Biophysical Society 63rd Annual Meeting | Program Guide

Platform Macromolecular Interactions and Effects on Membranes 4:00 pm - 6:00 pm, Room 316/317 Co-Chairs Amanda Ward, University of Virginia Syma Khalid, University of Southampton, United Kingdom 890-Plat 4:00 pm THE STRUCTURAL BASIS OF A MEMBRANE-BOUND ESCRT-III HELICAL AS- SEMBLY.  Henry C. Nguyen , Nathaniel Talledge, John McCullough, Wesley I. Sundquist, Adam Frost 891-Plat 4:15 pm CATCHING HIV IN THE ACT OF FUSION: INSIGHT FROM CRYO-ET IN- TERMEDIATES OF HIV MEMBRANE FUSION.  Amanda E. Ward , Kelly A. Dryden, Lukas K. Tamm, Barbie K. Ganser-Pornillos 892-Plat 4:30 pm PROBING MEMBRANE FUSION INTERMEDIATES USING BILAYER COATED NANOPARTICLES.  Ana Villamil , Peter Kasson 893-Plat 4:45 pm BACTERIAL OUTER MEMBRANE VESICLE INTERACTION WITH PLASMA MEMBRANES: INSIGHTS FROMMOLECULAR SIMULATIONS.  Damien F. Jefferies, Anna L. Duncan, Syma Khalid 894-Plat 5:00 pm LIPID SPONGE-PHASE NANOPARTICLES AS ENZYME CARRIERS - STRUC- TURE AND INTERMOLECULAR INTERACTION CONTROLLING THE ENZYME INCLUSION. Maria Valldeperas, Najet Mahmoudi, Susana C. M. Teixeira, Martynas Talaikis, Ieva Matulaitienė, Gediminas Niaura, Justas Barauskas, Tommy Nylander 895-Plat 5:15 pm PREPARING ENDOSOME-DERIVED SUPPORTED MEMBRANES TO STUDY EBOLA VIRUS GP-MEDIATED MEMBRANE BINDING AND FUSION.  Laura Odongo 896-Plat 5:30 pm DETECTING AND CONTROLLING DYE AND ILLUMINATION EFFECTS IN SINGLE-VIRUS FUSION EXPERIMENTS.  Robert J. Rawle, Steven G. Boxer, Peter M. Kasson 897-Plat 5:45 pm THE SARS-COV FUSION PEPTIDE FORMS AN EXTENDED BIPARTITE FU- SION PLATFORM THAT PERTURBS MEMBRANE ORDER IN A CALCIUM- DEPENDENT MANNER.  Alex L. Lai , Jean K. Miller, Susan Daniel, Gary R. Whittaker, Jack H. Freed Exhibitor Presentation Molecular Devices 4:30 pm - 6:00 pm, Room 301 SUPERCHARGE YOUR PATCH-CLAMP DATA ACQUISITION AND ANALYSIS WITH THE NEW AXON pCLAMP 11 SOFTWARE The patch-clamp technique remains the best method for examining ion channel physiology and membrane biophysics. Axon Instruments and pCLAMP software continue to push the envelope with new innovations with best-in-class systems and software. In this user meeting we learn about new features of pCLAMP 11 software and methods to optimize your workflow and simplify experiments. Speaker Jeffrey Tang, Senior Global Axon Electrophysiological Application Scien- tist, Molecular Devices

Exhibitor Presentation LUMICKS 5:30 pm - 7:00 pm, Room 303 A VERSATILE PLATFORM FOR HIGH-RESOLUTION SINGLE-MOLECULE RESEARCH: EXPANDING CAPABILITIES AND EXPLORING NEW POSSIBILITIES Proteins interact with nucleic acids and the cytoskeleton to perform biological processes that are key to cell metabolism and life. The direct observation of such interactions in real time and at the singlemolecule enable scientists to make new discoveries and to test current biologi- cal models. Singlemolecule studies of cytoskeleton filaments and their interaction to associated proteins are often developed in surface-based assays where the glass surface is used as a substrate to rigidly anchor the biological molecules of interest. To capture the dynamics of the system and its interactions, the samples are typically labeled with fluorescent dyes and are imaged with fluorescence methods. However, despite the versatility of fluorescent methods, label-free imaging methods are desirable to better mimic the native biological conditions and to reduce photo-damage due to fluorescence excitation during long experiments. Here, we present our recent developments to further enable discover- ies in the field of biology and biophysics with a special focus in surface- based assays. We present a novel instrument arrangement that includes optical tweezers in combination with Interference Reflection Microscopy (IRM) and Total Internal Reflection Fluorescence (TIRF) Microscopy. IRM is a recently introduced imaging method that allows visualization of biological structures in 3D without the need for fluorescence labeling and with sensitivity exceeding that of Differential Interference Contrast (DIC) microscopy. In addition, we show the latest applications of these technologies and how they enhance our understanding of several fields of biology, includ- ing molecular motors and cytoskeleton filaments, DNA/RNA-protein interactions, protein folding/unfolding, cell membranes, and genome structure and organization. These applications show that the techno- logical advances in hybrid single-molecule methods for imaging and manipulation can be turned into easy-to-use and stable instruments with the ability to open up new venues in many research areas. Speakers Andrea Candelli, Application Scientist, LUMICKS Dinner Meet-Ups 6:00 pm - 6:30 pm, Society Booth/Charles Street Lobby Interested in making new acquaintances and experiencing the cuisine of Baltimore? Meet at the Society Booth each evening, Sunday through Tuesday, at 6:00 pm where a BPS member will coordinate dinner at a local restaurant. Awards and 2019 Biophysical Society Lecture 8:00 pm - 9:00 pm, Ballrooms I-IV Reception and Dance 9:30 pm - 12:00 am, Hilton, Key/Ballroom Sara Tafoya, Application Scientist, LUMICKS Trey Simpson, Application Scientist, LUMICKS

M O N D A Y

Reception and Quiet Room 9:30 pm - 12:00 am, Hilton, Peale A/C

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