Biophysical Society 63rd Annual Meeting | Program Guide

Exhibitor Presentation Leica Microsystems 11:30 am - 1:00 pm, Room 303 LEICA SP8 FALCON: A NEWWAY TO GENERATE FLUORESCENCE LIFETIME IMAGES AT CONFOCAL SPEED Functional imaging is a rapidly growing field, because understanding the function and interaction of molecules is the key to revealing the underlying biology. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artifacts and sensitive to molecular environment, but previous FLIM solutions were slow and difficult to implement, particularly for complex imaging workflows. Therefore, FLIM imaging has so far been limited to specialized laboratories and classical TCSPC has been unable to deliver the speeds needed to address most of the biological processes. We present SP8 FALCON, the fast, intuitive and totally integrated all-Leica FLIM solution. SP8 FALCON delivers video-rate FLIMwith pixel-by-pixel quantification, thanks to a unique combination of fast electronics, sensitive spectral hybrid detectors (Leica HyDs), and a novel concept for measuring time. Photon arrival times can now be recorded at count rates typical for standard confocal imaging. The system has ultra-short dead time, and powerful built-in algorithms take care of the data acquisition and analysis, while keeping accuracy and excellent data quality. This talk explains the technical implementations enabling this new level of performance and explains the new way to generate FLIM images. SP8 FALCON with STED enables STED-FCS at high count rate and separation of multiple fluorophores spectrally overlapping with nano- scopic resolution. SP8 DIVE (Leica multiphoton system) with spectrally tunable non-descanned detector (Leica 4Tune detector) combined with FALCON allows metabolic imaging, species separation and in vivo FLIM imaging. The deep integration of SP8 FALCON into the Leica SP8 platform provides easy access to complex FLIM experiments, enabling fast FLIM-FRET, 3D- and 4D-imaging modes, high-content screening, and autofluorescence component separation. Career Development Center Workshop Demystifying the Academic Job Search I: Understanding the Search Process from the Perspective of Search Committees and Decoding Job Announcements 12:00 pm - 1:00 pm, Exhibit Hall A What goes on inside search committees; the “black box” of the academic job search process? How are they constituted, what are their processes, and what do they look for when assessing applicants? Answers to these and other questions presented by Andrew Green, PhD a veteran of the academic job search and numerous search c ommittees. Public Affairs Committee Meeting 12:15 pm - 2:15 pm, Room 333 The World Outside the Lab Many Ways to Use Your PhD Skills 1:00 pm - 2:30 pm, Room 318/319/320 Have you ever wondered how you can apply the skills learned while working on your PhD in a career away from the bench? This panel will explore multiple career options that exist in government, industry, and

165-Plat 11:30 am NANOPORE DETECTION OF SURPRISING MOLARITY DEPENDENCE OF DNA KNOT COMPLEXITY.  Rajesh K. Sharma , Liang Dai, Ishita Agrawal, Patrick S. Doyle, Slaven Garaj 166-Plat 11:45 am NANOCARRIERS FOR MAGNETICALLY ACTUATED TARGETED DRUG DE- LIVERY.  Vrinda Sant , Liping Wang, Grace Jang, Deependra Ban, Jay Seth, Sami Kazmi, Nirav R. Patel, Qingqing Yang, Joon Lee, Woraphong Janeta- nakit, Shanshan Wang, Brian Head, Gennadi Glinsky, Ratnesh Lal 10 167-Plat 12:00 pm QUANTIFYING THE INFLUENCE OF NANOPARTICLE POLYDISPERSITY ON CELLULAR DELIVERED DOSE.  Stuart Johnston , Matthew Faria, Edmund 12:15 pm REVEALING THE DYNAMICS OF SINGLE-MOLECULE REACTIONS IN A SINGLE-MOLECULE NANOREACTOR.  Kaipei Qiu , Bo Yuan, Yi-Tao Long 169-Plat 12:30 pm DEVELOPMENT OF A NEW ANTIMICROBIAL PHOTOSENSITIZER FROM BROMINATED CARBON DOTS: METAL-ENHANCED PHOSPHORESCENCE AND SINGLET OXYGEN GENERATION.  Rachael Knoblauch , Christopher D. Geddes Exploring Careers in Biophysics Day 11:15 am - 3:00 pm, Room 321/322/323 This free day for Baltimore-area high school and college students at the BPS 63rd Annual Meeting kicks off with an Undergraduate Student Pizza “Breakfast” which will include a panel discussion on academic and career paths in biophysics. Come prepared to find out about the course of study that aspiring biophysicists undertake, what it means to be a biophysicist, and how biophysicists make important discoveries. Students will also receive information and advice on how to get the most out of attending the Annual Meeting. Attendees may attend any of the meeting’s open sessions and activities for the full day, including the Education & Career Opportunities Fair where they can meet with representatives of, and learn about, opportunities from around the world. In addition, there will be some fun, interactive demos for students to learn about ground- breaking techniques in the field. Pre-registration was required. No onsite registration. Undergraduate Student Pizza “Breakfast” 11:30 am - 1:00 pm, Room 321/322/323 This “breakfast” for undergraduate students offers a valuable networking and social opportunity to meet other students, Biophysical Society Committee members, and scientists at all career levels to discuss academic goals and questions, and to develop a biophysics career path. The Breakfast will include a panel discussion on academic and career paths in biophysics, with opportunities for questions and answers from the audience - come prepared to find out about the course of study that aspiring biophysicists undertake, what it means to be a biophysicist, and how biophysicists make important discoveries. Space for this session is limited to the first 100 attendees. Speakers Elih Velázquez, Naval Medical Research Center Crampin 168-Plat

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Logan Kaler, University of Maryland Ashley Simpson, Bay Path University

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