Program Book

120-P lat 9:15 am SINGLE-MOLECULE TRACKING OF SMOOTHENED IN THE PRIMARY CILIUM. Lucien E. Weiss , Ljiljana Milenkovic, Steffen J. Sahl, Theodore L. Roth, Matthew P. Scott, W E. Moerner 121-P lat 9:30 am DIFFERENTIAL CLUSTERING OF SRC FAMILY KINASE ON LIPID BILAYERS REGULATES A NET PHOSPHORYLATION ACTIVITY IN A RECEPTOR-KINASE-PHOSPHATASE NETWORK. Gabriela Furlan, Li Huang, Takashi Minowa, Nobutaka Hanagata, Chiho Kataoka, Yoshihisa Kaizuka 122-P lat 9:45 am OPTICAL PROBING OF METABOTROPIC GLUTAMATE RECEPTOR ASSEMBLY AND COOPERATIVITY. Josh Levitz , Shashank Bharill, Reza Vafabakhsh, Ehud Y. Isacoff 123-P lat 10:00 am THE STRUCTURE AND OLIGOMERICITY OF THE TRANSMEMBRANE DOMAIN OF CYTOKINE RECEPTORS IS MODULATED BY THE PROTEIN/LIPID RATIO. Katrine Bugge , Kresten Lindorff-Larsen, Michael J. Waters, Vincent Goffin, Birthe B. Kragelund Co-Chairs Marco Capitanio, University of Florence, Italy Timothy Blosser, Delft University of Technology, The Netherlands 124-P lat 8:15 am PROTEIN DNA INTERACTION MEASURED WITH ULTRA FAST FORCE CLAMP OPTICAL TWEEZERS. Carina Monico, Alessia Tempestini, Francesco Vanzi, Francesco Pavone, Marco Capitanio 125-P lat 8:30 am MECHANICAL CHARACTERIZATION OF A FAST DNA MOTOR: SPOIIIE. Ninning Liu , Gheorghe Chistol, Carlos Bustamante 126-P lat 8:45 am international travel awardee STUDYING PROTEIN-DNA DYNAMICS AND PROTEIN UNFOLDING USING A FORCE-FREE SINGLE-MOLECULE TECHNIQUE. Guy Nir , Moshe Lindner, Yuval Garini 127-P lat 9:00 am MULTIPLE LAC-MEDIATED LOOPS REVEALED BY BAYESIAN STATISTICS ANDTETHERED PARTICLE MOTION. Martin Lindén , Stephanie Johnson, Jan-Willem van de Meent, Rob Phillips, Chris Wiggins 128-P lat 9:15 am SUBSTRATE RECOGNITION AND SPECIFICITY OF DOUBLE- STRANDED RNA BINDING PROTEINS. Lela Vukovic , Hye Ran Koh, Sua Myong, Klaus Schulten 129-P lat 9:30 am MOLECULAR MECHANISM OF INHIBITION OF THE PKR-RNA INTERACTION BY THE INFLUENZA A VIRUS NS1 PROTEIN– A THREE COLOUR BASED FLIM-FRET APPROACH IN LIVING CELLS. Fabian Jolmes , Sieben Christian, Thorsten Wolff, Andreas Herrmann 130-P lat 9:45 am ELUCIDATING RESTRICTION ENDONUCLEASES REACTION MECHANISMS VIA DWELL-TIME DISTRIBUTION ANALYSIS. Candice M. Etson , Petar Todorov, David R. Walt 8:15 am –10:15 am , R oom 306 Platform Protein-Nucleic Acid Interactions I

131-P lat 10:00 am DEFENSE AGAINST VIRAL ATTACK: SINGLE-MOLECULE VIEW ON A BACTERIAL ADAPTIVE IMMUNE SYSTEM. Timothy Blosser , Edze Westra, Luuk Loeff, Cees Dekker, Stan Brouns, Chirlmin Joo 9:00 am –10:00 am , R oom 300 Career Center Workshop Beyond the Bench: Preparing for Your Career Transition in the Life Sciences There are numerous alternative career options for the seasoned bench scientist who may have decided to take his/her talents and apply them in a new direc- tion. This transition can be accomplished without having to matriculate in another graduate program, and this session explores the how’s and why’s of making such a transition. Be prepared to talk about the role you are thinking about moving into, why you may have chosen this alternative path, and what successes you may have had thus far. 9:00 am –10:30 am , R oom 122 CPOW Committee Meeting Forte Bio, A Division of Pall Life Sciences Developing Assays for Kinetic Characterization on the BLItz System The BLItz system is a simple-to-use, affordable label-free assay system capable of delivering rich insight into real-time binding interactions of antibodies and proteins. This workshop will present several case studies of how the BLItz system is being used to validate biophysical models. As part of the workshop, learn tips and tricks for developing a kinetics assay on the BLItz system and take the opportunity to perform a hands-on demo of the system. Presenter: Renee Tobias, Applications Scientist, Pall ForteBio Visit this 3-D portable Dome, sponsored by the Public Affairs Committee, to see how difficult biophysical topics can be made accessible to the public. Short videos that communicate the excitement of looking at macromo- lecular complexes and understanding the molecular basis for life are being shown throughout the week. 10:00 am –5:00 pm , H all D Exhibits 10:15 am –11:00 am , H all D Coffee Break 10:30 am –11:30 am , R oom 300 Career Center Workshop Career Catalyst: Understand Who You Are to Get What You Want Confucius said: “Learning without thought is labor lost; thought without learning is perilous.” Consider investing some time in this updated self- reflection workshop to learn, think and talk about whom you are and what you want. Truly understanding yourself—your likes/dislikes, your passions, preferences, and personality quirks—can lead not only to career success but to a happy and fulfilling life. This workshop will provide tools and tech- niques to help you translate self-reflection into meaningful career choices. 9:00 am –10:30 am , R oom 123 Exhibitor Presentation 10:00 am –5:00 pm , H all D Biomolecular Discovery Dome

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Biophysical Society 58 th Annual Meeting, San Francisco, California