Biophysical Society 67th Annual Meeting Program Guide

Authorship Best Practices in Biophysics 2:15 pm - 3:45 pm, Room 8 This panel, organized by the BPS Publications Committee, will feature a panel of biophysicists to share their experiences and answer your ques tions about how authorship is determined for research papers in biophys ics. Panelists will represent scientists from diverse backgrounds in differ ent types of academic departments. They will discuss their approaches to determining first and corresponding author, navigating disagreements about authorship, and other related topics. Moderator Carlos Baiz, University of Texas at Austin, USA Panelists Antje Almeida, University of North Carolina Wilmington, USA Kandice Tanner, NIH, USA Sarah Veatch, University of Michigan, USA Career Development Center Workshop The Industry Interview: What You Need to Do Before, During, and After to Get the Job 2:30 pm - 3:30 pm, Room 16B In this session we will focus on learning and using the language of industry and referencing the culture and priorities of industry to ace the interview. We will discuss how to effectively market yourself in these interviews and clarify how your skills and experience are an asset to a company. Specifically, we will address this for pharma and biotech inter views. We will run through some of the most common questions asked in industrial interviews and give specific advice about how to set yourself apart. Achieving Work-Life Balance Across Different Job Sectors 2:30 pm - 4:00 pm, Room 2 Whether you are in academia thinking about how much more time you would have for family if you worked in the biotechnology sector, or you work in government wishing for more control over your calendar, most people want better work-life balance. It can be tempting to see only the advantages of careers different from yours. In this panel discussion, we will hear from biophysicists working in academia, industry, and the US government about the challenges of achieving work-life balance across different work sectors, and the benefits of each. Exhibitor Presentation Nanosurf 2:30 pm - 4:00 pm, Room 10 Application of Low-Force Photothermal Off-Resonance Tapping For In Situ AFM Imaging of Soft Virus Capsids The atomic force microscope (AFM) is becoming an essential tool to image and measure the mechanical properties of biological samples in near-physiological conditions. Unlike optical or electron microscopy tech niques, samples measured by AFM require very minimal preparation. The primary feedback mechanism in AFM is the force between the sample being measured and a probe. When working with soft biological samples, the force generated by the probe can often be enough to cause irrevers ible damage, or in some cases, physically remove the sample from the substrate if lateral stresses become too high. For this reason, imaging soft biological materials such as virus capsids on top of another soft substrate material such as cells or tissue continues to be a challenge for the AFM community. One of the most popular dynamic force AFM imaging techniques (some times called amplitude modulation or tapping mode) works by trying to maintain the amplitude of an oscillating probe near is resonance fre

quency as it traces the contours of a surface. While it is simple enough to measure changes in amplitude, using this to determine how much force was applied to the surface requires complex technical modeling, which is oftentimes beyond the interest of general microscopists. More often, it takes a skilled AFM operator to instinctively know what settings to use for any specific sample. Developing this level of expertise has a steep learn ing curve and it is often frustrating for those new to the technique. Photothermal off-resonance tapping mode (e.g. WaveMode) is a tech nique that combines the force selectivity of contact mode while at the same time minimizing sheer or lateral forces, which can displace loosely bound or soft materials. The benefits of a photothermally actuated canti lever for imaging at or near the resonance of the cantilever for biological applications has been well documented. Using the same photothermal light source to drive the cantilever off-resonance is a more novel tech nique. First, since the measurement is done off-resonance, the Q-factor of the cantilever is irrelevant, and thus the imaging bandwidth increases. Second, because the feedback mechanism is force-deflection via ampli tude truncation and not peak-to-peak amplitude reduction, the force that is applied to the sample can be precisely controlled down to the low pico-Newton range. Third, off-resonance tapping mode is still considered an intermittent contact mode meaning that the probe only interacts with the surface for only a small portion of its oscillation, thus minimizing lateral stress while the probe is scanned across the surface. Recently, WaveMode was used to image HSV-1 virus capsids on a soft rat-liver nuclei substrate. One of the challenges for imaging this type of sample with AFM is that the capsid is expected to be stiffer than the sur rounding substrate, which could cause the capsid to be pushed into the nuclei. It has been shown that the nucleus exhibits a viscoelastic stiffness response, which increases its stiffness as the AFM indentation velocity increases. Thus, imaging in WaveMode causes the nucleus to stabilize around the capsid and allows for clean images of the complex. Speaker Eugene Ogorodnik, Technical Sales and Applications, Nanosurf JUST-B (Justice for Underrepresented Scholars Training in Biophysics) Poster Session 3:00 pm - 5:00 pm, Ballroom Lobby The JUST-B (Justice for Underrepresented Scholars Training in Biophysics) Poster Session will celebrate the achievements of underrepresented and underserved students, postdocs, and early career researchers in the field of biophysics. The poster session will promote the endeavors of trainees, offer support through networking opportunities, and serve as a recruit ment venue for those seeking to diversify their pool of future graduate students, postdocs, faculty, staff scientists, journal editors, or any other career within biophysics. All are welcome to attend. Exhibitor Presentation A-TEEMMolecular Fingerprinting For Biopharmaceutical Products Such As Vaccines, Insulin, Viral Vectors (AAVs), Cell Media, Exosomes, and More A-TEEM is a novel, robust, and extremely sensitive analytical technique comprising the best attributes of molecular spectroscopy (speed, low per measurement cost, lab-to-line placement) and chromatography (sensitiv ity, selectivity, low limits of detection and quantification). The A-TEEM technique combines absorbance and fluorescence spectros copy in one simultaneous measurement, providing five characteristics that together create a unique A-TEEM fingerprint: The Extinction Coef ficient and Absorbance Spectrum (from absorbance) as well as Quantum Efficiency and the entire Excitation and Emission Spectrum in the form of 3D EEM from fluorescence. The absorbance information is also used to HORIBA Scientific 3:30 pm - 5:00 pm, Room 9

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