Biophysical Society 63rd Annual Meeting | Program Guide

1:30 PM – 3:00 PM Carl Zeiss Microscopy LLC ZEISS Elyra 7 with Lattice SIM, a New Platform for Fast and Gentle 3D Superresolution Microscopy Life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of the sample. Whether you are working with tissue, bacteria, organoids, neurons, liv- ing or fixed cells, ZEISS Elyra 7 takes your images beyond the diffraction limit of conventional microscopy to superresolution. Examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors. Lattice SIM enables fast imaging of 3D volumes with resolution down to 120 nm laterally and 300 nm axially. Due to higher light efficiency, the new Lattice SIM technology provides gentle superresolution imag- ing of living specimens at up to 255 frames per second. Using less light to illuminate the specimen means imaging longer with less bleaching of the sample. The novel Lattice SIM technology allows you to uncover new mechanistic details and quantify the finest subcellular structures in large fields of view. ZEISS Elyra 7 can be expanded with single molecule localization micros- copy (SMLM) for techniques such as PALM, dSTORM and PAINT. ZEISS Elyra 7’s SMLM module delivers molecular resolution in large 3D volumes and powerful post-processing algorithms for quantification. Choose freely among labels when imaging with resolutions down to 20 nm laterally and 50 nm axially. Count molecules and come to under- stand, molecule-by-molecule, how individual proteins are arranged within a structural context. ZEISS Elyra 7 is a flexible research grade live cell microscope from ZEISS. The new Apotome mode allows fast optical sectioning of 3D samples and total internal reflection microscopy provides live imaging capability for membrane and single molecule studies. Join this workshop and learn how the newest member of the ZEISS imaging portfolio, ZEISS Elyra 7, can help your imaging experiments in completely new ways. Speaker Renée Dalrymple, Sales Development Manager, Carl Zeiss Microscopy LLC

11:30 AM – 1:00 PM Leica Microsystems Leica SP8 FALCON: A New Way to Generate Fluorescence Lifetime Images at Confocal Speed Functional imaging is a rapidly growing field, because understanding the function and interaction of molecules is the key to revealing the under- lying biology. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artifacts and sensitive to molecular environment, but previous FLIM solutions were slow and difficult to implement, particularly for complex imaging workflows. Therefore, FLIM imaging has so far been limited to specialized laboratories and classical TCSPC has been unable to deliver the speeds needed to address most of the biological processes. We present SP8 FALCON, the fast, intuitive and totally integrated all- Leica FLIM solution. SP8 FALCON delivers video-rate FLIM with pixel-by- pixel quantification, thanks to a unique combination of fast electronics, sensitive spectral hybrid detectors (Leica HyDs), and a novel concept for measuring time. Photon arrival times can now be recorded at count rates typical for standard confocal imaging. The system has ultra-short dead time, and powerful built-in algorithms take care of the data acquisition and analysis, while keeping accuracy and excellent data quality. This talk explains the technical implementations enabling this new level of perfor- mance and explains the new way to generate FLIM images. SP8 FALCON with STED enables STED-FCS at high count rate and separa- tion of multiple fluorophores spectrally overlapping with nanoscopic resolution. SP8 DIVE (Leica multiphoton system) with spectrally tunable non-des- canned detector (Leica 4Tune detector) combined with FALCON allows metabolic imaging, species separation and in vivo FLIM imaging. The deep integration of SP8 FALCON into the Leica SP8 platform provides easy access to complex FLIM experiments, enabling fast FLIM-FRET, 3D- and 4D-imaging modes, high-content screening, and autofluorescence component separation.

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