Biophysical Society 67th Annual Meeting Program Guide

Symposium Artificial Enzymes: Protein Dynamics and Directed Evolution 4:00 pm - 6:00 pm, Room 6B

correct the fluorescence EEM for inner filter effects, thus facilitating truly quantitative and highly selectable molecular fingerprints. A-TEEM fingerprints of very similar components are often unique, enabling quantitative analysis of responsive components to ppb levels. This makes it a natural fit for the characterization of many relevant bio pharmaceutical products such as Vaccines, Insulin, Viral Vectors (AAVs), Antibody-Drug Conjugates (ADCs), Cell Media, Exosomes, CRISPr/CAS9, and more. As a spectroscopic tool, A-TEEM can be deployed for process analytics, used to monitor bioprocesses, or screen for batch-to-batch variance across a variety of relevant biomolecules. Putting a sample in a cuvette is often the only preparation needed for measurement. For some quantitative measurements, sample dilution may be required. Speaker Linda Kidder Yarlott, Life Sciences Business Development Manager, HORIBA Scientific Membership Committee Meeting 3:30 pm - 5:30 pm, Room 14A Career Development Center Workshop Negotiation for Biophysicists: Negotiation Strategies and Tactics and Evaluating a Job Offer 4:00 pm - 5:00 pm, Room 16B We will address strategies and tactics to evaluate a job offer and to nego tiate for what you are worth in the employment marketplace, including postdoc and faculty roles. Symposium Bacterial Barrier s 4:00 pm - 6:00 pm, Room 6A Chair Randy Stockbridge, University of Michigan, USA 819-symp 4:00 pm BACTERIAL BRAINS: HOW BACTERIA USE ELECTROCHEMICAL POTENTIALS TO SOLVE COMPLEX PROBLEMS. Gurol Suel 820-symp 4:30 pm GENERAL FEATURES OF TRANSMEMBRANE BETA BARRELS FROM A LARGE DATABASE. Joanna Slusky , Daniel Montezano, Rebecca Bernstein, Matthew Copeland 821-symp 5:00 pm MECHANISTIC BASIS OF CHOLINE IMPORT AND LIPID FLIPPING INVOLVED IN TEICHOIC ACID SYNTHESIS AND MODIFICATION. Camilo Perez 822-symp 5:30 pm THE MOLECULAR BASIS FOR ANTISEPTIC RESISTANCE IN BACTERIA. Randy Stockbridge

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Chair Shina Caroline Lynn Kamerlin, Uppsala University, Sweden 712-symp 4:00 pm DIRECTED EVOLUTION OF ARTIFICIAL ENZYMES WITH NEW-TO-NATURE FUNCTIONS. Huimin Zhao 713-symp 4:30 pm EVOLUTION OF ENERGY LANDSCAPES IN DESIGNER ENZYMES. Donald Hilvert 714-symp 5:00 pm WHAT DIRECTED EVOLUTION AND PROTEIN DYNAMICS CAN TEACH US ABOUT ARTIFICIAL ENZYME DESIGN. Steven D. Schwartz 715-symp 5:30 pm LOOP DYNAMICS AND EVOLVABILITY IN THE TIM-BARREL PROTEINS OF HISTIDINE AND TRYPTOPHAN BIOSYNTHESIS. Adrian Romero-Rivera, Marina Corbella Morató, Antonietta Parracino, Wayne M. Patrick, Shina Caroline Lynn Kamerlin Platform Smooth and Cardiac Muscle I 4:00 pm - 6:00 pm, Room 6C Co-Chairs 4:00 pm CALCIUM AND MYOSIN S1 BINDING CAUSE STRUCTURAL REARRANGE MENT OF THE C-TERMINUS OF HUMAN CARDIAC TROPONIN T. Garrett Crosby , Jil C. Tardiff 824-plat 4:15 pm DEFINING THE STRUCTURE AND DYNAMICS OF THE N-CTNI- N-CTNC INTERFACE IN CARDIAC THIN FILAMENTS. Romi Castillo 825-plat 4:30 pm MODELING THE TROPONIN CORE DOMAIN ON THIN FILAMENTS USING DATA FROM CRYOELECTRON MICROSCOPY AND FLUORESCENCE AP PROACHES. Michael J. Rynkiewicz , Elumalai Pavadai, Ivanka Sevrieva, Malcolm Irving, William Lehman 826-plat 4:45 pm USING 2’-DEOXY-ADP TO PROBE STABILITY OF THE MYOSIN INTERACTING HEADS MOTIF AT ATOMIC RESOLUTION. Matthew C. Childers , Michael Regnier 827-plat 5:00 pm MECHANISMS OF CARDIAC MYOSIN REPLACEMENT. Colleen M. Kelly , Jody L. Martin, Molly Coseno, Michael J. Previs 828-plat 5:15 pm PHOSPHOLAMBAN INHIBITS THE CARDIAC CALCIUM PUMP BY REVERS ING ALLOSTERIC EFFECTS OF ATP ON CALCIUM AFFINITY. Sean R. Cleary , Jaroslava Seflova, Ellen E. Cho, L. Michel Espinoza-Fonseca, Seth L. Robia 829-plat 5:30 pm MYOSIN-BINDING PROTEIN C (MYBP-C) STABILIZES, BUT IS NOT THE SOLE DETERMINANT OF SRX MYOSIN IN CARDIAC MUSCLE. Shane R. Nelson , Samantha B. Previs, Sakthivel Sadayappan, Carl W.C. Tong, David M. Warshaw Sean Cleary, Loyola University Chicago, USA Garrett Crosby, University of Arizona, USA 823-plat

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