Program Book

8:15 am –10:15 am , R oom 303 Platform Protein-Lipid Interactions I

109-P lat 8:30 am PROBING DNA METHYLATION IN BREAST CANCER CELL LINES USING SOLID-STATE NANOPORES. Azadeh Bahrami , Eric W. Lam, Tim Albrecht 110-P lat 8:45 am LABEL-FREE DETECTION OF THE P53-DNA COMPLEX. Philippa Nuttall , Eric Lam, Tim Albrecht 111-P lat 9:00 am THERMODYNAMIC CHARACTERIZATION OF PROTEINS WITH ELECTRICALLY ACTUATED DNA NANOLEVERS. ThomasWelte , Ralf Strasser, Frank Fischer, Wolfgang Kaiser, Ulrich Rant 112-P lat 9:15 am EVALUATING INTRACELLULAR CROWDED WITH A GLYCINE-INSERTED MUTANT FLUORESCENT PROTEIN. Takamitsu J. Morikawa , Hiroaki Machiyama, Kazuko Okamoto, Keiko Yoshizawa, Hideaki Fujita, Taro Ichimura, Katsumi Imada, Takaharu Nagai, Toshio Yanagida, Tomonobu M. Watanabe 113-P lat 9:30 am NIR FLUORESCENT PROTEINS WITH SYNTHETIC CHROMOPHORES FOR DEEP TISSUE IMAGING. Ming Zhang 114-P lat 9:45 am GENETICALLY ENCODED GAS NANOSTRUCTURES AS BIOPHYICALLY TUNABLE MOLECULAR REPORTERS FOR MRI AND ULTRASOUND. Mikhail G. Shapiro 115-P lat 10:00 am LABEL-FREE OPTICAL DETECTION AND SUPER-RESOLUTION MICROSCOPY OF SINGLE PROTEINS. Vahid Sandoghdar , Marek Piliarik

F R I D A Y

S A T U R D A Y U N D A Y

Co-Chairs Scott Feller, Wabash College Ka Yee Lee, University of Chicago 100-P lat 8:15 am

REPLICA EXCHANGE UMBRELLA SAMPLING SIMULATIONS PROVIDE INSIGHT INTO THE ROLE OF DOCOSAHEXAENOIC ACID IN MODULATING THE STABILITY OF TRANSMEMBRANE PROTEINS. Ryan Snyder, Bo Wang, Matthew Roark, Scott E. Feller 101-P lat 8:30 am PROTON-COUPLED WATER AND HYDROGEN-BOND DYNAMICS IN CHANNELRHODOPSIN. Christopher Mielack, Coral del Val, Maria Luiza Bondar, Ana Nicoleta Bondar 102-P lat 8:45 am APPLICATION OF THE VIRTUAL SITE TECHNIQUE TO LIPIDS IN GROMACS, HYDROGENS DEGREES OF FREEDOM REMOVAL AND PERFORMANCE INCREASE. Bastien Loubet , Wojciech Kopec, Himanshu Khandelia 103-P lat 9:00 am MOLECULAR DETAILS OF α -SYNUCLEIN MEMBRANE ASSOCIATION REVEALED BY NEUTRON REFLECTOMETRY. Jennifer C. Lee , Zhiping Jiang, Sara Hess, Ryan P. McGlinchey, Thai Leong Yap, Frank Heinrich 104-P lat 9:15 am UNDERSTANDING PROTEIN-MEMBRANE INTERACTIONS VIA FREE ENERGY CALCULATIONS. Joakim P. M. Jämbeck , Alexander P. Lyubartsev 105-P lat 9:30 am A MOLECULAR MECHANISM FOR DIFFERENTIAL RECOGNITION OF MEMBRANE PHOSPHATIDYLSERINE BY THE IMMUNE REGULATORY RECEPTOR TIM4. Gregory T. Tietjen, Zhiliang Gong, Chiu-Hao Chen, Ernesto Vargas, James E. Crooks, Kathleen D. Cao, Charles T.R. Heffern, J. Michael Henderson, Benoit Roux, Mati Meron, Binhua Lin, Mark Schlossman, Theordore L. Steck, Erin J. Adams, Ka Yee C. Lee 106-P lat 9:45 am THE POINT OF NO RETURN; 3D STRUCTURE OF BAX-MEDIATED PORES IN MEMBRANE BILAYERS. Dorit Hanein , Xiao-Ping Xu, Dayong Zhai, Eldar Kim, Mark Swift, John C. Reed, Niels Volkmann 107-P lat 10:00 am VOLTAGE- AND CALCIUM-DEPENDENT TOXIN TRANSLOCATION ACROSS A TETHERED LIPID BILAYER. Joel Chopineau , Remi Veneziano, Claire Rossi, Jean-Marie Devoisselle, Alexandre Chenal, Daniel Ladant

8:15 am –10:15 am , R oom 305 Platform Membrane Receptors and Signal Transduction I

Co-Chairs Monica Mazzolini, SISSA, Italy Geoffrey O’Donoghue, University of California, Berkeley 116-P lat 8:15 am

DIRECT, SINGLE MOLECULE, CELL-BY-CELL OBSERVATION OF MOLECULAR KINETICS AND THERMODYNAMICS IN EARLY LYMPHOCYTE SIGNALING. Geoffrey P. O’Donoghue , Rafal Pielak, Jenny J. Lin, Jay T. Groves 117-P lat 8:30 am RESTRICTED SPOTS OF LIGHT REVEAL AN EFFICACY GRADIENT OF THE PHOTOTRANSDUCTION CASCADE ALONG THE ROD OUTER SEGMENT. Monica Mazzolini , Laura Andolfi, Giuseppe Facchetti, Marco Lazzarino, Remo Proietti Zaccaria, Salvatore Tuccio, Johannes Treu, Claudio Altafini, Enzo Di Fabrizio, Gert Rapp, Vincent Torre 118-P lat 8:45 am USING MAGNETIC PROBES TO STUDY RECEPTOR CLUSTERING IN LIVE CELLS. Burcu Celikkol , Alessandra Cambi, Carl G. Figdor, Vinod Subramaniam, Johannes S. Kanger 119-P lat 9:00 am TOWARD SINGLE-MOLECULE IMAGING OF ELECTROPORATED BACTERIAL FLAGELLAR MOTOR PROTEINS IN MOTILE E. COLI. Diana Di Paolo

8:15 am –10:15 am , R oom 304 Platform Biosensors

Co-Chairs Leon Harrington, University of Oxford, United Kingdom Mikhail Shapiro, California Institute of Technology 108-P lat 8:15 am

ELECTROSTATICALLY ENHANCED ASSOCIATION OF A PIM KINASE SUBSTRATE REVEALED BY STOCHASTIC DETECTION. Leon Harrington , Stephen Cheley, Leila T. Alexander, Stefan Knapp, Hagan Bayley

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Biophysical Society 58 th Annual Meeting, San Francisco, California