Biophysical Society 67th Annual Meeting Program Guide

fitting methods, such as phasor FLIM (2) and minimal fraction of donor molecules (mfD) approach (3). All these approaches are available in the STELLARIS confocal platform. In particular, mfD has been implemented as a TauSense tool named TauInteraction. The advanced tools of STELLARIS confocal platform are also available un der cryogenic conditions, enabling advanced cryo correlative (cryo-CLEM) studies. Here, new cellular mechanisms can be unraveled. Intracellular protein interactions can be further resolved by cryo electron microscopy (EM) down to the subnanometer scale. To increase the success rate of these types of studies, Cryo light microscopy enables the identifica tion of specific target proteins prior to the EM imaging. LIGHTNING and TauSense provide high accuracy in identifying and targeting the desired proteins for further analysis. Speaker Giulia Ossato, Senior Product Manager, Leica Microsystems Envisioning the Future of Biophysical Postdoctoral researchers represent a major portion of the academic research work force. While the position was originally intended as a few years of post-graduate training prior to becoming an independent investi gator, it has since evolved into an often long-term position with uncertain outcomes. Not only has this been detrimental to postdocs themselves, but this has led to the development of a host of other systemic issues throughout academic research. Join the Public Affairs Committee as we host a discussion to about the future of a keystone position in biophysics research. Moderator Michael Rudokas, Yale University Speakers Dorothy Beckett, NIGMS Melanie Cocco, University of California, Irvine Devasena Ponnalagu, Ohio State University Shanna Hamilton, Ohio State University Biophysics 101: Incorporating Bayesian and Artificial Intelligence Approaches into Biophysics 1:30 pm - 3:00 pm, Room 6E Biophysics 101 is an opportunity to learn about hot topics in Biophysics from experts in the field! This year, speakers will talk about incorporating Bayesian and Artificial Intelligence approaches into Biophysics. Speakers Pilar Cossio, Flatiron Institute, USA Pratyush Tiwary, University of Maryland at College Park, USA Snack Break 1:45 pm - 3:00 pm, Exhibit Hall BC Poster Presentations and Late Posters 1:45 pm - 3:45 pm, Exhibit Hall BC Postdoctoral Training 1:00 pm - 2:30 pm, Room 7AB

multiplexed probe molecules arrayed on the sensor surface. However, accurate characterization of large analytes (50-150nm size) presents challenges due to non-linearity of the optical interference signal with surface binding of sparse BNP analytes. We have recently demonstrated that the affinity of large analytes (extracellular vesicles) can be character ized, qualitatively, on IRIS chips benefiting from IRIS’ capability of uniform illumination at different wavelengths. In this presentation, we will discuss how the improved IRIS platform can bridge the gap between single-particle detection (’digital’ configuration) and ensemble reflectance measurements (’analog’ configuration), creat ing a new ’hybrid’ system (h-IRIS), providing a substantial improvement in sensitivity, improving the limit of detection by nearly three orders of magnitude. This new hybrid configuration of IRIS combines the advanta geous features of high-magnification and low-magnifications modali ties. Unique capability of the IRIS platform to conduct kinetic binding measurements of individual analytes has been also demonstrated for antibody characterization and a novel competition assay for therapeutic antibodies for viral infections. We believe that the scientific community will greatly benefit from our approach of combining SP-IRIS platform and use of whole virus particles for obtaining competition information for monoclonal antibodies. Speaker Selim Unlu, Distinguished Professor, Boston University Career Development Center Workshop Translating Your Credentials: Writing Effective resumes + Cover Letters for Industry, Govern ment, and Nonprofit Jobs 12:00 pm - 1:00 pm, Room 16B You have a CV with its list of publications, presentations, honors & awards, etc., but jobs outside of academia typically require a resume. Very few job ads fail to mention the importance of teamwork, collabora tion, and communication skills, yet if you take a glance at your CV, these are all likely to be absent or incomplete. Learn how to transform your CV into an effective resume that helps hiring managers recognize and ap preciate the value you have to offer. Exhibitor Presentation Leica Microsystems 12:30 pm - 2:00 pm, Room 10 Molecular Interaction Across Imaging Modalities with the Leica STEL LARIS Confocal Platform Molecular interactions and dynamics are the foundation of cellular ma chinery. For molecules to interact, they must come close enough in space so they can exert a chemical influence on their molecular environment. Confocal fluorescence imaging became a pillar in such endeavors for the specificity conferred by fluorochromes, and the spatial context, spatial and temporal resolution for the inherent dynamic nature of the interac tion process. The ultimate tool of choice to study the interaction of two molecules is Förster Resonant Energy Transfer (FRET), where the molecules of interest are tagged with probes that act as a “donor-acceptor pair” (1). Many FRET approaches rely on intensity-based measurements such as quenching of donor, acceptor photobleaching, and sensitized emission. Intensity-based FRET requires basic instrumentation but has high risks of artifacts from experimental design. The gold standard to measure FRET is FLIM (fluorescence lifetime imag ing microscopy), as the FRET readout here depends only on the donor fluorescence lifetime changes. The analysis of FLIM-FRET can be done by fitting the fluorescence decay with a multi-exponential model or non

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