BPS2025 Full Program

Platform Electron Microscopy 8:15 am - 10:15 am, Room 515B

Platform Protein-Nucleic Acid Interactions II 8:15 am - 10:15 am, Room 502A

W E D N

Co-Chairs T. Bertie Ansell, Stanford University, USA Jeremy Conway, Harvard University, USA 2417-Plat 8:15 am CRYOGENIC ELECTRON TOMOGRAPHY AND FLUORESCENCE LIGHT MI CROSCOPY OF MULTISPECIES COMMUNITIES WITHIN THE SOIL MICRO BIOME. T. Bertie Ansell , Louis Berrios, Kabir G. Peay, Peter D. Dahlberg 2418-Plat 8:30 am CRYOPROTECTANT-FREE LAMELLA PREPARATION OF PLANT AND ANI MAL TISSUE FOR CRYO-ELECTRON TOMOGRAPHY. Magda Zaoralova 2419-Plat 8:45 am TOMOSCORE: A NEURAL NETWORK APPROACH FOR QUALITY AS SESSMENT OF CELLULAR CRYO-ET. Xuqian Tan , Ethan Boniuk, Anisha Abraham, Steven Ludtke, Zhao Wang 2420-Plat 9:00 am CRYO-ELECTRON TOMOGRAPHY INVESTIGATION OF NATIVE AMPA RECEPTOR COMPLEXES WITHIN BRAIN TISSUE. Cathy J. Spangler , Aya Matsui, Johannes Elferich, Eric Gouaux 2421-Plat 9:15 am IN VITRO AND IN SITU CRYO-ET STUDY OF WT AND PD MUTANT LRRK2 ON MICROTUBULES. Siyu Chen , Tamar Basiashvili, Joshua Hutchings, Marta M. Sanz Murillo, Amalia C. Villagran Suarez, Jaime Alegrio Louro, Andres Leschziner, Elizabeth Villa 2422-Plat 9:30 am CLATHRIN LATTICE STRUCTURES IN PLASMA MEMBRANES GENER ATED BY A NOVEL CRYO-ELECTRON TOMOGRAPHY PIPELINE. Dennis J. Michalak , Willy W. Sun, Kem A. Sochacki, Jenny E. Hinshaw, Justin W. Taraska 2423-Plat 9:45 am VISUALIZING MITOCHONDRIAL BUDDING IN INTACT CELLS BY CRYO-EM AND CRYRO-ET. Guobin Hu , Judy Z. Hu, Lijun Qiao, Xianhai Zhao, Chang Jun Liu, David Chan 2424-FlashTalk 10:00 am SMALL-MOLECULE CATHODOPHORES FOR MULTICOLOR ELECTRON MICROSCOPY. Jeremy B. Conway , Sohaib Abdul Rehman, Jimmy Ching Cheng Hsu, Maxim B. Prigozhin 2425-FlashTalk 10:05 am COVET: CRYO-ELECTRON TOMOGRAPHY GUIDED BY OPTICAL ELECTRO PHYSIOLOGY. Soung-Hun Roh , Mingyu Jung 2426-FlashTalk 10:10 am THE INAUGURAL FLATIRON HETEROGENEITY COMMUNITY CHALLENGE. Miro A. Astore , Geoffrey Woollard, David Silva-Sánchez, Wenda Zhou, Misha Kopylov, Nikolaus Grigorieff, Khahn Dao Duc, Pilar Cossio, Sonya M. Hanson

Co-Chairs Vysakh Ramachandran, Iowa State University, USA Robert Silvers, Florida State University, USA 2427-Plat 8:15 am INTEGRATING ACCELERATED MOLECULAR SIMULATIONS AND AI TO ELU CIDATE CAS9 SPECIFICITY FACTORS. Gayatri Panda 2428-Plat 8:30 am HUMAN LA-RELATED PROTEIN 6 AND RNA RECOGNITION. Robert Silvers , Blaine Gordon 2429-Plat 8:45 am UNRAVELING RNA-DRIVEN BIOMOLECULAR CONDENSATE FORMATION. Vysakh Ramachandran 2430-Plat 9:00 am MECHANISM OF SARS-COV-2 NUCLEOCAPSID PROTEIN PHOSPHORY LATION-INDUCED FUNCTIONAL SWITCH. Ioulia F. Rouzina , Megan Sul livan, Michael Morse, Micah J. McCauley, Dina Bayachou, Jana Sefcikova, Mark C. Williams, Penny Beuning, Karin Musier-Forsyth 2431-Plat 9:15 am DECODING THE MOLECULAR MECHANICS OF MAMMALIAN TRANSCRIP TION COMPLEX FORMATION. Sreejith J. Nair 2432-Plat 9:30 am CHARACTERIZATION OF GUEST DNA TRANSPORT AND ADSORPTION WITHIN HOST POROUS PROTEIN CRYSTALS. Szu-Hsuan (Ashlyn) Chen , Julius Stuart, Brian Munsky, Christopher D. Snow 2433-Plat 9:45 am METABOLIC ENZYMES MOONLIGHTING AS RNA BINDING PROTEINS. Geordie Emberling, Nicole Curtis, Trang Tu, Kyle Zhang, Alquama Lokhandwala, Zoya Tajuddin, Ganesan Murugan, Mary Jo Ondrechen, Constance Jeffery 2434-FlashTalk 10:00 am UPF1 HELICASE EXHIBITS SEQUENCE-DEPENDENT KINETICS AS RE VEALED BY NANOPORE TWEEZERS. Francis J. Walsh 2435-FlashTalk 10:05 am TYPE II TOPOISOMERASE SUBSTRATE GEOMETRY REVEALED THROUGH COMBINED EXPERIMENT AND COMPUTATION. Mihirkumar N. Prajapati , Yeonee Seol, Jonathan E. Silver, Parth Rakesh Desai, Siddhar tha Das, Keir C. Neuman 2436-FlashTalk 10:10 am ANCESTRAL RNA-GUIDED TNPB NUCLEASE INTERROGATES DNA IN DIS CRETE SUPERCOILING-SENSITIVE STEPS. Iren Saffarian-Deemyad , Zehan Zhou, Kevin Aris, Honglue Shi, Noor Al-Sayyad, Jennifer A. Doudna, Zev Bryant

E S

D A Y

144