Biophysical Society 63rd Annual Meeting | Program Guide

Exhibitor Presentation Wyatt Technology Corporation 3:30 pm - 5:00 pm, Room 303 FROM PROTEINS TO EXOSOMES: TOOLS FOR ESSENTIAL BIOPHYSICAL QC, CHARACTERIZATION, AND ISOLATION In this seminar we will present solutions for some of the key biophysical characterization challengesencountered in the course of bio- physical research. The tools to overcome these challenges are based on: • multi-angle light scattering (MALS) for determining absolute molar mass and size of macromolecules and nanoparticles from small peptides to vesicles; • dynamic light scattering (DLS) for determining the hydrodynamic radii of particles from 0.2 to 5000 nm; • asymmetric-flow field-flow fractionation (AF4) for separation and characterization of particle distributions from 1 nm to 10 µm • composition-gradient MALS (CG-MALS) for label-free analysis of biomolecular interactions to determine binding affinity and absolute stoichiometry in solution The combination of these measurement techniques with each other and with other methods of automated sample preparation and delivery creates a powerful toolkit that is useful across many fields of experimen- tal bioscience. The presentation will include applications to: • quality control of proteins and other biomacromolecules to ensure reliable, repeatable studies of structure and interactions • rapid optimization of crystallization conditions • analysis of oligomeric state, protein-protein and protein-nucleic acid complexes • understanding self-assembly, aggregation and fibril formation • characterization of vesicle size and content, and high-resolution size-based isolation of exosomes and exomeres. In addition to describing the principles and instrumentation of SEC-MALS, AF4-MALS, CG-MALS and DLS, we will perform a live demo of protein and buffer characterization by automated DLS in microwell plates. Speaker Eric Seymour, Senior Application Scientist, Wyatt Technology Corporation Career Development Center Workshop Nailing the Job Talk, or Erudition Ain’t Enough 4:00 pm - 5:00 pm, Exhibit Hall A Congratulations! You’ve made it to the finals and are suddenly facing the most important presentation of your life. Answers to your ques- tions about how to structure your presentation, how much detail to include, what they are really looking for, etc. Symposium Integrative Modeling from Macromolecules to Cell 4:00 pm - 6:00 pm, Ballroom I Chair Zaida Ann Luthey-Schulten, University of Illinois at Urbana-Champaign 170-Symp 4:00 pm MAPPING THE SPATIAL ORGANIZATION OF GENOMES THROUGH DATA INTEGRATION.  Frank Alber , Polles Guido, Hua Nan, Yildirim Asli, Zhan Yuxiang 171-Symp 4:30 pm MULTISCALE MODELING OF BIOMOLECULAR PROCESSES BY COMBINING EXPERIMENT AND SIMULATION.  Cecilia Clementi 172-Symp 5:00 pm DEALING WITH DYNAMICS AND DISORDER BY COMBINING SIMULATION AND EXPERIMENT.  Gerhard Hummer

173-Symp 5:30 pm TOWARDS SIMULATING BACTERIAL AND EUKARYOTIC CELLS: INTEGRA- TION OF EXPERIMENT AND THEORY.  Zaida Ann Luthey-Schulten Symposium Cytoskeleton 4:00 pm - 6:00 pm, Ballroom II Chair Sabine Petry, Princeton University 174-Symp 4:00 pm PHASE SEPARATION OF TPX2 ENHANCES AND SPATIALLY BIASES MICRO- TUBULE NUCLEATION.  Sabine Petry 175-Symp 4:30 pm REGULATION OF BIDIRECTIONAL MOTILITY OF KINESIN-5 MOTORS.  Leah Gheber 176-Symp 5:00 pm REGULATION OF MYOSIN MOTORS - FROM SINGLE MOLECULES TO FUNCTIONAL ENSEMBLES.  Claudia Veigel 177-Symp 5:30 pm THE MYOSIN MESA AND HYPERTROPHIC CARDIOMYOPATHY: MUTATIONS TO MECHANISMS TO THERAPIES.  James Spudich Platform Co-Chairs Sonja Minniberger, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Germany Mufeng Li, NINDS, NIH 178-Plat 4:00 pm HIGH THROUGHPUT VALIDATION OF NON CANONICAL AMINO ACID INCORPORATION INTO ACID SENSING ION CHANNEL 1A.  Nina Braun , Søren Friis, Weihua Tian, Eric P. Bennett, Jacob Andersen, Stephan A. Pless 179-Plat 4:15 pm TRAVEL AWARDEE MEASURING DYNAMICS OF THE ACID-SENSING ION CHANNEL N-TER- MINUS USING TRANSITION METAL ION FRET. Megan Cullinan , Prafulla Aryal, John Bankston 180-Plat 4:30 pm A MECHANISM FOR DESENSITIZATION OF ALL THREE FUNCTIONAL MAMMALIAN ACID SENSING ION CHANNELS.  Yangyu Wu, Zhuyuan Chen, Cecilia Canessa 181-Plat 4:45 pm A CRITICAL MOBILE DIVALENT CATION SITE IN THE ATP-BINDING POCK- ET OF P2X3 RECEPTORS THAT CONTROLS CHANNEL GATING. Mufeng Li , Yao Wang, Shai D. Silberberg, Motoyuki Hattori, Kenton Swartz 182-Plat 5:00 pm STRUCTURAL STUDIES OF MUTANTS OF THE NAK CHANNEL.  Sonja Minniberger , Saeid Abdolvand, Han Sun, Andrew J. Plested 183-Plat 5:15 pm ROLE OF NMDAR-BK COMPLEXES IN THE INTEGRATION OF SYNAPTIC INPUTS OF BARREL CORTEX PYRAMIDAL NEURONS.  Ricardo Gómez , Laura E. Maglio, Alberto J. Gonzalez-Hernandez, Belinda Rivero-Perez, Teresa Giraldez 184-Plat 5:30 pm PROBING STRUCTURAL STATES OF A PENTAMERIC LIGAND-GATED ION CHANNEL WITH SMALL ANGLE NEUTRON SCATTERING. Marie Lycksell , Nicolai T. Johansen, Rebecca J. Howard, Lise Arleth, Erik Lindahl Ligand-gated Channels 4:00 pm - 6:00 pm, Ballroom III

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