Biophysical Society 67th Annual Meeting Program Guide

11:30 AM – 1:00 PM Sophion Bioscience A/S

5:30 PM – 7:00 PM Elements srl Nanopore Measurements at 10 MHz Bandwidth: Let’s See the Hidden Data You Were Looking For! From its introduction in February 2022 of the new 10 MHz bandwidth Nanopore Reader, several labs have started to use our new powerful instrument, featuring unprecedent results and signal quality in high bandwidth nanopore measurements. We will present data from a recent work of Prof. Marija Drndic about ultrafast translocation and dynamics of double stranded DNA through a nanopore at 10 MHz bandwidth with acquisition of data points per 25 nanoseconds (150 MB/s). By introducing a rigorous algorithm, it was accurately identified each current level present within events. Remarkable sensitivity of this system reveals distortions of short-lived states at a lower bandwidth. This work develops broadly applicable methods that will help uncover a wide range of biomolecular dynamics at unprecedentedly small timescales. 10 MHz bandwidth allows to observe protein conformation and kinetics never observed before: here we present the latest results from protein measurements. Speakers Marija Drndic, Professors of Physics, University of Pennsylvania Federico Thei, CEO, Elements srl Room 9: Tuesday, February 21 9:30 AM – 11:00 AM Bruker Single-Molecule Localization Microscopy for Advanced Biological and Genomic Imaging in Three Dimensions: The Bruker Vutara VXL Most sub-cellular structures are smaller than 300 nm, beyond the opti cal resolution limit for fluorescence microscopy. The single-molecule localization microscope (SMLM) Bruker Vutara VXL overcomes this limi tation. With an optical resolution of 20 nm, SMLM is uniquely qualified to address biological mysteries that require specific labeling as used in fluorescence microscopy but higher resolution than can be achieved with diffraction-limited microscopy. In addition to SMLM, the Vutara VXL implements imaging workflows for multiplexed chromatin tracing and smFISH applications. The system images, resolves, and quantifies cellular structures, molecular machines, proteins, RNA, and chromo somal structures. SMLM modulates the emission from dye molecules to prevent their point-spread-function (PSF) spatial overlap. Advanced fitting algorithms localize the position of each dye molecule with a resolution well below the optical resolution limit. The proprietary bi-plane technology of the Bruker Vutara VXL extends the traditional 2D fitting into the third dimension, far away from the coverslip. In STORM and PALM, a laser switches dye molecules actively on and off. Special imaging buffers create a similar blinking spontaneously in dSTORM. DNA-PAINT relies on oligonucleotides’ transient binding, and genomic applications use sparse labeling and multiplexing. In this presentation, we will discuss how the Bruker Vutara VXL com pact bench-top system brings the power of SMLM into your lab. Speaker Winfried Wiegraebe, Product Manager Super-Resolution Microscopy, Bruker

Electrophysiological Characterization of Cardiac Myocytes Using Sophion Bioscience’s Automated Patch Clamp Sys tems and the Intracellular Side of Axxam Electrophysiology Successful ion channel drug discovery requires the integration of mul tiple technologies and workflows. Sophion Bioscience is a leader in automated patch clamp technology, providing low, medium and high throughput patch clamp systems to the drug discovery industry and academia. The QPatch and Qube are fully automated patch clamp systems, executing simultaneous 16, 48 or 384 parallel patch clamp recordings in conjunction with computer controlled liquid handling and on-board cell handling. The QPatch Compact is a manual patch clamp system that can execute individual or simultaneous 8 patch clamp recordings. Sophion provides customers with robust, ion channel and electrophysiological workflows for drug development of ion channel targets. During this workshop, industry speakers will provide insight into the use of these systems in the drug discovery process. Dr Daniel Sauter will present automated patch clamp data to show the electrophysiolog ical characterization of cardiac myocytes using automated patch clamp on Sophion Bioscience systems. Dr Manuel Paina will present data on the use of Sophion to research intracellular ion channel targets. Speakers Daniel Sauter, Scientific Sales Manager, Sophion Bioscience A/S Manuel Paina, Organellar Electrophysiology Supervisor, Axxam S.p.A 1:30 PM – 3:00 PM Delmic Streamline the Cryo-ET Workflow and Get More Useful Bio logical Insights with Delmic’s METEOR and CERES Systems Cryo-ET is a powerful imaging technique that allows protein complexes in their near-native cellular environment to be resolved at a nanome tre resolution. Unfortunately the uptake of this powerful technique is hampered by its current error prone workflow, which often results in damaged, contaminated, and devitrified samples that cannot be used for data analysis. At Delmic we developed a host of technologies to unleash cryo-ET’s full potential. We will present METEOR, a fluorescent light microscope that is directly integrated into a cryo-FIB/SEM. It allows the user to skip unnecessary transfer steps and therefore decrease ice contamination. The presence of the targeted fluorescent signal in the lamellae can be checked during and after the milling process and cryo-CLEM data quality is improved. METEOR can therefore dramatically increase cryo-ET sample yield. We will also present the CERES Ice Defence System, which increases sample throughput and quality by minimizing ice contamination throughout the cryo-ET sample preparation workflow. We will show that the samples can be prepared contamination free in the CERES Clean Station, a dedicated and moisture free workstation for cryo-ET sample preparation. From this workstation the samples can be trans ferred to the cryo-FIB/SEM using the CERES Vitri-Lock providing a high vacuum, anhydrous environment. In the FIB/SEM the sample is pro tected by the CERES Ice Shield, which protects against amorphous ice build-up on the sample. This protection is crucial for high cryo-ET data quality and allows long automated milling sessions. Delmic’s cryo solutions allow researchers to obtain better quality cryo ET results, higher throughput, and higher resolution. Speaker Marit Smeets, Product Manager, Delmic

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