Biophysical Society Thematic Meeting | Singapore

Mechanobiology of Disease

Poster Abstracts

78-POS Board 78 Curvature Effect on Monolayer Cell Migration Wang Xi 1,2 , Surabhi Sonam 2,3 , Benoit Ladoux 2,4 , Chwee Teck Lim 1,2,5 ,

1 Centre for Advanced 2D Materials, Singapore, Singapore, 2 Mechanobiology Institute, Singapore, Singapore, 3 Department of Biomedical Engineering and Department of Mechanical Engineering, Singapore, Singapore, 4 Institut Jacques Monod, Paris, France, 5 Singapore MIT Alliance Research and Technology, Singapore, Singapore. In vivo cell sheets constitute a variety of curved architectures such as lung alveoli, kidney nephron and breast acini. Notably, during certain organ development and wound healing processes, cell monolayers often advanced on a curved surface. While a considerable amount of effort has been made to study monolayer cell migration, most of the studies have primarily used 2D-cell culture systems, which fail to recapitulate the complex geometric nature of tissues and organs. The mechanistic basis of cell behavior on a 3D curvature also remains poorly understood. Here, we investigate monolayer cell migration on various curved surfaces. We study intra-cellular arrangements, cell polarity and cell sheet velocity. Our findings reveal interesting insights into migration of cell monolayer in response to extent of curvature and can assist in better understanding tissue morphogenesis.

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