wide array of samples with the added benefit of being a simple, low cost add-on to an existing inverted microscope. Speaker Chris Baumann, Sales and Product Manager, Mizar Imaging

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