BPS2025 Full Program

912-Plat 12:15 pm THE STRUCTURE OF HUMAN K2P13.1 (THIK-1) CHANNEL REVEALS A NOVEL HYDROPHILIC PORE RESTRICTION AND LIPID COFACTOR SITE. Shatabdi Roy Chowdhury , Seil Jang, Fayal Abderemane-Ali, Fiona B. Naughton, Michael Grabe, Daniel L. Minor 913-FlashTalk 12:30 pm MOLECULAR AND STRUCTURAL BASIS OF THE REGULATION OF TRPP2 CHANNEL BY SMALL MOLECULE LIGANDS. Zhifei Wang, Mengying Chen, Qiang Su, Tiago Da Costa Morais, Yan Wang, Feng Qian, Yigong Shi, Yong Yu 914-FlashTalk 12:35 pm EVIDENCE OF DIFFERENTIAL EMERGENCE OF THE HUMAN TRPM8 CHANNEL COLD- AND MENTHOL-SENSING FUNCTIONS. Dustin D. Luu , Nikhil Ramesh, I. Can Kazan, Karan H. Shah, Gourab Lahiri, Miyeko D. Mana, S. Banu Ozkan, Wade D. Van Horn 915-FlashTalk 12:40 pm TRPM8 CHANNELS IN VASCULAR FUNCTION: INSIGHTS FROM KNOCK OUT MOUSE MODELS AND HUMAN GENETIC VARIANTS. M. Teresa Perez-Garcia , Diego A. Peraza, Lucia Alonso-Carbajo, Alice Giontella, Karel Talavera, Pilar Cidad, Olle Melander, Jose R. Lopez-Lopez Platform Protein-Lipid Interactions: Structure and Dynamics II 10:45 am - 12:45 pm, Room 511ABC Co-Chairs Richard Baker, University of North Carolina at Chapel Hill, USA Katelyn Kraichely, University of Virginia, USA 916-Plat 10:45 am A STRUCTURE-BASED MECHANISM FOR INITIATION OF AP-3 COATED VESICLE FORMATION. Matthew Begley, Richard Baker 917-Plat 11:00 am S-ACYLATION OF GASDERMINS REGULATES THEIR FUNCTION IN MEM BRANE PORE FORMATION AND PYROPTOSIS. Zhonghua Liu, Sai Li, Ch uanping Wang, Kaylynn J. Vidmar, Syrena Bracey, Ling Li, Belinda Willard, Masaru Miyagi, Tong Lan, Bryan C. Dickinson, Abdullah Osme, Theresa T. Pizarro, Tsan Sam Xiao 918-Plat 11:15 am SMALL-MOLECULE MODULATORS OF PROTEIN RAFT AFFINITY AND RAFT STABILITY. Katherine M. Stefanski , Dustin Luu, Joshua Bauer, Anne K. Kenworthy, Wade D. Van Horn, Charles R. Sanders 919-Plat 11:30 am DEMONSTRATING RAS-RAF PROTEIN CONFORMATIONAL TRANSITIONS FROM AUTOINHIBITION TO RAF-DIMERIZATION USING MACHINE LEARNING-DRIVEN MULTISCALE SIMULATIONS. Helgi I. Ingólfsson 920-Plat 11:45 am CHOLESTEROL BLOCKS THE ASSEMBLY AND ONCOGENIC SIGNALING OF THE EPHA2 RECEPTOR. Ryan J. Schuck, Alyssa E. Ward, Jennifer A. Rybak, Ioannis Sgouralis, Matthias Buck, Rajan Lamichhane, Francisco N. Barrera 921-Plat 12:00 pm A HIGH-THROUGHPUT PLATFORM FOR MAPPING THE NANOSCALE CARTOGRAPHY OF THE NATIVE MEMBRANE. Caroline Brown , Sneha sish Ghosh, Kallol Gupta 922-Plat 12:15 pm MECHANISM OF LIPID UPTAKE AND RELEASE BY STARD LIPID TRANSFER PROTEINS. Nathalie Reuter , Mahmoud Moqadam, Reza Talandashti, Parveen Gartan

923-FlashTalk 12:30 pm THE TWO SNARE MOTIFS OF THE NEURONAL SNARE SNAP25 HAVE DIS TINCT YET INTERDEPENDENT REGULATORY ROLES IN SNARE ASSEMBLY AND MEMBRANE FUSION. Katelyn N. Kraichely , Connor R. Sandall, Volker Kiessling, Binyong Liang, Lukas K. Tamm 924-FlashTalk 12:35 pm LIPID PACKING DEFECTS ARE NECESSARY AND SUFFICIENT FOR ALPHA SYNUCLEIN MEMBRANE BINDING. Miah J. Turke , Krishna M. Raghavan, Sofiya Maltseva, Daniel H. Kerr, Erin J. Adams, Ka Yee C. Lee 925-FlashTalk 12:40 pm Travel Award Winner GPCR SIGNAL TRANSDUCTION AFFECTED BY WATER AND LIPIDS. Sean J.X. Chen , Kushani S.K. Hewage, Helen F. Mann, Andrey V. Struts, Mi chael F. Brown Annual Meeting of the Student Chapters 11:00 am - 12:30 pm, Room 403B Join BPS Student Chapters from all over the world for a networking ses sion. Attendees will meet Student Chapter officers and representatives and learn about each chapter. This session aims to establish chapter interactions, communications, and planning for future Student Chapter Annual Meeting sessions. Exhibitor Presentation Nanion Technologies 11:30 am - 1:00 pm, Room 404AB Novel Insights into Ion Channel and Transporter Research: From Silent Translocation to Deep Learning Tools Speaker: Tim Strassmaier, Director of Scientific Operations, Nanion Technologies For over 2 decades, Nanion Technologies has been providing diverse solutions for electrophysiologists worldwide. We aim to implement innovative technologies in the fields of ion channel automated patch clamp (APC) electrophysiology, monitoring of cell viability and con tractility, as well as electrogenic transporters, with various throughput capabilities. Our symposium will start with an introduction by Dr. Tim Strassmaier who will describe the latest advances in Nanion’s assays and product portfolio, followed by our speakers, whose work focuses on ion channel and transporter research. Leveraging Deep Learning Tools to Design hASIC1a Modulators Speaker: Janina Sörmann, Postdoctoral Researcher, University of Copen hagen Stroke is a leading cause of death and adult disability. Acid-sensing ion channels (ASICs) in the central nervous system contribute to tissue damage after ischemic stroke but are underexplored therapeutic targets due to the lack of potent, selective, and stable inhibitors. During this presentation, Dr. Sörmann will describe de novo designed miniproteins capable of inhibiting hASIC1a by integrating deep learning tools in the drug discovery process and combined with functional analysis on the SyncroPatch 384. These computationally designed modulators offer high specificity, efficacy, and cost-effective synthesis, broadening accessibility for stakeholders.. Benefits of SSME for the Investigation of the Lysosomal Ion Channels TMEM175, TPC2, and TRPML1 Speaker: Rocco Zerlotti, Application Scientist, Nanion Technologies Solid-supported membrane-based electrophysiology (SSME) is a tech nique for studying transport and electrogenic activities in transporters, pumps, and ion channels, including those located in inner membranes such as mitochondria and lysosomes. Using a fluidic system for rapid solution exchange, transport or ion conductance are driven by substrate concentration gradients at zero membrane potential. Dr. Zerlotti will present a recent study focused on investigation of lysosomal channels

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