Biophysical Society 63rd Annual Meeting | Program Guide

1:30 PM – 3:00 PM Bruker Corporation

3:30 PM – 5:00 PM NanoSurface Biomedical Biomimetic Cell Culture Platforms for Enhancing Cell Biol- ogy Studies Cells use structural and mechanical cues from the extracellular matrix (ECM) to regulate a broad spectrum of processes such as cell signaling, electrophysiology, differentiation, division, and even life and death. Over the past few decades, the literature has demonstrated that many cell types cultured in conventional flat, rigid, and static culture condi- tions lack both structural and functional phenotypes seen in the body, and that the lack of extracellular cues contributes significantly to the disconnect between in vitro experimental results and in vivo observa- tion. We will demonstrate that ECM-inspired substrate nanotopography drastically improves the structural and functional development of a vari- ety of cell types. Specifically, we show how NanoSurface Cultureware and the NanoSurface Cytostretcher can be utilized to study the effects of cell-nanotopography interactions on adhesion, signaling, polarity, migra- tion, physiology, and differentiation across many cell types and model systems including cancer biology, human epithelia, and cardiovascular function. Further, we will describe how the differentiation of induced pluripotent stem cells can be accelerated and enhanced by providing a more biomimetic culture environment. We will also illustrate how the combination of nanotopography and mechanical stretch can enhance the in vitro phenotypes of cells in culture. Speaker Nicholas Geisse, Chief Science Officer, NanoSurface Biomedical

Investigating Dynamic Biological Processes with High- Speed, High-Resolution Correlative AFM-Light Microscopy The ability of atomic force microscopy (AFM) to obtain three-dimen- sional topography images of biological molecules and complexes with nanometer resolution and under near-physiological conditions remains unmatched by other imaging techniques. However, the typically longer image acquisition times required to obtain a single high-resolution image (~minutes) has limited the advancement of AFM for investigating dynamic biological processes. While recent years have shown significant progress in the development of high-speed AFM (HS-AFM), the ability to scan faster has typically been achieved at the cost of decreased scanner range and restricted sample size. As such, these HS-AFM systems have mainly been focused on studying single molecule dynamics and have been very limited in their ability to conduct live cell imaging. The novel NanoWizard® ULTRA Speed A AFM not only enables high- speed studies of time-resolved dynamics associated with cellular pro- cesses, it’s latest scanner technologies and compact design also allow full integration of AFM into advanced commercially available light microscopy techniques. Thus, fast AFM imaging of several frames per second can be seamlessly combined with methods such as epifluores- cence, confocal, TIRF, STED microscopy, and many more. Please join us for this informative seminar where we will present how the latest advances in the ULTRA Speed A AFM are being applied to study a wide-range of biological samples, from individual biomolecules to mam- malian cells and tissues. We will also describe how this unique system is enabling new research opportunities with high-speed, highresolution correlative AFM-light microscopy. Speaker Andrea Slade, BioAFM Product Manager, JPK BioAFM Center, Bruker Nano Surfaces

168