Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

17-POS Board 9 A Single-Molecule Approach to Study the Dendritic Function of the Rna-Binding Protein Tdp-43 Jen-Fei Chu , Pritha Majumder, Biswanath Chatterjee, Che-Kun James Shen. Academia Sinica, Taipei, Taiwan. The RNA-binding protein TDP-43 regulates RNA metabolism at multiple levels, including transcription, RNA splicing, and microRNA metabolism, as well as a major component of the cytoplasmic inclusions characteristic of amyotrophic lateral sclerosis and some types of frontotemporal lobar degeneration. We previously found that TDP-43 represses the translation initiation of Rac1 mRNAs through binding of TDP-43 to mRNA(s) at specific UG/GU sequences and consequently the hippocampal spinogenesis. However, direct evidence that TDP- 43 regulates Rac1 mRNAs in spatiotemporal manner has not been documented. We here conducted a multi-color localization based super-resolution microscopic and single particle tracking methods at the endogenous level to dissect the interaction of TDP-43 proteins and Rac1 mRNAs in live hippocampal neurons. It would allow us to extract messenger ribonucleoproteins (mRNPs) information that remains unclear about the TDP-43 protein-Rac1 mRNA complex in situ. This sheds new light on the pathology of mechanistic pathways induced by mis-metabolism of TDP-43 protein.

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