Program Book

Here, we present the SURFE2R technology—an easy-to-handle, highly sensitive and very efficient screening platform for direct measurements of ion transporters and ion channels in diverse and heterologous membranes. Since 2012, Nanion offers the SURFE2R product line in two formats: SURFE2R N1 and the higher throughput platform SURFE2R N96. The SURFE2R N1 which we will present at the workshop is a small footprint, fully automated device recording from membrane prepara- tions, with proven success using native tissue, mammalian and insect cell lines, bacteria, organelles, and proteoliposomes. Come to our workshop and learn from LIVE-experiments how to make mea- surements of transporter-protein functionality efficient and reliable! Presenters: Andrea Brüggemann, CSO, Nanion Technologies Maria Barthmes, Nanion Technologies 1:00 pm–2:30 pm Molecular Devices, LLC Axon Electrophysiology Symposium: Getting the Most out of pCLAMP Software pCLAMP™ is a powerful data acquisition and analysis software and is widely used for a variety of electrophysiological recordings. In the first tutorial of this workshop, Jeffrey Tang will highlight a few features used to create a customized acquisition protocol in Clampex. In the second tuto- rial, Burt Maertz will share tips in single-channel analysis using Clampfit. These include burst analysis, latency analysis and P(open) analysis Presenters Jeffrey Tang, Product Marketing Manager, Axon Conventional Electrophysiology, Molecular Devices, LLC. Burt Maertz, Technical Support Specialist, Axon Conventional Electrophysiology, Molecular Devices, LLC.

Tuesday, February 18 9:00 am–10:30 am Wyatt Technology Corporation

Essential Biophysical Characterization ™ : Molar Mass, Size, Charge and Interactions—The Light Scattering Toolbox for Biomolecules and Nanoparticles Wyatt Technology provides the essential tools for characterization of biomacromolecules in solution, including peptides, proteins and oli- gonucleotides as well as bionanoparticles such as exosomes and VLP’s. This presentation describes the light scattering instrumentation and techniques used in these analyses: coupled to liquid chromatographic separations for absolute molar mass and size distributions (SEC/FFF- MALS); microtiter plate-based, high throughput screening of size, aggre- gation and interactions (DLS); and the label-free, immobilization-free analysis of biomolecular interactions for affinity and absolute molec- ular stoichiometry (CG-MALS). A variety of examples illustrate the unique capabilities of these light scattering measurements in biophysics. Presenters Chris Broomell, Applications Scientist Sophia Kenrick, Application Scientist, Wyatt Technology Corporation Precise Measurements of Membrane Transporter Protein Activity Ion transporters and pumps play an important role within general metabolism and information processing of organisms. Dysfunction and -regulation of transporter proteins are related to diseases like obe- sity, diabetes, hypertension, and CNS disorders such as epilepsy and depression. Hence, ion transporters have become potential targets within the drug development treating disease-related abnormalities. At present, labeling technologies and conventional patch clamp are commonly used for ion transporter screening. However, radioactive and fluorescence-based assays have limited sensitivity, and because of the limited molecule turnover per seconds of transporters and pumps compared to ion channels, the direct electrophysiological measurement of protein transporters and pumps activity is extremely challenging. 11:00 am–12:30 pm Nanion Technologies SURFE2R—Catch the Wave for Transporters

3:00 pm–4: 30 pm GE Healthcare

The Devil is in the Detail: the Importance of Accurate Stability and Concentration Determination in Biomolecular Interaction Analysis See Addendum for session description.

2014 Thematic Meetings

Modeling of Biomolecular Systems Interactions, Dynamics, and Allostery Istanbul, Turkey September 10–14, 2014 Abstract Deadline: May 5

Significance of Knotted Structures for Function of Proteins and Nucleic Acids Warsaw, Poland September 17–21, 2014 Abstract Deadline: May 12

Disordered Motifs and Domains in Cell Control Dublin, Ireland October 11–15, 2014 Abstract Deadline: June 2

217

Biophysical Society 58 th Annual Meeting, San Francisco, California