Biophysical Society 67th Annual Meeting Program Guide

345-pos Board B166 A SWI4 POSITIVE FEEDBACK LOOP THAT REGULATES TIMING OF START REVEALED BY TWO PHOTON SCANNING NUMBER AND BRIGHTNESS MICROSCOPY. Abhishek Ghimire , Carleton H. Coffin, Pooja Goswami, Jing Cheng, Ghada Ghazal, Sylvain Tollis, Mike Tyers, Catherine A. Royer 346-pos Board B167 SINGLE-MOLECULE STUDIES ON CO-TRANSCRIPTIONAL R-LOOP FORMA TION AT DNA DAMAGE SITE. Gun Hyoung Lim , Sungchul Hohng 347-pos Board B168 CYTOKINE-INDUCED TRANSCRIPTIONAL MEMORY IS EVIDENT IN THE KINETICS OF TRANSCRIPTIONAL BURSTS. Clayton Seitz , Hailan Ma, Hok Wai Chang, Jing Liu 348-pos Board B169 PAK4 KINASE AS A POTENTIAL THERAPEUTIC TARGET OF THE TRANSCRIP TION FACTOR P63-MEDITATED SQUAMOUS CELL CARCINOMAS OF THE PANCREAS. Seungeun Lee 349-pos Board B170 DECIPHERING THE MOLECULAR MECHANISMS OF BPTF INTERACTIONS WITH NUCLEOSOMES VIA MOLECULAR SIMULATIONS. Ryan F. Hebert , Jeffery M. Wereszczynski 350-pos Board B171 THE A12.2 SUBUNIT HAS A ROLE IN RNA POLYMERASE I PYROPHOSPHATE RELEASE. Kaila B. Fuller , Ruth Q. Jacobs, David A. Schneider, Aaron L. Lucius 351-pos Board B172 RECONSTITUTION OF MITOCHONDRIAL TRANSCRIPTIONAL PAUSING. An Board B173 INVESTIGATING THE MOLECULAR MECHANISMS BEHIND THE TRAN SCRIPTIONAL RESPONSE IN PRESSURE ADAPTATION. Carleton H. Coffin , Brooke Evans, Luke Fisher, Abhishek Ghimire, Doug Bartlett, Catherine A. Royer Protein-Nucleic Acid Interactions I (Boards B174 - B205) 353-pos Board B174 SINGLE-MOLECULE DISSECTION OF THE INTERACTION BETWEEN A METHYL-DNA BINDING PROTEIN AND CHROMATIN. Gabriella N.L. Chua 354-pos Board B175 STRUCTURE AND FUNCTION OF SARS-COV-2 NUCLEOCAPSID PROTEIN MEASURED USING OPTICAL TWEEZERS, CONFOCAL FLUORESCENCE, AND AFM. Michael Morse , Jana Sefcikova, Micah J. McCauley, Ioulia F. Rouzina, Penny J. Beuning, Mark C. Williams 355-pos Board B176 SINGLE MOLECULE EVIDEONCE FOR LINKER HISTONE H1 DNA SLIDING AND NUCLEOSOMAL BYPASSING REGULATED BY THE C-TERMINAL DISOR DERED DOMAIN. Ehsan Akbari , Nathan Burge, Michael G. Poirier 356-pos Board B177 POLARITY OF THE CRISPR ROADBLOCK TO TRANSCRIPTION. Porter M. Hall , James Inman, Robert M. Fulbright, Tung T. Le, Joshua Brewer, Guil laume Lambert, Seth A. Darst, Michelle D. Wang 357-pos Board B178 MIRNA ACCESSIBILITY IN CELLULAR RISC REVEALED BY SINGLE-MOLECULE KINETIC FINGERPRINTING. Tanmay O. Chatterjee , Shankar Mandal, Nils G. Walter 358-pos Board B179 STUDYING PICH-INDUCED NUCLEOSOME REMODELING USING DUAL TRAP OPTICAL TWEEZERS. Dian Spakman, Tinka V.M. Clement , Andreas S. Biebricher, Graeme A. King, Manika I. Singh, Ian D. Hickson, Erwin J.G. Peterman, Gijs J.L. wuite H. Hsieh 352-pos

