Biophysical Society 67th Annual Meeting Program Guide

Workshop Writing Award Nomination and Support Letters 1:00 pm - 2:30 pm, Room 6D Helping your colleagues, mentors, and mentees be recognized via awards, promotions, or hires is an important skill for everyone. If you choose a career in research and/or education, you will likely be asked to write many such letters. In this workshop, Biophysical Society Fellows will discuss what makes an effective letter. For practice, we recommend you come with a candidate in mind for one of the BPS awards. Effective nominations can come from scientists at any career stage! Chair Susan Marqusee, University of California, Berkeley, USA Education and Career Opportunities Fair 1:00 pm - 3:00 pm, Exhibit Hall BC Come to this fair to meet with representatives from educational institutions as well as industry and government agencies. Students and postdoctoral can didates will be able to meet with representatives from colleges and universi ties with leading programs in biophysics. Attendees can connect with repre sentatives from industry and agencies who will provide information about employment and funding opportunities at their institutions/companies. Learn about the variety of opportunities available and to talk one-on-one with representatives from participating organizations! Exhibitor Presentation Mad City Labs Inc 1:30 pm - 3:00 pm, Room 9 Open and Flexible Microscopy Systems for Combined Single-Molecule Methods Speaker: Eric Drier, Senior Scientist, Mad City Labs Inc Open and flexible microscopy systems have distinct advantages for imple menting novel biophysical methods. This session focuses on 3 such systems, each of which combines precision motion control with single molecule measurements. Quantification of Lipid Diffusion Dynamics on Live Cell Membranes through Interferometric Scattering Microscopy Speaker: Francesco Reina, Leibniz-Institut für Photonische Technologien e.V. and Institute of Applied Optics and Biophysics, Friedrich Schiller University Jena, Germany The study of single lipid dynamics on membranes requires simultaneously high localization precision and temporal resolution, a feat that few micros copy techniques are able to achieve. We show how Single Particle Tracking through Interferometric Scattering (ISCAT) microscopy has given us new insights in the compartmentalization of plasma membrane structure, and the diffusion modes of single, gold-nanoparticle tagged lipids. The tracked lipids appear to diffuse through a “hopping” motion between compartments of nanoscopic size (100-120nm) with a probability of crossing the boundary. These results were confirmed with the use of particle diffusion simulations in a corralled, two-dimensional plane, which also serve to estimate the “hop ping” probability. A Single-Molecule View on Dynamic Chromatin Access of Epigenetic Regulatory Factors Speaker: Beat Fierz, École Polytechnique Fédérale de Lausanne, Switzerland The dynamic organization of the eukaryotic genome into chromatin is integral to its regulation. We develop single-molecule colocalization imaging and single-molecule FRET approaches to directly observe the dynamic architec ture of chemically defined chromatin fibers. We found that local chromatin undergoes structural fluctuations on the micro- to millisecond timescale. Internal chromatin dynamics are exploited by transcription factors to invade chromatin structure. Using single-molecule imaging, we recently revealed DNA access mechanisms for pioneer TFs, genome editors and centromeric proteins. Overall, our results show that compact chromatin structure hinders factor access, but mechanisms that open nucleosome contacts, including his -

Career Development Center Workshop Looking Beyond Academia: Identifying Your Career Options using MyIDP, LinkedIn and More 12:00 pm - 1:00 pm, Room 16B Not sure where your professional future lies or how to approach the process in an organized and strategic manner? This presentation provides a framework and resources for moving forward with confidence towards the next step in your professional future. In addition, it will provide specific examples of how to build out your knowledge of a new potential career field and forge valuable connections that can facilitate a successful transition. Public Affairs Committee Meeting 12:00 pm - 1:30 pm, Room 14A BPS/IOP Advisory Board Meeting 12:00 pm - 4:00 pm, Room 17B Exhibitor Presentation PicoQuant Photonics North America Inc 12:30 pm - 2:00 pm, Room 10 Quantitative, Reproducible Fluorescence Microscopy Made Easy Quantitative single molecule and time-resolved fluorescence techniques offer new insights into many samples in life and materials sciences. So far, their adoption has been slow because expert knowledge was required for correct data acquisition and analysis. Now, PicoQuant developed a new single photon counting confocal microscope, called Luminosa. It combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. The system software includes context-based workflows for each technique, which improve accuracy and reproducibility of experiments. Advanced integration of hardware and software enables new features such as sample-free auto-alignment, excitation laser power calibration, and automatic configuration of hardware parameters for time-resolved measurements. These features make experiments more efficient and reliable. Dr. Koenig and Dr. Sisamakis will present how Luminosa brings single molecule Förster Resonance Energy Transfer (smFRET) experiments to a new level. For example, FRET efficiency (E) and stoichiometry (S) are calculated online, corrected according to the standard procedure of the community, and displayed live in an E/S histogram during the measure ment. Finally, Dr. Koenig and Dr. Sisamakis will describe how fluorescence lifetime imaging (FLIM) is streamlined with Luminosa. Its rapidFLIM hardware can record several frames per sec with very high photon count rates. The software handles these with a novel dynamic binning format. In combination with GPU-accelerated algorithms, this enables high-speed analysis of FLIM images. The automated analysis algorithm suggests the best model for multiexponential fitting, calculates a phasor plot and of fers pattern matching. A lot of expert knowledge went into the design of Luminosa. This em powers researchers to confidently explore new paths with time-resolved fluorescence techniques. Speakers Evangelos Sisamakis, Product Manager Microscopy, PicoQuant GmbH Marcelle Koenig, PicoQuant GmbH

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