Biophysical Society 67th Annual Meeting Program Guide

Finally, Dr. Koenig and Dr. Sisamakis will describe how fluorescence lifetime imaging (FLIM) is streamlined with Luminosa. Its rapidFLIM hardware can record several frames per sec with very high photon count rates. The software handles these with a novel dynamic binning format. In combination with GPU-accelerated algorithms, this enables high-speed analysis of FLIM images. The automated analysis algorithm suggests the best model for multiexponential fitting, calculates a pha sor plot and offers pattern matching. A lot of expert knowledge went into the design of Luminosa. This empowers researchers to confidently explore new paths with time resolved fluorescence techniques. Speakers Evangelos Sisamakis, Product Manager Microscopy, PicoQuant GmbH Marcelle Koenig, PicoQuant GmbH 2:30 PM – 4:00 PM Oxford Instruments Multi-Modal Microscopy: Challenges and Workflows for Correlative Microscopy Acquisition and Analysis Correlative microscopy data is beautiful and incredibly complex, par ticularly when dealing with data acquired at significantly different magnification scales, 3D data, data acquired using analytical techniques or dynamic data that has been acquired at multiple time points with more than one type of imaging technique. Combining multiple micros copy techniques can have more than one purpose, enabling research ers to better target regions of interest and optimize microscope and researcher time, and also to enable a deeper insight into the structural and functional properties of your sample. Where do you begin with multi-modal microscopy? This interactive workshop will take you through some of the consid erations for biological microscopists when approaching correlative microscopy, including sample preparation, how to find your regions of interest, and how to determine the sequence of microscopy tech niques. We will also provide case studies using a range of techniques for correlative microscopy research on biological samples, including analytical techniques that go beyond structural imaging; Raman spec troscopy, atomic force microscopy (AFM), electron microscopy, and energy dispersive x-ray spectrometry (EDS). How do you analyze the data? Collecting the data is only the start, a significant proportion of research time is spent on data collation and interpretation. The final part of this workshop will discuss techniques to process correlative data and optimize your analysis, taking you beyond image overlays through to producing quantitative, analytical results. Speaker Louise Hughes, Business Manager, Oxford Instruments

Room 10: Sunday, February 19 10:30 AM – 12:00 PM Carl Zeiss Microscopy LLC

Discover How Accessible Lattice Light Sheet Technology and Image Analysis Really are with ZEISS Lattice Lightsheet 7 In order to best understand the world around us it is necessary to observe microscopic specimens in as natural a state as possible. This requires a transition from imaging fixed to live specimens and expand ing from 2D to 3D model organisms. The drive towards live-cell imaging over long timeframes and at high volume speeds brings new chal lenges. There is evidence that traditional imaging techniques can influ ence the behavior of specimens due to phototoxicity, thus affecting the integrity of the results, and with the push to perform long-term volu metric imaging comes a need to analyze increasingly larger data sets. This talk will describe how ZEISS Lattice Lightsheet 7 makes long-term volumetric imaging of living cells with subcellular resolution possible without having to change your standard sample preparation protocols to accommodate the instrument. With automatic alignment and easy acquisition workflows, lattice light-sheet imaging is now as accessible as using a standard inverted microscope. ZEISS ZEN microscopy soft ware and arivis Vision4D provide analysis pipelines which can easily be utilized to go from large image datasets to results. Join us for this presentation to learn how accessible lattice light sheet technology and image analysis really are with ZEISS Lattice Lightsheet 7, ZEN microscopy software and arivis Vision 4D. Speaker Renée Dalrymple, Life Science Business Sector Marketing Manager, Carl Zeiss Microscopy LLC 12:30 PM – 2:00 PM PicoQuant Photonics North America Inc Quantitative, Reproducible Fluorescence Microscopy Made Easy Quantitative single molecule and time-resolved fluorescence tech niques offer new insights into many samples in life and materials sci ences. So far, their adoption has been slow because expert knowledge was required for correct data acquisition and analysis. Now, PicoQuant developed a new single photon counting confocal microscope, called Luminosa. It combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. The system software includes context-based workflows for each tech nique, which improve accuracy and reproducibility of experiments. Advanced integration of hardware and software enables new features such as sample-free auto-alignment, excitation laser power calibration, and automatic configuration of hardware parameters for time-resolved measurements. These features make experiments more efficient and reliable. Dr. Koenig and Dr. Sisamakis will present how Luminosa brings single molecule Förster Resonance Energy Transfer (smFRET) experiments to a new level. For example, FRET efficiency (E) and stoichiometry (S) are calculated online, corrected according to the standard procedure of the community, and displayed live in an E/S histogram during the measure ment.

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