359-pos Board B180 DNA MINI-CIRCLES REVEAL EFFECTS OF DNA TOPOLOGY ON CRISPR-CAS9 FUNCTIONS. Yukang Liu , Peter Z. Qin 360-pos Board B181 THE SMC5/6 COMPLEX EXHIBITS DYNAMIC BINDING ON DOUBLE STRANDED DNA, BUT BINDS STABLY TO BRANCHED DNA. Jeremy T. Chang , Shibai Li, Emily Beckwitt, Thane Than, Cory Haluska, Joshua Chan danani, Michael O’Donnell, Xiaolan Zhao, Shixin Liu 361-pos Board B182 EXPLORING CLEAVAGE ACTIVITY OF NSP15 USING SINGLE-MOLECULE PIE-FRET. Kenya Gordon , Meredith Frazier, Isha Wilson, Robin Stanley, Sharonda LeBlanc 362-pos Board B183 PROBING THE BRIDGING BEHAVIOUR OF THE ROK PROTEIN FROM BACIL LUS SUBTILIS WITH QUADRUPLE-TRAP OPTICAL TWEEZERS. Xamanie M.R. Seymonson , Panagiotis Christodoulis, Mandy Erkelens, Andreas S. Biebricher, Remus T. Dame, Gijs J. Wuite 363-pos Board B184 TARGET SEARCH DYNAMICS OF SOX TRANSCRIPTION FACTORS. Sim Sakong , David Suter, Beat Fierz 364-pos Board B185 OPTICAL TWEEZERS EXPERIMENTS REVEAL ANTICANCER DRUG BI-3802 BINDS DNA IN THE MICROMOLAR RANGE. Jared M. McAuliffe , Mark C. Williams, Thayaparan Paramanathan 365-pos Board B186 Travel Award Winner IMPACT OF SHELTERIN COMPLEX ON TELOMERE ACCESSIBILITY. Sajad A. Shiekh , Amanda Jack, GolamMustafa, Sineth G. Kodikara, Prabesh Gyawali, Mohammed E. Hoque, Ahmet Yildiz, Hamza Balci 366-pos Board B187 HIV-1 NUCLEOCAPSID PROTEIN BINDS DOUBLE-STRANDED DNA IN MUL TIPLE MODES TO REGULATE COMPACTION AND CAPSID UNCOATING. Helena Gien , Michael Morse, Micah J. McCauley, Ioulia F. Rouzina, Jona than Kitzrow, Karin Musier-Forsyth, Robert Gorelick, Mark C. Williams 367-pos Board B188 T4 GP32 FORMS DYNAMIC, COMPACTING FILAMENTS ON SSDNA THAT REORGANIZE THROUGH INTERPROTEIN INTERACTIONS. Benjamin Cash en , Michael Morse, Ioulia F. Rouzina, Richard L. Karpel, Mark C. Williams 368-pos Board B189 MEASURING SITE SPECIFIC DNA CLEAVAGE AT THE SINGLE-MOLECULE LEVEL. Allen C. Price, Jaqueline Ferreira , Lauren Menzel, Krysten Rafferty 369-pos Board B190 “HELICASE” ACTIVITY PROMOTED THROUGH DYNAMIC INTERACTIONS BETWEEN A SSDNA TRANSLOCASE AND A DIFFUSING SSB PROTEIN. Kacey N. Mersch , Joshua E. Sokoloski, Binh Nguyen, Roberto Galletto, Timothy M. Lohman 370-pos Board B191 MEASURING DNA FOLDING BY THE SARS-COV-2 NUCLEOCAPSID PRO TEIN. Jake J. Kim , Youna N. Choi, Ashley R. Carter 371-pos Board B192 A KINETIC MECHANISM FOR U6 SNRNP BIOGENESIS OBTAINED BY SINGLE-MOLECULE FLUORESCENCE COLOCALIZATION MICROSCOPY. Ye Liu , Yuichiro Nomura, Samuel E. Butcher, Aaron A. Hoskins 372-pos Board B193 SINGLE-MOLECULE ASSAY FOR HIGHLY SENSITIVE SARS-COV-2 GENE DETECTION BASED ON CRISPR-CAS12A. Sangmin Ji , Hye Ran Koh

F R I D A Y

S U N D A Y

34