Biophysical Society 67th Annual Meeting Program Guide

regions of interest and optimize microscope and researcher time, and also to enable a deeper insight into the structural and functional proper ties of your sample. Where do you begin with multi-modal microscopy? This interactive workshop will take you through some of the consider ations for biological microscopists when approaching correlative micros copy, including sample preparation, how to find your regions of interest, and how to determine the sequence of microscopy techniques. We will also provide case studies using a range of techniques for correlative microscopy research on biological samples, including analytical tech niques that go beyond structural imaging; Raman spectroscopy, atomic force microscopy (AFM), electron microscopy, and energy dispersive x-ray spectrometry (EDS). How do you analyze the data? Collecting the data is only the start, a significant proportion of research time is spent on data collation and interpretation. The final part of this workshop will discuss techniques to process correlative data and optimize your analysis, taking you beyond image overlays through to producing quantitative, analytical results. Speaker Louise Hughes, Business Manager, Oxford Instruments Early Careers Committee Meeting From Immunology to Ion Channels: Microfluidic Approaches to Automated Patch Clamp and Cellular Analysis. A Look at IonFlux Mercury and BioFlux Systems from Fluxion Biosciences. Leveraging proprietary microfluidic approaches, Fluxion Biosciences provides unique solutions that simplify and automate complex cell-based assays. This presentation will cover two Fluxion systems used extensively in biophysical characterization of cells: IonFlux and BioFlux. IonFlux Mercury automated patch clamp (APC) systems are capable of recording from 16 to 256 separate cells simultaneously in whole cell patch clamp mode. With unique features such as industry-leading fast in plate compound exchange and continuous solution flow, IonFlux systems are used globally in pharma and academic laboratories for both ligand and voltage-gated ion channel screening. Recent developments include automated IC/EC50 calculation workflows, and the introduction of a new range of GABA cells for complete sample-to-result analysis. The BioFlux system is the world’s leading platform for analyzing cell-cell interactions in a flow-controlled environment. Applications include characterization of cell migration, adhesion strength, transmigration, and chemotaxis. BioFlux assays for research and drug development in im munology, vascular biology, and cancer will be reviewed. Recent research in covid-induced thrombocytopenia and functional analysis of CAR-T cells will be highlighted. Speakers Jeff Jensen, Chief Executive Officer, Fluxion Biosciences Ali Yehia, Chief Scientific Officer, Fluxion Biosciences Career Development Center Workshop Nailing the Job Talk, or Erudition Ain’t Enough 4:00 pm - 5:00 pm, Room 16B Congratulations! You’ve made it to the finals and are suddenly facing the most important presentation of your life. Answers to your questions about how to structure your presentation, how much detail to include, what they are really looking for, etc. 3:30 pm - 5:00 pm, Room 14A Exhibitor Presentation Fluxion Biosciences 3:30 pm - 5:00 pm, Room 9

tone variants and chromatin remodeling complexes can help key factors to overcome these obstacles. Magnetic Tweezers Investigations of the Type IA Topoisomerase of Mycobacterium Smegmatis Speaker: Maria Mills, Department of Physics and Astronomy, University of Missouri Type IA topoisomerases relieve torsional strain in supercoiled DNA. These enzymes work by cleaving the backbone of one strand of a DNA duplex, passing the other strand through the break, and re-ligating the DNA. We have used magnetic tweezers to characterize Mycobacterium smegmatis topoisomerase I (MsmTOP1). In addition to a conserved core domain common to all type IA topoisomerases, Mycobacteria type IA topoisom erases have unique DNA-binding C-terminal domains that are involved in passage of the second DNA strand through a protein-mediated DNA gate. In separate magnetic tweezers assays, we measured the supercoil relaxation activity and DNA-gate dynamics of the wildtype and mutants lacking portions of the C-terminus. Our results provide new information on the role of these C-terminal domains in the topoisomerase activity. Snack Break 1:45 pm - 3:00 pm, Exhibit Hall BC Poster Presentations and Late Posters 1:45 pm - 3:45 pm, Exhibit Hall BC Teaching Science Like We Do Science 2:00 pm - 4:00 pm, Room 1AB This interactive, hands-on workshop focuses on practice-applicable, easy-to-use strategies and tools that educators at any level of biophysical science education can use to assess what their students take away from their teaching, and where changes to their educational methods might be appropriate. Career Development Center Workshop I Need a Job Right Now – What Do I Do? 2:30 pm - 3:30 pm, Room 16B This session will give a step-by-step strategy to land a job right now, and is applicable to academia, industry, and any other ecosystem. All About ARPA-H Exploring Opportunities at the New Agency 2:30 pm - 4:00 pm, Room 8 The Advanced Research Projects Agency for Health (ARPA-H) will be entering its first full year of operation in 2023. Join the Public Affairs Committee as we learn more about the fledgling high-risk, high-reward research agency, how the structure will continue to form, exploring what opportunities exist and how does that fit into the larger research funding sources available to biophysicists. Exhibitor Presentation Oxford Instruments 2:30 pm - 4:00 pm, Room 10 Multi-Modal Microscopy: Challenges and Workflows for Correlative Microscopy Acquisition and Analysis Correlative microscopy data is beautiful and incredibly complex, particu larly when dealing with data acquired at significantly different magnifica tion scales, 3D data, data acquired using analytical techniques or dynamic data that has been acquired at multiple time points with more than one type of imaging technique. Combining multiple microscopy techniques can have more than one purpose, enabling researchers to better target

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