Biophysical Society 63rd Annual Meeting | Program Guide

Platform Protein Assemblies/Enzyme Function, Cofactors and Post-translational Modifications I 8:15 am - 10:15 am, Room 314/315 Co-Chairs Peter Schuck, NIBIB NIH Charlotte Lorenz, Forschungszentrum Jülich, Germany 781-Plat 8:15 am MEASURING MACROMOLECULAR SIZE-DISTRIBUTIONS AND INTERAC- TIONS AT HIGH CONCENTRATIONS BY SEDIMENTATION VELOCITY.  Sumit K. Chaturvedi, Jia Ma, Patrick H. Brown, Huaying Zhao, Peter Schuck 782-Plat 8:30 am PHYSIOLOGICALLY-RELEVANT CROWDING EFFECTS ON THE SH3-SON OF SEVENLESS INTERACTION.  Samantha S. Stadmiller , Jhoan Sebastian Aguilar, Gary J. Pielak 783-Plat 8:45 am A CONSERVED ASPARAGINE IN A UBIQUITIN CONJUGATING ENZYME PROMOTES A REACTIVE SUBSTRATE GEOMETRY.  Isaiah Sumner , Walker M. Jones, Aaron G. Davis, R. Hunter Wilson, Katherine L. Elliott 784-Plat 9:00 am DIRECT OBSERVATION OF PROTEIN TRANSLOCATION BY THE 26S PROTEASOME.  Erik Jonsson , Jared Bard, Erika M. López-Alfonzo, Ellen Goodall, Ken Dong, Andreas Martin 785-Plat 9:15 am CHARACTERIZATION OF THE ASSEMBLY AND DISASSEMBLY OF CAPSID PROTEINS DERIVED FROM HEPATITIS B VIRUS. Maelenn Chevreuil , Sonia Fieulaine, Laetitia Poncet, Karen Perronet, Thomas Zinn, Eric Jac- quet, Naima Nhiri, Stephane Bressanelli, Guillaume Tresset 786-Plat 9:30 am TRAVEL AWARDEE ASSEMBLY MECHANISM OF FARNESYLATED HGBP1 STUDIED BY TIME- RESOLVED SAXS AND ELECTRON MICROSCOPY.  Charlotte Lorenz , Andreas M. Stadler 787-Plat 9:45 am PROTEIN SELF-ASSEMBLY DRIVES SURFACE LAYER BIOGENESIS AND MAINTENANCE IN C. CRESCENTUS .  Jonathan Herrmann , Colin Comerci, Joshua Yoon, Fatemeh Jabbarpour, Lucy Shapiro, Soichi Wakatsuki, Wil- liam E. Moerner 788-Plat 10:00 am QUENCHABLE PROBES FOR IMAGING OXIDATIVE STRESS IN VIVO.  Oshini Ekanayake , Sam Scinto, Joseph Fox, Sharon Rozovsky Platform Biomolecular Methods In and Out of Cells 8:15 am - 10:15 am, Room 316/317 Co-Chairs Ryan Russell, University of Delaware Jill Trewhella, The University of Sydney, Australia 789-Plat 8:15 am ATOMIC STRUCTURE OF NEARLY INDESTRUCTIBLE PILI FROM A HYPER- THERMOPHILIC ACIDOPHILE.  Fengbin Wang , Virginija Cvirkaite-Krupo- vic, Joe S. Wall, David Prangishvili, Mart Krupovic, Edward H. Egelman 790-Plat 8:30 am CHARACTERIZING FUNCTIONAL STATES OF A MODEL LIGAND-GATED ION CHANNEL BY CRYO-ELECTRON MICROSCOPY.  Urska Rovsnik , Re- becca Howard, Bjorn Forsberg, Marta Carroni, Erik Lindahl

791-Plat TRAVEL AWARDEE CRYO-EM STRUCTURES REVEAL MECHANISMS OF ACTIVATION AND INACTIVATION IN BESTROPHIN CHANNELS.  Alexandria N. Miller , George Vaisey, Stephen B. Long 792-Plat 9:00 am OUTCOMES OF THE CRYO-EMMAP AND MODEL CHALLENGES.  Catherine L. Lawson , Andriy Kryshtafovych, Grigore Pintilie, Helen M. Berman, Wah Chiu 793-Plat 9:15 am A COMPLETE ATOMIC MODEL FOR LETHOCERUS FLIGHT MUSCLE MYOSIN FILAMENT.  Hamidreza Rahmani , Nadia Daneshparvar, Zhongjun Hu, Dianne Taylor, Robert J. Edwards, Kenneth A. Taylor 794-Plat 9:30 am COMPUTATIONAL ASSESSMENT OF DISTANCE RESTRAINT REQUIRE- MENTS FOR ACCURATE PROTEIN STRUCTURE DETERMINATION BY MAS NMR.  Ryan W. Russell , Matthew Fritz, Jodi Kraus, Caitlin M. Quinn, Angela M. Gronenborn, Tatyana Polenova 795-Plat 9:45 am INTRACELLULAR METAL SPECIATION IN STREPTOCOCCUS SANGUINIS IS PIVOTAL FOR REDOX MAINTENANCE.  Cody Murgas , Ashley K. Forney, Shannon Baker, Seon-Sook An, Todd O. Kitten, Heather R. Lucas 796-Plat 10:00 am RELIABLE BIOMOLECULAR STRUCTURAL MODELLING WITH SMALL-AN- GLE SCATTERING.  Jill Trewhella CPOW Committee Meeting 8:30 am - 10:30 am, Room 333 Exhibitor Presentation Bruker Corporation 9:30 am - 11:00 am, Room 303 ADVANCES IN DYE DEVELOPMENT AND MICROSCOPY FOR LIVE CELL SUPER RESOLUTION MICROSCOPY WITH THE VUTARA 352 Expanding the frontier of super-resolution imaging requires advances in both microscopy hardware and fluorescent labels. Here we describe a cooperative effort to improve both technological fronts with the ultimate goal of live-cell super-resolution microscopy. Bruker’s Vutara 352 super- resolution microscope has been designed for live-cell super-resolution microscopy with both high spatial and temporal resolution capabilities. The patented biplane module allows simultaneous two-color imaging in 3D while the sCMOS detector enables fast imaging of biological phenom- ena. Although this microscope system is capable of live-cell super-resolu- tion imaging, it has been stymied by limitations in the current generation of live-cell-compatible fluorophores. Extant live-cell probes are either fluorescent proteins with low photon counts—and therefore low localiza- tion precision—or organic dyes, which require high laser power resulting in phototoxicity in living samples. To remedy this problem, we developed spontaneously blinking (SB) versions of the Janelia Fluor and Alexa Fluor dyes, which blink under physiological conditions at low laser power while still providing high photon counts. In particular, the spontaneously blink- ing Janelia Fluor 549 (SB-JF549) and red-shifted SB-JF646 are cell-perme- able and are easily conjugated to HaloTag or SNAP-tag ligands, making them ready to use in live cell multi-color superresolution experiments. The SB dyes, in combination with the Vutara 352, provide a powerful methodology for simultaneous imaging, localization and visualization of live-cell single-molecule localization data, while offering numerous statis- tical tools to quantify the data into publishable results. Speaker Robert Hobson, Applications Scientist, Bruker Corporation 8:45 am

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