Biophysical Society 65th Annual Meeting Program Guide

Platform Other Channels and Regulatory Mechanisms 12:00 pm - 1:30 pm Chair Sara Liin, Linköping University, Sweden 535-Plat 12:00 pm RATIONAL DESIGN OF FATTY ACID ANALOGUES TARGETING THE CARDIAC KV7.1 CHANNEL. Sara I. Liin 536-Plat 12:30 pm K 2P CHANNEL C-TYPE GATING INVOLVES ASYMMETRIC SELECTIVITY FILTER ORDER-DISORDER TRANSITIONS. AndrewM. Natale , Marco Gaetano Lorenz Lolicato, Fayal Abderemane-Ali, David Crottès, Sara Capponi, Ra- mona Duman, Armin Wagner, John M. Rosenberg, Michael Grabe, Daniel 12:45 pm TRPV2 MODULATION BY SMALL MOLECULES AND LIPIDS. Anna D. Protopopova , Ruth A. Pumroy, Jeanne de la Roche, Ferdinand M. Haug, Bárbara B. Sousa, Pamela N. Gallo, Gonçalo J.L. Bernardes, Andreas Lef- fler, Vera Y. Moiseenkova-Bell 538-Plat 1:00 pm CHLORIDE PERMEATION THROUGH THE CYSTIC FIBROSIS TRANSMEM- BRANE CONDUCTANCE REGULATOR (CFTR): A MOLECULAR SIMULATION STUDY. Zhi Wei Zeng , Régis Pomès 539-Plat 1:15 pm STRUCTURAL AND FUNCTIONAL INVESTIGATION ON HUMAN PANNEXIN 1 (PANX1) REVEALS NOVEL INSIGHT INTO CHANNEL GATING, ION PERME- ATION AND DRUG INHIBITION. Zheng Ruan , Orozco J. Ian, Juan Du, Wei Lu Platform Member Organized Session: Pattern Formation and Biochemical Excitability in Controlling Cell System Dynamics 12:00 pm - 1:30 pm Co-chairs Wolfgang Losert, University of Maryland College Park, USA Tatsat Banerjee, Johns Hopkins University, USA 540-Plat 12:00 pm EXCITABLE NETWORKS IN DIRECTED CELL MIGRATION. Peter N. Devreotes , Tatsat Banerjee, Dhiman S. Pal, Debojyoti Biswas, Huiwang Zhan, Pablo A. Iglesias 541-Plat 12:18 pm CYTOSKELETAL DYNAMICS AND MECHANOSENSING IN IMMUNE CELLS. Arpita Upadhyaya 542-Plat 12:36 pm DIFFERENT CELL MIGRATION MODES: INSIGHTS FROM TRACTION FORCE MICROSCOPY AND MODELING. Wouter-Jan Rappel , Elisabeth Ghabache, Yuansheng Cao, Yuchuan Miao, Alexander Groisman, Peter N. Devreotes 543-Plat 12:54 pm PHYSICAL CONTROL OF INTRACELLULAR WAVES WITH NANOTOPOGRA- PHY AND ELECTRIC FIELDS. Qixin Yang , Matt Hourwitz, Leonard Campan- ello, Peter N. Devreotes, John Fourkas, Wolfgang Losert 544-Plat 1:12 pm PULSATILE DYNAMICS OF EGFR/RAS/ERK SIGNALING: ROLES IN EPITHE- LIAL PHYSIOLOGY AND DISEASE. John Albeck L. Minor 537-Plat

Platform Single-Molecule Spectroscopy 12:00 pm - 1:30 pm

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Chair Gilad Haran, Weizmann Institute of Science, Israel 545-Plat 12:00 pm UNDERSTANDING MICROSECOND DYNAMICS OF PROTEIN MACHINES. Gilad Haran 546-Plat 12:30 pm DIRECT DIGITAL SENSING OF PROTEINS IN SOLUTION THROUGH SINGLE- MOLECULE OPTOFLUIDICS. Georg Krainer , Kadi Liis Saar, William E. Arter, Tuomas P.J. Knowles 547-Plat 12:45 pm ROTATION TRACKING USING DNA ORIGAMI ROTORS REVEALS SINGLE BASE PAIR STEPS DURING TRANSCRIPTION BY RNA POLYMERASE. Pallav Kosuri , Benjamin D. Altheimer, Mingjie Dai, Peng Yin, Xiaowei Zhuang 548-Plat 1:00 pm TUNING THE BAIRD AROMATIC TRIPLET STATE ENERGY OF CYCLOOCTA- TETRAENE TO MAXIMIZE THE SELF-HEALING MECHANISM IN ORGANIC FLUOROPHORES. Avik K. Pati , Ouissam El Bakouri, Steffen Jockusch, Zhou Zhou, Roger B. Altman, Gabriel A. Fitzgerald, Wesley B. Asher, Daniel S. Terry, Alessandro Borgia, Jonathan A. Javitch, Henrik Ottosson, Scott C. Blanchard 549-Plat 1:15 pm PROTEIN CONFORMATIONAL DYNAMICS IN CELLULA AND IN VIVO USING LIVE-CELL SINGLE-MOLECULE FRET HILO MICROSCOPY. Abhinaya Anandamurugan , Philipp Wortmann, Chenyang Lan, April Garcia Fer- nando, Marina Veil, Lukas Schrangl, Ritwick Sawarkar, Wolfgang Driever, Gerhard J. Schuetz, Thorsten Hugel General Networking 1:00 pm-2:00 pm Catch up with colleagues and friends in the biophysical community during our networking hour. This event, which is open to all meeting attendees, provides meet-and-greet opportunities to enhance your virtual meeting experience. BPS Virtual Education and Career Fair 1:00 pm - 4:00 pm Learn about the different leading biophysics programs and career op- portunities. This virtual fair will give you the opportunity to speak to representatives from different institutions and companies about their biophysics programs and career opportunities. All those attending the Annual Meeting are encouraged to attend. Exhibitor Presentation Leica Microsystems 1:30 pm - 2:00 pm High Photon-Count Rate FCS and STED-FCS to Study Diffusion Dynamics in Live Cells Investigating diffusion dynamics of proteins and small molecules has be- come a routine measurement all across the life sciences, chemistry and physics. It provides valuable insights into reaction dynamics, oligomeriza- tion, molecular interactions or cellular (membrane) heterogeneities [1] on the other hand, routinely accessible by spectroscopic techniques such as fluorescence correlation spectroscopy (FCS. Fluorescence correlation spectroscopy (FCS) is a versatile tool to determine diffusion dynamics in membranes (2D diffusion) and solution or the cytoplasm (3D diffusion). Measuring the intensity fluctuations over time due to the diffusion of mol- ecules through the observation volume is the basis for FCS. Temporal au- tocorrelation of the signal allows for the calculation of the autocorrelation

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