Program Book

Exhibitor Presentations Room 123 of the Moscone Center

Sunday, February 16 8:00 am–8:45 am FEI Company High End Microscope Platform for Multimodal Live Cell Imaging See page 13 for session description. Presenters Meike Pedersen, Product Marketing Manager, FEI Munich GmbH Tilman Franke, Product Marketing Manager, FEI Munich GmbH Gregor Heiss, Product Marketing Engineer, FEI Munich GmbH 9:00 am–10:30 am Forte Bio, A Division of Pall Life Sciences Developing Assays for Kinetic Characterization on the BLItz System See page 16 for session description. Use-dependent inhibition of ion channels by potential drug candidates is an important aspect to investigate for many drug classes. Use-dependent drugs specifically target ion channels in cells that are more electrically active. For example, a drug targeting pain that is more potent to Na + channels in neu- rons actively firing action potentials is a better drug candidate. Data will be presented to demonstrate the ability of automated electrophysiology systems to study the use-dependence block of Na + channel targets. Tetracaine, lido- caine, and TTX exhibit very different behavior in terms of their use- depen- dent blockage. We will demonstrate the ability of the instrumentation to deliver complex voltage protocols and generate long assay windows which are required for these studies. Pulse trains delivered at 10Hz are used to measure the blockade of current. Data from a separate study will also be presented that demonstrate blockage and enhancement of NaV1.5 currents by various peptide toxins. Both sets of experiments demonstrate stable assay windows with uniform currents for 30 minutes and longer during the delivery of periodic pulse trains. 11:00 am–12:30 pm Molecular Devices, LLC Investigating Use-Dependent Inhibition of Ion Channels on Automated Electrophysiology Systems including the IonWorks Barracuda® Instrument and the IonFlux TM Benchtop Reader

In this presentation, Dr. Johnson will show how global fitting of kinetic data can be accomplished with ease using the fast, dynamic simulation in KinTek Explorer software, overcoming the all-to-common errors in conventional fitting. Moreover, data are fit to derive rate constants directly defining steps in a model. New advances in the software allow fitting kinetic data from single molecule experiments and families of curves can be fit simultaneous to define voltage-dependent rate constants or data from Temperature-jump or Pressure-jump experiments. In addition, equilibrium titration data can be fit using a unique endpoint simulation method, and time-resolved spectra can be fit using singular value decomposition (SVD). Moreover, all experiments can be fit simultaneously. Presenters Kenneth A. Johnson, President, KinTek Corporation Roger Williams, Professor of Biochemistry, University of Texas at Austin 3:00 pm–4:30 pm Nanosurf, Inc. Development of AutomationandNanofluidics toExtendApplications of Atomic Force Microscopy In an effort to extend the range of atomic force microscope (AFM) applications, we have developed automation routines for nanomechanical analysis of large uneven samples and incorporated nanofluidics for nanomanipulation experiments. We will present details of a method that has been developed to com- pensate for the Z-range limitation and to automate the data collection over large sample areas. To compensate for large surface corrugations on biologically relevant samples, customized hardware and software algorithms for automated leveling have been developed and imple- mented. This method consists of a patented vertical alignment system, which is activated whenever the Z piezo reaches its limit (i.e., max. extension or max. retraction). This method allows for AFM investiga- tion to proceed uninterrupted and error-free over corrugated surfaces. FluidFM combines the positional accuracy and force sensitivity of AFM with the unique possibilities of nanofluidics to provide a whole new level of control and possibilities in nanomanipulations and analysis. The FluidFM system includes a fully integrated AFM, pressure control- ler and hollow microfabricated cantilevers. The integrative nature of its touchscreen-based control software brings together optical, force, pressure, and position control in one place. The entire system is easy to use and allows objects and experimental settings to be manipulated via on-screen interactions. Moving a sample or indicating measurement positions has never been more intuitive. Details of several different appli- cations of FluidFM in cell biology will be presented including pick and place of single cells, single cell force spectroscopy, cellular injection and micropatterning under liquids.

Presenter James Costantin, Product Marketing Manager, Automated Electrophysiology, Molecular Devices, LLC

1:00 pm–2:30 pm KinTek 

New Advances in Fitting Kinetic and Equilibrium Data by Simulation Fitting kinetic data based upon numerical integration of rate equations offers many advantages over conventional fitting of data based upon equations derived from simple models. Fitting by simulation is the most rigorous and eliminates the need to derive equations; however, it also requires an under- standing of the kinetics and critical thought to avoid overly complex models.

Presenters Saju Nettikadan, General Manager, Nanosurf, Inc. Brent Lapointe, Research Associate, Biozentrumand the Swiss Nanoscience Institute, Univeristy of Basel

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Biophysical Society 58 th Annual Meeting, San Francisco, California