Biophysical Society 65th Annual Meeting Program Guide

Platform Optical Microscopy and Superresolution Imaging II 10:00 am - 11:30 am Chair Thomas Surrey, Francis Crick Institute, United Kingdom 508-Plat 10:00 am MICROTUBULE NUCLEATION PROPERTIES OF SINGLE HUMAN ΓTURCS INVESTIGATED BY TIRF MICROSCOPY AND CRYO-ELECTRON MICROSCOPY. Tanja Consolati, Julia Locke, Johanna Roostalu, Zhuo Angel Chen, Julian Gannon, Jayant Asthana, Wei Ming Lim, Fabrizio Martino, Milos A. Cvet- kovic, Juri Rappsilber, Alessandro Costa, Thomas Surrey 509-Plat 10:30 am HYPERSPECTRAL ANALYSIS OF FLUORESCENCE IMAGES IN SCATTERING MEDIA USING OPTICAL FILTERS. Belén Torrado , Alexander Dvornikov, Enrico Gratton 510-Plat 10:45 am INFORMATION OPTIMAL PARTICLE TRACKING WITH A CONFOCAL OR 2-PHOTON MICROSCOPE. Nicholas A. Vickers , Fatemeh Sharifii, Samuel C. Pinto, Sean B. Andersson 511-Plat 11:00 am ASSESSING THE RELIABILITY OF THE SUPPOSE ENHANCED DECONVO- LUTION METHOD APPLIED TO DIFFERENT FLUORESCENT IMAGING TECHNIQUES. Micaela Toscani , Axel Lacapmesure, Guillermo D. Brinatti Vazquez, Oscar E. Martinez, Sandra R. Martinez 512-Plat 11:15 am NEW CHROMOPHORES FOR FRET-BASED VOLTAGE SENSING SYSTEMS. Ping Yan , Julia K. Harrison, Daniel Fairchild, Corey D. Acker, Leslie M. Loew Exhibits 10:00 am - 5:00 pm Exhibitor Presentation Bruker 11:30 am - 12:00 pm Bruker’s BioAFM Nano-Toolkit for Investigation of Mechanics, Struc- tures and Dynamic Processes in Life Science The ability of atomic force microscopy (AFM) to obtain three-dimensional topography images of biomolecules and complexes with nanometer reso- lution, and optical tweezers (OT) to measure sub-piconewton molecular forces under near-physiological conditions remains unmatched by other single molecule techniques. JPK BioAFM has developed the new NanoW- izard® 4 XP and NanoRacer AFM’s, and Nanotracker2 (NT2) optical twee- zers to set the bar even higher in these areas. The NW4XP is capable of high-speed study of the time-resolved dynamics associated with cellular processes; Nanoracer is designed for studying single molecule dynam- ics at 50 frames/sec; and NT2 can measure intra/inter molecular forces with multiple laser/trap options (up to 10 mW). With the latest scanner technologies inbuilt into NW4XP, NT2 and their compact design also al- lows full integration of AFM and OT into advanced commercially available light microscopy techniques. This seminar will focus on how the advances in Bruker’s latest BioAFM can be applied to study a wide-range of biologi- cal samples, from individual biomolecules to mammalian cells and tissues in real-time, in-situ experiments. We will present examples of how we are able to resolve the nanoscale structure of individual biomolecules at high-speed scan rates (150 - 5000 Hz), follow the dynamic reorganization of the membrane-associated cytoskeleton of living cells at high-temporal and high-spatial resolution, and automatically map the topography of cell cultures across the entire area of the microscope stage. We will also in-

troduce our new scanning electrochemical microscopy (SECM) module and discuss the full suite of BioAFM modes and accessories for studying the nanomechanical properties of cells and tissues, including direct cor- relation of multiparametric, quantitative AFM and super-resolution (STED)

W E

datasets. Speaker Samrat Dutta, Sales Applications Scientist, Bruker Break 11:30 am - 12:00 pm

D N

Global Pandemic Response Charting a Path Forward Using Guides from the Past and Present 12:00 pm - 1:30 pm A candid look at the global challenges facing a successful pandemic strategy. Can the lessons from other pandemics provide a framework for international cooperation? Can a strategy be developed that addresses both public health concerns and doubts in the general populace about the safety/efficacy of new interventions? Hear from global experts in sci- ence and policy as they weigh in on the challenge ahead. Chair Ranajeet Ghose, The City College of New York, USA Speakers Thurka Sangaramoorthy, University of Maryland, USA Patrick McTamney, AstraZeneca, USA Felix Rey, Pasteur Institute, France Arturo Casadevall, Johns Hopkins University, USA Platform Protein Assemblies 12:00 pm - 1:30 pm Chair Sheena Radford, University of Leeds, United Kingdom 513-Plat 12:00 pm BUILDING THE BACTERIAL CELL WALL: HOW DO BACTERIA DO IT? Sheena Radford 514-Plat 12:30 pm EXPLORING THE MORPHOLOGICAL LANDSCAPE OF SELF-ASSEMBLED HIV-1 PROTEINS FROM A COARSE-GRAINED PERSPECTIVE. Alexander J. Pak , Gregory A. Voth 515-Plat 12:45 pm LOCALIZATION TO A MEMBRANE SURFACE CAN ACCELERATE THE PRO- TEIN ASSEMBLIES. Bhavya Mishra , Margaret E. Johnson 516-Plat 1:00 pm IN VITRO RECONSTITUTIONS AND FUNCTIONAL CHARACTERIZATIONS OF FIBRILLAR CENTERS OF NUCLEOLI. Matthew R. King , Rohit V. Pappu 517-Plat 1:15 pm KINETIC AND EQUILIBRIUMMEASUREMENT OF NUCLEATION OF PRION- LIKE DOMAIN PHASE SEPARATION. Erik W. Martin , Tyler S. Harmon, Jesse B. Hopkins, Srinivas Chakravarthy, Jeremias Incicco, Peter Schuck, Andrea Soranno, Tanja Mittag

E S

D A Y

42