Biophysical Society 66th Annual Meeting Program Guide

Symposium Structure-Function of Long Non-Coding RNAs 4:00 pm - 6:00 pm, South, Level Three, Room 303/304 Chair Mitchell Guttman, California Institute of Technology, USA 807 -Symp 4:00 pm G QUADRUPLEX STRUCTURES IN NEAT1: POTENTIAL FUNCTIONS IN REGULATING ITS STABILITY AND ITS INTERACTIONS WITH THE FUSED IN SARCOMA PROTEIN. Mihaela-Rita Mihailescu , Emily M. Benner, Caylee Cunningham, Carlan Grey, Ella Milback 808 -Symp 4:30 pm DYNAMIC INTRAMOLECULAR LNCRNA TRIPLEXES: TRANSCRIPT STABIL- ITY ELEMENTS AND SMALL MOLECULE TARGETS. Abeer Ageeli, Monalisa Swain, Michael J. Yonkunas, Wojciech Kasprzak, Kayleigh McGovern- Gooch, Bruce A. Shapiro, Stuart F.J. Le Grice, Nathan Baird 809 -Symp 5:00 pm THE MULTIPLE DIMENSIONS OF LNCRNAS: HOW 3D STRUCTURE DETER- MINES MEG3 FUNCTION. Marco Marcia 810-Symp 5:30 pm HOW NON-CODING RNAS SHAPE NUCLEAR STRUCTURE TO REGULATE GENE EXPRESSION. Mitchell Guttman Platform Protein Assemblies 4:00 pm - 6:00 pm, South, Level Two, Room 201/202/203 Co-Chairs Lauren Ann Metskas, California Institute of Technology, USA Alex Vecchio, University of Nebraska-Lincoln, USA 811-Plat 4:00 pm FILAMENT ASSEMBLY REGULATES THE CATALYTIC ACTIVITY OF PHOSPHO- RIBOSYL PYROPHOSPHATE SYNTHETASE 1. Kelli L. Hvorecny , Kenzee A. Hargett, Joel Quispe, Justin M. Kollman 812-Plat 4:15 pm LC8 DRIVES HIGHER ORDER OLIGOMERIZATION IN 53BP1. Jesse J. Howe , Patrick N. Reardon, Elisar J. Barbar 813-Plat 4:30 pm INFLUENCE OF ASSEMBLY MODULATORS ON THE ASSEMBLY KINETICS AND STRUCTURE OF HEPATITIS B VIRUS CAPSID. Kalouna Kra , Laetitia Gargowitsch, Stephane Bressanelli, Guillaume Tresset 814-Plat 4:45 pm CRYO-EM STRUCTURES OF HUMAN CLAUDIN-4 IN COMPLEX WITH ITS BACTERIAL TOXIN ANTAGONIST ENABLED BY SYNTHETIC ANTIBODY FRAGMENTS REVEAL TARGETING MECHANISMS AND THERAPEUTIC PO- TENTIAL. Benjamin J. Orlando, Pawel K. Dominik, Sourav Roy, Chineme- rem Ogbu, Satchal K. Erramilli, Anthony A. Kossiakoff, Alex J. Vecchio 815-Plat 5:00 pm HEPATITIS B VIRAL CAPSID DISASSEMBLY AND GENOME SYNTHESIS: FROM THE ATOMIC LEVEL TO THE WHOLE CELL. Zhaleh Ghaemi , Martin Gruebele, Emad Tajkhorshid 816-Plat 5:15 pm BACTERIAL MICROCOMPARTMENT ORGANIZATION BY CRYO ELECTRON TOMOGRAPHY AND HIGH-RESOLUTION SUBTOMOGRAM AVERAGING. Lauren Ann Metskas , Davi Ortega, Luke M. Oltrogge, Cecilia Blikstad, David Savage, Grant J. Jensen 817-Plat 5:30 pm CS20 BRIDGE THE GAP BETWEEN CLASS 1 AND CLASS 5 BACTERIAL ADHESION PILI. Matthew Doran, Tobias Dahlberg, Joseph Baker, Magnus Andersson, Esther Bullitt

microenvironment but often fail, suffering from lack of reproducibility and incompatibility with other well-established end-point assays. Here, we demonstrate biomimetic in vitro platforms capable of reliably reproducing these essential cues. These platforms markedly improve the structural and functional development of a variety of cell types, including stem cells, cardiomyocytes, muscle cells, and more. These cutting-edge strategies can be deployed in both 2D and 3D model systems for high- throughput assessment of metabolism, electrophysiology, and contractil- ity. We describe how the differentiation of stem cells can be enhanced by providing a more biomimetic culture environment, with a particular focus on iPSC-derived cardiomyocytes and skeletal muscle cells. Further, we highlight how an active mechanical environment combined with aligned cell-nanotopography cues improves adhesion, signaling, and polar- ity across many cell applications. Finally, we demonstrate how to scale these technologies to 3D organoid systems, and how these approaches can improve the development of in vitro disease models to support the discovery of new therapies. Speaker Hamed Ghazizadeh, Product Manager, Curi Bio Membership Committee Meeting 3:30 pm - 5:30 pm, South, Level Three, Room 312 Career Development Center Workshop Marketing Your Value: Crafting Your Elevator Pitch/30 Second Value Statement/Brand Statement 4:00 pm - 5:00 pm, Esplanade, Room 151 In this workshop, we will address how to effectively market yourself for career opportunities (academia, industry and beyond) and leverage and utilize marketing methods and channels, and we will spend time on how to craft an elevator pitch and what makes a great pitch for employers. Symposium Membrane Tension 4:00 pm - 6:00 pm, South, Level Three, Room 301/302 Chair Padmini Rangamani, University of San Diego, USA 804 -Symp 4:00 pm MECHANICAL SIGNALING AT THE CELL MEMBRANE. Zheng Shi , Shilong Yang No Abstract 4:30 pm PLASMA MEMBRANE TETHERING CONTROLS CORTEX MECHANICS AND ARCHITECTURE. Alba Diz-Munoz 805-Symp 5:00 pm IN VITRO MORPHOGENESIS BY CELLULAR TORNADOES. Pau Guillamat, Carles Blanch-Mercader, Guillaume O. Pernollet, Karsten Kruse, Aurelien Roux 806-Symp 5:30 pm ELUCIDATING THE ROLE OF MEMBRANE TENSION IN CELLULAR PROCESS- ES USING CONTINUUMMODELING. Padmini Rangamani

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