Program Book

5:00 pm–6:30 pm Asylum Research, an Oxford Instruments Company New blueDrive™ Photothermal Excitation for Superior AFM Tapping Mode Imaging Asylum Research, an Oxford Instruments company, will introduce its new blueDrive Photothermal Excitation capabilities exclusively available on Cypher™, the highest resolution fast scanning AFM. blueDrive sig- nificantly enhances the performance of tapping mode imaging with more simple, stable and quantitative operation, and providing extremely clean tunes in both air and water. Typically, a piezoacoustic excitation has been used to drive the cantilever oscillation. Though piezo drive is favored for design simplicity, the response of the cantilever is often far from ideal, caus- ing users to spend countless time selecting a clean cantilever tune. Asylum’s blueDrive excitation mechanism produces an almost perfect response by directly exciting the cantilever photothermally with a blue laser. blueDrive is ideal for high resolution imaging of biological samples in fluid includ- ing proteins, lipids and nucleic acids, as well as force measurements and nanomechanics. In this presentation, we will explain how blueDrive works, how it achieves simple cantilever tunes, and show real world results for biophysics applications. Presenter Nick Geisse, Applications Scientist, Asylum Research, an Oxford Instruments Company Cryo-TEM: ANewEra for 3DStructural Analysis of ProteinComplexes A new frontier exists in unraveling interactive biological and bio- chemical processes and pathways at the macromolecular level. Of criti- cal importance is the three-dimensional visualization of macromo- lecular structures and molecular machines in their native function- al state. Three techniques play a major role in orchestrating this. Nuclear magnetic resonance (NMR) has the capability to study specific protein domains or fragments and their functional role in protein folding and dynamics and in ligand binding whereas X-Ray crystallography (XRD) allows visualizing high-resolution but more static 3D structures of apo and liganded proteins, mainly in a monomeric or dimeric state after crystallization. To unravel more physiologically relevant situations however, it is essential to visualize multimeric complexes in their tertiary and quaternary state and their interaction with other complexes. By performing typical cryo-TEM applications like single particle analysis or tomography, this can be achieved. In this so-called translational methodology, cryo-TEM thus provides complementary information to NMR and XRD that can be crucial for drug discovery, e.g. in terms of a better understand- ing of the mechanism of action inferred from the EM structure of the physiologically relevant complex. This will eventually contribute to answer real biologically as well as medically relevant questions. Latest developments in the cryo-TEM workflow have brought the three major structural biology technologies closer together. Now, finally, a continuum has been reached on all important aspects with regards to resolution and macromolecular scales which allows for the full deployment of the combination of these technologies. 7:00 pm–8:30 pm FEI Company

Here, we will illustrate the historical context of these technologies with respect to one another and show how latest developments have reached the critical requirements to fully unleash the power of structural biology in not just answering fundamental questions, but actually contribute to curing diseases and improving health. Also, we will discuss the future of structural biology based on the latest developments of the FEI workflow and its components. Presenters Marc Storms, Marketing Manager, Life Sciences, FEI Company Jeff Lengyel, Product Marketing Manager, FEI Company Eric Hnath, Product Marketing Manager, Structural Biology, FEI Company Thomas Wohlfarth, Director, Structural Biology Businesses, FEI Company Monday, February 17 8:00 am–8:45 am FEI Company A Fully Automated Imaging System for Correlative Light and Electron Microscopy See page 66 for session description. Presenters Alex de Marco, Product Marketing Manager, FEI Munich GmbH Gregor Heiss, Product Marketing Engineer, FEI Munich GmbH Liesbeth Hekking, Applications Development Engineer, FEI Company Matthias Langhorst, Segment Director of Cell Biology Solutions, FEI Company 9:00am–10:30am Park Systems, Inc. New Door to Live Single Cell Research Atomic Force Microscopy (AFM) is a powerful measurement technique for nanoscale science. AFM is able to provide high-resolution imaging of biological structures below the optical limit as well as the monitoring of the dynamics in biological systems and processes under physiological conditions; however, certain limitations for AFM still exist in the field of bio-applications. In recent times, the development of another kind of scanning probe microscopy (SPM) technique, scanning ion conduc- tance microscopy (SICM), has overcome these limitations and enabled noninvasive, nanoscale investigation of live cells. SICM applications include imaging of cell topography, monitoring of live cell dynamics, mechanical stimulation of live cells, surface patterning, and so forth. We at Park Systems have developed AFM for advanced nanoscale metrol- ogy, which separates the z-scanner from the x-y scanner. An independent z-scanner also provides an excellent platform for developing other SPM techniques such as SICM. In addition, the platform which separates the z-scanner from x-y scanner, enables us to easily switch between an AFM and an SICM z-scanner to apply both techniques without moving samples. The common glass micropipette is used in SICM as the sensitive probe, instead of a silicon-based stylus, and can glide over live cells while maintaining an absolute non-contact imaging mode. Its electrochemical current feedback system further enhances biological sample imaging. Combining confocal fluorescence data to the SICM3D data, using an image overlay feature, pro- vides even more data about structure of cells as related to their membranes.

215

Biophysical Society 58 th Annual Meeting, San Francisco, California