BPS Program Book 2014

10:45 am –12:45 pm , R oom 305 Platform Exocytosis and Endocytosis

179-P lat 11:30 am DYNAMIC EFFECTS OF TROPOMYOSIN D230N MUTATION AND FETAL TROPONIN T ON THE TROPOMYOSIN OVERLAP REGION. Mark T. McConnell , Jayant J. Jayasundar, Lauren Grinspan, Ofer Z. Fass, Benjamin Schwartz, Jil C. Tardiff 180-P lat 11:45 am IN SITU STRUCTURAL CHANGES IN THICK AND THIN FILAMENTS OF CARDIAC MUSCLE INDUCED BY FRAGMENTS OF MYOSIN BINDING PROTEIN C (MYBP-C). Thomas Kampourakis , Yin-Biao Sun, Mathias Gautel, Malcolm Irving 181-P lat 12:00 pm IMPACT OF FAMILIAL HYPERTROPHIC CARDIOMYOPATHY- LINKED MUTATIONS IN THE N-TERMINUS OF THE MYO- SIN REGULATORY LIGHT CHAIN ON THE CALCIUM BASED MOTILITY. Gerrie P. Farman , Priya Mutha, Katarzyna Kazmierczak, Danuta Szczesna-Cordary, Jeffery R. Moore 182-P lat 12:15 pm PHOSPHORYLATION MODULATES THE DYNAMICS OF THE N-TERMINAL TAIL IN CARDIAC RLC. Arianna Fornili , Elena Rostkova, Franca Fraternali, Mark Pfuhl 183-P lat 12:30 pm FAMILIAL HYPERTROPHIC CARDIOMYOPATHY-LINKED MUTATION (K104E) IN THE MYOSIN REGULATORY LIGHT CHAIN AFFECTS SARCOMERIC STRUCTURE AND FUNCTION IN TG-MICE. Wenrui Huang , Jingsheng Liang, Katarzyna Kazmierczak, Priya Muthu, Chen-Ching Yuan, Ana I. Rojas, Divya Duggal, Julian Borejdo, Thomas C. Irving, Danuta Szczesna-Cordary 11:00 am –12:00 pm , R oom 122 International Relations Committee Meeting Use- dependent inhibition of ion channels by potential drug candidates is an important aspect to investigate for many drug classes. Use-dependent drugs specifically target ion channels in cells that are more electrically active. For example, a drug targeting pain that is more potent to Na + chan- nels in neurons actively firing action potentials is a better drug candidate. Data will be presented to demonstrate the ability of automated electro- physiology systems to study the use-dependence block of Na + channel targets. Tetracaine, lidocaine, and TTX exhibit very different behavior in terms of their use- dependent blockage. We will demonstrate the ability of the instrumentation to deliver complex voltage protocols and gener- ate long assay windows which are required for these studies. Pulse trains delivered at 10Hz are used to measure the blockade of current. Data from a separate study will also be presented that demonstrate blockage and enhancement of NaV1.5 currents by various peptide toxins. Both sets of experiments demonstrate stable assay windows with uniform currents for 30 minutes and longer during the delivery of periodic pulse trains. Presenter: James Costantin, Product Marketing Manager, Automated Electrophysiology, Molecular Devices, LLC 11:00 am –12:30 pm , R oom 123 Exhibitor Presentation Molecular Devices, LLC Investigating Use-Dependent Inhibition of Ion Channels on Auto- mated Electrophysiology Systems including the IonWorks Barracuda ® Instrument and the IonFlux TM Benchtop Reader

F R I D A Y

S A T U R D A Y U N D A Y

Co-Chairs Jenny Hinshaw, NIDDK, NIH Diego Krapf, Colorado State University 168-P lat 10:45 am

withdrawn

169-P lat 11:00 am MOLECULAR DYNAMICS SIMULATIONS OF SNARE COMPLEX UNZIPPING. Satyan Sharma , Manfred Lindau 170-P lat 11:15 am CHOLESTEROL PROMOTES OPENING OF THE SNARE- MEDIATED FUSION PORE. Benjamin S. Stratton , Zhenyong Wu, Jason M. Warner, George Wei, Emma C. Wagnon, Erdem Karatekin, Ben O’Shaughnessy 171-P lat 11:30 am PROTEIN MOBILITY IN SECRETORY GRANULES AND FUSION PORE EXPANSION: FACTORS AFFECTING PROTEIN SECRETION. Annita Ngatchou-Weiss , Mary A. Bittner, Arun Anantharam, Daniel Axelrod, Ronald W. Holz 172-P lat 11:45 am NANOSTRUCTURE-INDUCED MEMBRANE CURVATURE RECRUITS ENDOCYTOSIS MACHINARY IN LIVING CELLS. Wenting Zhao , Lindsey Hanson, Ziliang Lin, Yi Cui, Bianxiao Cui 173-P lat 12:00 pm A DYNAMIN MUTANT DEFINES A SUPER-CONSTRICTED PRE-FISSION STEP. Anna Sunborger , Jurgen A. Heyman, Shunming Fang, Joshua S. Chappie, Jenny E. Hinshaw 174-P lat 12:15 pm CLATHRIN AGGREGATION BY ROTATIONAL BROWNIAN DYNAMICS. Ioana M. Ilie , Wouter K. den Otter, Wim J. Briels 175-P lat 12:30 pm QUANTIFYING THE DYNAMIC INTERACTIONS BETWEEN A CLATHRIN-COATED PIT AND CARGO MOLECULES. Aubrey V. Weigel, Michael M. Tamkun, Diego Krapf

10:45 am –12:45 pm , R oom 306 Platform Cardiac Muscle I

Co-Chairs Steven Schwartz, University of Arizona Gerrie Farman, Boston University 176-P lat 10:45 am

A REVISED ATOMISTIC MODEL OF THE CARDIAC THIN FILAMENT AND APPLICATION TO A SPECIFIC DISEASE CAUSING MUTATION. Michael R. Williams , Jil Tardiff, Steven Schwartz 177-P lat 11:00 am MOLECULAR MECHANISM FOR THE REGULATION OF CARDIAC MUSCLE CONTRACTION BY TROPONIN. Ivanka Sevrieva, Andrea Knowles, Yin-Biao Sun 178-P lat 11:15 am CALCIUM-SENSITIVE DYNAMIC EFFECTS OF TROPONIN’S TNI INHIBITORY REGION. Julie Mouannes Kozaili, Devanand Kowlessur, Larry S. Tobacman

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Biophysical Society 58 th Annual Meeting, San Francisco, California