Biophysical Society 66th Annual Meeting Program Guide

Esplanade, Room 157: Tuesday, February 22 9:30 AM – 11:00 AM Sophion Bioscience A/S Use of iPSC-Derived DRG Neurones on Sophion Bioscience Systems. Screening For Sodium Channel and KCa Modula- tors and Positive Allosteric Modulators For GABA Channels. Development of Assays For Novel Targets on the Qube. Successful ion channel drug discovery requires the integration of mul- tiple technologies and workflows. Sophion Bioscience is a leader in automated patch clamp technology, providing low, medium and high throughput patch clamp systems to the drug discovery industry and aca- demia. The QPatch and Qube are fully automated patch clamp systems, executing simultaneous 16, 48 or 384 parallel patch clamp recordings in conjunction with computer controlled liquid handling and on-board cell handling. The QPatch Compact is a manual patch clamp system that can execute individual or simultaneous 8 patch clamp recordings. Sophion provides customers with robust, ion channel and electrophysiological workflows for drug development of ion channel targets. During this workshop, three industry speakers will provide insight into the use of these systems in the drug discovery process. Dr Daniel Sauter will pres- ent automated patch clamp data to show use of iPSC-derived neurons on Sophion Bioscience systems. Professor Heike Wulff will present data and discuss screening for sodium channel and KCa modulators and positive allosteric modulators for GABA channels. Dr Adam Hyman will present data on novel lysosomal and mechanically activated ion channel targets on the Qube automated patch clamp platform. Speakers Adam Hyman, Senior Scientist, Charles River Laboratories Daniel Sauter, Scientific Sales Manager, Sophion Bioscience A/S Heike Wulff, University of California, Davis 11:30 AM – 1:00 PM LUMICKS Unlocking Higher Impact Science With the New C-Trap: A Dedicated Dynamic Single-Molecule Tool For Cytoskeletal Surface Assays In order to obtain a full understanding of the fundamental biological processes in the cell, scientists need to have insights into the molecular mechanisms involved. Studying cytoskeletal structure and transport, therefore, requires information on the mechanical properties and dynamics of molecular motors and filaments at the single-molecule level. Not having this information often leads to an incomplete under- standing of the molecular processes involved and less impactful sci- ence. The new LUMICKS C-Trap product line enables scientists to obtain a thorough understanding of cytoskeletal structure and transport – and do this much faster and more reliably than before. Here, we present our latest developments on the LUMICKS C-Trap that have unlocked dynamic single-molecule analysis in this scientific field and are enabling higher impact studies. The optical tweezers have now been fully optimized for near-surface experiments. The highest force resolution (3D) and stability enable measuring the smallest motor steps, most precise loads of cytoskeletal motors, and filament bending. This is achieved by adding functionalities such as active surface stabilization, accurate near-surface force cali- bration, axial force detection, and surface assay force feedback.

5:30 PM – 7:00 PM Elements srl The World’s First Commercial 10 MHz Bandwidth and Low- Noise Amplifier For Nanopore Applications Ultra-portable and cost-effective amplifier technology is now a reality, accessible to any research laboratory thanks to Elements miniaturized products based on custom CMOS microchips. Our flagship products are the eNPR, a handheld nanopore kit for nanoparticle detection using dis- posable glass nanopore chips and the new 10 MHz bandwidth amplifier for nanopore applications. The 10 MHz amplifier can boast an excep- tional low-noise on the whole spectrum up to 10 MHz, a stunning time resolution of 25 ns without nanopore chips and excellent EMI shielding thanks to the integrated faraday cage. In this presentation, current collaborators and customers from the US and Europe will be featuring our latest products through their own expe- riences, ranging from nanopore and nanopipettes experiments at high bandwidth. Also, you will learn how to measure translocations through nanopores of environmental samples and the application of nanopi- pettes in analytical science. Presentation agenda: ● Company overview, Federico Thei ● “The NASA project sequel, is there life on other bodies of our solar system? Detecting life in Ocean Worlds with low-capaci- tance solid-state nanopores”, David Niedzwiecki ● 10 MHz Bandwidth amplifier product launch, Federico Thei ● “Preliminary results and comparison to previous translocation data at 10 MHz”, David Niedzwiecki, Marija Drndi ć ● “Nanopipettes high bandwidth experiments”, Samuel Confederat About Elements: Elements provides versatile solutions to measure electric currents in the picoampere (10-12 pA) range, with bandwidths up to hundreds of kHz, featuring very low noise recordings, signal digitization thanks to the integrated Analog-to-Digital converter, signal generator, digital data elaboration, and USB powered, all in a tiny form factor, about the size of a point-and-shoot digital camera! Complimentary Italian hors d’oeuvres and drinks will be served! Seating

is limited! Speakers

Federico Thei, CEO Elements srl David Niedzwiecki, Goeppert LLC Marija Drndi ć , Professors of Physics, University of Pennsylvania Samuel Confederat, University of Leeds

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