Biophysical Society 63rd Annual Meeting | Program Guide

3:30 PM – 5:00 PM Wyatt Technology Corporation From Proteins to Exosomes: Tools for Essential Biophysical QC, Characterization, and Isolation In this seminar we will present solutions for some of the key biophysical characterization challenges encountered in the course of biophysical research. The tools to overcome these challenges are based on: • multi-angle light scattering (MALS) for determining absolute molar mass and size of macromolecules and nanoparticles from small peptides to vesicles; • dynamic light scattering (DLS) for determining the hydrodynamic radii of particles from 0.2 to 5000 nm; • asymmetric-flow field-flow fractionation (AF4) for separation and characterization of particle distributions from 1 nm to 10 µm • composition-gradient MALS (CG-MALS) for label-free analysis of biomolecular interactions to determine binding affinity and abso- lute stoichiometry in solution The combination of these measurement techniques with each other and with other methods of automated sample preparation and delivery cre- ates a powerful toolkit that is useful across many fields of experimental bioscience. The presentation will include applications to: • quality control of proteins and other biomacromolecules to ensure reliable, repeatable studies of structure and interactions • rapid optimization of crystallization conditions • analysis of oligomeric state, protein-protein and protein-nucleic acid complexes • understanding self-assembly, aggregation and fibril formation • characterization of vesicle size and content, and high-resolution size-based isolation of exosomes and exomeres. In addition to describing the principles and instrumentation of SEC- MALS, AF4-MALS, CG-MALS and DLS, we will perform a live demo of pro- tein and buffer characterization by automated DLS in microwell plates. Speaker Eric Seymour, Senior Application Scientist, Wyatt Technology Corporation

5:30 PM – 7:00 PM ELEMENTS SRL Portable and Cost-Effective Low-Noise Amplifiers for Elec- trophysiology and Nanopore Applications Ultra-portable and cost-effective amplifier technology is now a reality accessible to any electrophysiology research lab, thanks to Elements microelectronic-based design of custommicrochip (ASIC) using standard and low-cost CMOS processes. Elements provides an integrative solid-state solution to measure cur- rents in the picoampere (10-12 pA) range, with bandwidths up to hun- dreds of kHz, featuring very low noise recordings, signal digitalization thanks to the internal Analog-to-Digital converter, signal generator, digital data elaboration,and USB powered, all in a tiny form factor (i.e. 42x18x78 mm) or about the size of a point-and-shoot digital camera! In this presentation, we will be featuring our latest electrophysiology product, the world’s smallest integrated patch clamp amplifier, as well as a portable nanopore kit for protein detection using disposable glass nanopore chips. During the event will be presented these two use cases: 1. ePatch amplifier was used to record the current of HCN chan- nels transiently expressed in HEK293T cells, with the aim to test the effect of Lamotrigine, a widely used anticonvulsant drug, on the biophysical proprieties of the current. Data courtesy of Dr. A. Moroni - University of Milan - Italy and Dr. Bina Santoro - Columbia University - New York – USA 2. Portable Nanopore Reader: example of DNA fragment translo- cations through glass nanopore chips. Data courtesy of Dr. D. Niedzwiecki, Goeppert– USA Attend this presentation to learn about: • The advantages of using a versatile and compact nano-current amplifier technology, • Portable nanopore solution for protein detection using disposable nanopore chips, • How the world smallest and cheapest patch clamp amplifier is radi- cally changing voltage-clamp measurements! Complimentary Italian hors d’oeuvres and drinks will be served! Seating is limited. Be the first to RSVP by emailing info@elements-ic.com to receive a copy of the presentation and be entered in a raffle to receive a free 30-day trial of the ePatch or nanopore Kit amplifier! Speakers Federico Thei, CEO, ELEMENTS SRL Filippo Cona, Software Engineer, ELEMENTS SRL Alessandro Porro, Application Scientist, ELEMENTS SRL Serge Kaddoura, NanoscaleLABS

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