Biophysical Society 65th Annual Meeting Program Guide

Exhibitor Presentation Nikon Instruments Inc 3:30 pm - 4:00 pm Using the Nikon Ti2 Inverted Platform as an Optical Bench Flexibility and modularity are core principles underlying Nikon’s imaging systems and components. In this presentation, we will discuss innovative solutions from Nikon that are designed to maximize flexibility and enable easy customization of both home-built microscopes and fully turn-key commercial systems. Learn how the Eclipse Ti2 with its expandable struc- ture, multiple input and output ports, and easy access to the back-aper- ture plane can be used as an optical bench. Furthermore, Nikon’s wide range of modular illumination devices from TIRF to photo-stimulation can be custom-combined for your unique application. For those requiring full access to the optical path, Nikon also offers the Ti2-Hardware Develop- ment Kit for introducing completely custom components. We will also discuss Nikon’s latest objectives utilizing new immersion media and featuring industry-leading numerical aperture and working distance. Speaker Ian Ross, Senior Biosystems Applications Manager, Nikon Instruments Inc Student Research Achievement Awards (SRAA) Poster Competition 3:30 pm-5:30 pm This poster competition features graduate and PhD students who are presenting posters at the Annual Meeting and have indicated at the time of abstract submission that they wish to participate in the competition. During the competition, students' one-to-three minute oral presentations of their posters will be reviewed by one or more judges, followed by a live question and answer period. Winners will be recognized on Friday prior to the Biophysical Society Lecture.

Exhibitor Presentation Horiba Scientific 4:00 pm - 4:30 pm Rapid, Optical Technique For Sensitive Characterization and Differentia- tion of OTC Canine Vaccines The pharmaceutical industry increasingly relies on spectroscopy for qual- ity assurance and has established, and conforms to, USP (United States Pharmacopeia) regulations. While some spectroscopic approaches (NIR, FT-IR and Raman) are common, the adoption of fluorescence spectros- copy has lagged, even though it has high specificity and sensitivity in many analyses in demand, and is conveniently amenable to chemomet- rics analysis. We present identification and validation of “unknown” samples with 100% certainty based on A-TEEM fluorescence analysis of Solo-Jec brand canine vaccines from Boehringer Ingelheim VetMedica: Spectra-5, Spec- tra-6, Spectra-9, and Spectra-10, containing 5, 6, 9 and 10 vaccine combi- nations, respectively of coronavirus, hepatitis, adenovirus, parainfluenze, leptospirae, parvovirus, etc. A key consideration upon an established vaccine product release is specificity. Analytical techniques need to char- acterize the final product, and must also differentiate between it and all others made at the facility. The 3-D fluorescence molecular fingerprints of these vaccines were subjected to chemometric analysis through PARA- FAC classification as well as XGBOOST discriminant analysis. The “most probable prediction” of unknown samples with 100% certainty was substantiated by the generated confusion matrix supporting the A-TEEM fluorescence claim to be a powerful addition to the arsenal of validation techniques. Two lots for each vaccine were measured on one instrument and were validated with a different lot, instrument and operator. The data analysis approaches used were each able to differentiate between the vaccine products. Even Spectra-9 and Spectra-10, that differ ONLY by a coronavirus component based on publicly available SDS data, were readily distinguished. Fluorescence EEMs (Excitation-Emission Matrix) solve the longstanding issue of imperfect quantification (a result of the Inner Filter Effect) by directly incorporating a UV spectrophotometer in the fluorometer. This allows the simultaneous acquisition in situ of a UV/VIS/NIR-absorbance spectrum for the real-time Inner Filter Effect (IFE) correction of the fluorescence spectrum, improving quantification accuracy and extend- ing the usable range of concentrations over which quantification can be performed. A-TEEM (Absorbance Transmission Excitation Emission Matrix) is fully validatable using United States Pharmacopeia monograph USP <853>, given that the novel aspect of simultaneous acquisition of the UV/VIS/NIR absorbance spectrum for IFE correction is fully compatible with validation protocols. This spectroscopic approach provides a com- plete and traceable optical fingerprint for liquid samples that performs a similar role to chromatographic methods, and compared to other spectroscopic methodologies is faster, less expensive and can operate in production environments. Speaker Karoly Csatorday, Business Development Manager, Horiba Scientific

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