Biophysical Society 67th Annual Meeting Program Guide

Exhibits 10:00 am - 5:00 pm, Exhibit Hall BC Meet the Editors, The Biophysicist 10:15 am - 11:00 am, Lobby BC/Publications Booth

Dr. Jean-Francois Rolland will provide an overview of when and how automated patch clamp instruments are used for supporting ion chan nel drug discovery programs (cell line generation, primary screening, hit-to-lead/SAR, compound profiling and / or “mode of actions” studies) in Axxam. Dr. Rolland’s presentation will also include a comparison of data generated using surrogate techniques and with the manual patch clamp. In particular, he will describe experiments where the intracellular solution perfusion, an extremely difficult technique to perform using conventional patch clamp, was used with ease using the Port-a-Patch and SyncroPatch 384. Additionally, Dr. Rolland will show experiments where ligand-gated ion channels were studied using the ‘Ligand Puff’ feature of the SyncroPatch 384 (fast external solution addition and low exposure times) and finally, give a glimpse of how automated patch-clamp can be associated with optogenetics. Dr. James Costantin will focus on activation and pharmacology nicotinic acetylcholine receptors. Nicotinic acetylcholine receptors (nAChR) are cation-permeable ion channels, which mediate fast synaptic transmission when activated by the endogenous neurotransmitter acetylcholine (ACh) or the exogenous natural alkaloid, nicotine. nAChα7R are widely distrib uted in the mammalian brain including in the cerebral cortex, hippocam pus, basal ganglia and cerebellum and have been proposed to play a role in many neurological disorders such as Alzheimer’s Disease, Parkinson’s, schizophrenia and depression. The most abundantly expressed nAChR in the mammalian brain are the α7 homomeric and α4β2 heteromeric receptors. Nicotinic α7 are particularly challenging to record using patch clamp because of their extremely fast desensitization. Dr. Costantin will show activation and pharmacology of α4β2 and α7/ric3 receptors using the well-based high throughput automated patch clamp system, the SyncroPatch 384. Transporters and ion pumps are transmembrane proteins that facili tate ion flux across biological membranes against their concentration gradient. Solid supported membrane (SSM) electrophysiology using the SURFE2R instruments from Nanion can be used to measure electrogenic membrane transport, even with low turnover rates, as well as ligand gated, and leak channels. Any membrane source can be used, meaning that intracellular transporter and channels still present in organellar membrane can be targeted using this method, allowing for direct and functional recordings in a physiologically relevant context. Transporters and intracellular ion channels are increasingly important pharmacological targets for the treatment of a wide variety of diseases. In this presenta tion, scientist Nataliia Dmitrieva, will present her work on the molecular basis of the function of DgoT, a bacterial homolog of vesicular glutamate transporters. Speakers Andrea Brüggemann, CSO, Nanion Technologies James Costantin, Scientific Market Development Manager – Ion Chan nels, Eurofins DiscoverX Nataliia Dmitrieva, Forschungszentrum Jülich Jean-Francois Rolland, Head, Electrophysiology, Deputy Director, Discov ery Services Axxam SpA

M O N D A Y

Take this opportunity to meet editors of The Biophysicist . Editors will be available to talk about the journal and answer questions about where you should submit your work, what will help to get your work published, and what you can expect when you submit. Editor-in-Chief Sam Safran and other editors will be present at the Biophysical Society publications booth. Coffee Break

10:15 am - 11:00 am, Exhibit Hall BC New Member Welcome Coffee 10:15 am - 11:15 am, Room 1AB

Calling all new BPS members! Come and mingle with BPS Staff, Soci ety Council, and programmembers as you learn about the Society’s activities. Current members are welcome to attend and meet with new members. Career Development Center Workshop Marketing Your Value: Crafting your Elevator Pitch/30 Second Value Statement/Brand Statement 10:30 am - 11:30 am, Room 16B In this workshop, we will address how to effectively market yourself for career opportunities (academia, industry and beyond) and leverage and utilize marketing methods and channels, and we will spend time on how to craft an elevator pitch and what makes a great pitch for employers. Exhibitor Presentation Bruker 10:30 am - 12:00 pm, Room 10 EPR Research Solutions from Routine Analytics to High-End Experimen tation Speaker Ralph Weber, Senior Applications Scientist, Bruker BioSpin Corporation Bruker EPR presents new hardware and software advances to answer your research needs. NMR Research Solutions for Biophysical Applications Speaker Clemens Anklin, VP of NMR Applications, Bruker BioSpin Corporation After the successful introduction of 1.2 GHz magnets, Bruker BioSpin has been able to apply the HTS technology to create a 1.0 GHz system with a significantly smaller footprint. The Ascend EVO 1 GHz magnet operates at 4.2 K, using 1.3 of the amount of helium of previous 1GHz magnets. Due to its reduced height and smaller stray field, it can easily be sited in a single story lab. Other new developments include probes, acquisition, processing, and analysis software.

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